Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,035,029 T→C 6.1% intergenic (+845/+596) pgi‑I → / ← PP_5477 glucose 6‑phosphate isomerase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,035,0290TC 6.1% 193.3 / 2.4 65intergenic (+845/+596)pgi‑I/PP_5477glucose 6‑phosphate isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/36);  new base C (2/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

AGATCGCAAAGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTCCCT  >  NC_002947/2034885‑2035175
                                                                                                                                                 |                                                                                                                                                  
aGATCGCAAAGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAAc                                                                                                                                                >  3:335683/1‑149 (MQ=255)
   tcggttaGCTGGGGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGGCAGTAAAGAATGACCTTTTGCGTAAAGCGTGATATTCTCAA‑TCCTATGTTACGGGATCTCCCTATAGACAGCCAGTGAACCt                                                                                                                                              <  2:291537/142‑1 (MQ=255)
   tCGCAAAGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCt                                                                                                                                              <  5:264514/148‑1 (MQ=255)
      cAAAGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCAcc                                                                                                                                          <  6:2257/149‑1 (MQ=255)
       aaaGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCAccc                                                                                                                                         >  6:309940/1‑149 (MQ=255)
          gCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCtt                                                                                                                                       >  3:280462/1‑148 (MQ=255)
              gcgcTGACACGCGGTGCGATCTTGGTTGAAATTTCGTAACTGAGTGTCAAATTGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAACTCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCt                                                                                                                                    <  1:276371/148‑1 (MQ=255)
              gcgcTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGTCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTAt                                                                                                                                  <  2:26902/149‑1 (MQ=255)
              gcgcTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTa                                                                                                                                   <  8:152353/148‑1 (MQ=255)
              gcgcTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTa                                                                                                                                   >  3:171637/1‑148 (MQ=255)
                gcTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATc                                                                                                                                 >  8:289823/1‑148 (MQ=255)
                gcTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCa                                                                                                                                <  4:47412/149‑1 (MQ=255)
                    acacGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAg                                                                                                                              <  5:309940/147‑1 (MQ=255)
                    acacGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGATACGGCATCACCCTATACACAGCCAGTGAACCCCACCCTTTCTATCAAGaa                                                                                                                            <  4:319644/149‑1 (MQ=255)
                      acGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAg                                                                                                                          >  8:122621/1‑149 (MQ=255)
                      acGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAg                                                                                                                          >  2:67248/1‑149 (MQ=255)
                         cgGTTCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCa                                                                                                                       <  4:181432/149‑1 (MQ=255)
                              cGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAgg                                                                                                                   <  4:280462/148‑1 (MQ=255)
                                     ggTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTc                                                                                                                 <  7:289823/143‑1 (MQ=255)
                                     ggTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTc                                                                                                                 <  4:293723/143‑1 (MQ=255)
                                     ggTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGAc                                                                                                              <  4:335683/146‑1 (MQ=255)
                                     ggTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGAc                                                                                                              <  2:302500/146‑1 (MQ=255)
                                      gTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGAt                                                                                                          <  2:54442/149‑1 (MQ=255)
                                       tAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGAt                                                                                                          <  7:74894/148‑1 (MQ=255)
                                       tAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATg                                                                                                         >  3:319077/1‑149 (MQ=255)
                                             tttCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTc                                                                                                                        <  5:111814/128‑1 (MQ=255)
                                             tttCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCACTGAACCTCACCCTTTCTATCAAGAAAGta                                                                                                                        >  6:111814/1‑127 (MQ=255)
                                                      tGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCcg                                                                                          >  3:352470/1‑149 (MQ=255)
                                                              aaaTGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACacag                                                                                  >  5:326479/1‑149 (MQ=255)
                                                              aaaTGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACacag                                                                                  <  4:319077/149‑1 (MQ=255)
                                                                            cAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGt                                                                    <  4:205504/149‑1 (MQ=255)
                                                                             aaaGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTAt                                                                                                      >  1:79379/1‑114 (MQ=255)
                                                                             aaaGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTAt                                                                                                      <  2:79379/114‑1 (MQ=255)
                                                                             aaaGACCTTTTGCGTAAAGCGTGATATTAACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCTACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGtt                                                                   <  7:203473/149‑1 (MQ=255)
                                                                                gACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTAcc                                                                <  2:314114/149‑1 (MQ=255)
                                                                                    ttttGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTAcc                                                                <  8:184849/145‑1 (MQ=255)
                                                                                    ttttGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTAcc                                                                >  7:184849/1‑145 (MQ=255)
                                                                                    ttttGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTc                                                             >  8:253090/1‑148 (MQ=255)
                                                                                    ttttGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACACTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTc                                                             >  3:21130/1‑148 (MQ=255)
                                                                                      ttGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTa                                                           <  1:67248/148‑1 (MQ=255)
                                                                                          gTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGtt                                                      >  7:205887/1‑149 (MQ=255)
                                                                                           tAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTg                                                     <  4:171637/149‑1 (MQ=255)
                                                                                                  tGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTAc                                                          <  3:37287/137‑1 (MQ=255)
                                                                                                  tGATATTCAAAA‑TCATATGTTACGGCATCACCATATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACAGATTTATTAGTCTATTCCTCGACACAAGACAGGCACACACATACCTTCTAc                                                          >  4:37287/1‑137 (MQ=255)
                                                                                                     tatTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCtggttgg                                           <  4:21130/149‑1 (MQ=255)
                                                                                                     tatTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCtggttg                                            <  8:205887/148‑1 (MQ=255)
                                                                                                          acaA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTa                                                           <  3:148310/128‑1 (MQ=255)
                                                                                                          acaA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTa                                                           >  4:148310/1‑128 (MQ=255)
                                                                                                          acaA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATg                                      >  2:188796/1‑149 (MQ=255)
                                                                                                               tCCTATGTTTCGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGAGTCCGAGCCACAGGACAGGCCCCCAGTTACCTTCGACCGTTGGGCTGGTTGGTgctgctgt                                  >  2:202635/1‑147 (MQ=255)
                                                                                                                 cTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCAGGTTGGTGATGCTCTCg                                >  1:212665/1‑149 (MQ=255)
                                                                                                                  tATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGCGCTGGTTGGTGATGCTCTCgg                               >  1:345254/1‑149 (MQ=255)
                                                                                                                  tATGTTACGGCATCACCCTATAGACAGCCAGCGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCgg                               >  3:296855/1‑149 (MQ=255)
                                                                                                                  tATGTTACGGCATCACCCTATAGACAGCCAGCGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCgg                               >  3:291806/1‑149 (MQ=255)
                                                                                                                    tGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCCCCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCggg                              >  6:174716/1‑148 (MQ=255)
                                                                                                                    tGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGCGCTGGTTGGTGATGCTCTCgg                               <  2:345254/147‑1 (MQ=255)
                                                                                                                            cATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTg                     <  7:122621/149‑1 (MQ=255)
                                                                                                                            cATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTg                     <  7:253090/149‑1 (MQ=255)
                                                                                                                                   cTATAGACAGCCAGCGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCggg              <  4:296855/149‑1 (MQ=255)
                                                                                                                                   cTATAGACAGCCAGCGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCggg              <  4:291806/149‑1 (MQ=255)
                                                                                                                                            gCCAGTGTACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTGCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCACTCGGGGATGTTCTg                     <  3:18933/133‑1 (MQ=255)
                                                                                                                                              cAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTccc   <  1:188796/149‑1 (MQ=255)
                                                                                                                                              cAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTccc   <  4:352470/149‑1 (MQ=255)
                                                                                                                                               aGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTCCCt  >  2:508/1‑149 (MQ=255)
                                                                                                                                               aGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTCCCt  >  1:159362/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGATCGCAAAGCTGGCGCTGACACGCGGTGCGATCTTGGTAGAAATTTCGTAACTGAGTGTCAAATGGCCAGTAAACAAAGACCTTTTGCGTAAAGCGTGATATTCACAA‑TCCTATGTTACGGCATCACCCTATAGACAGCCAGTGAACCTCACCCTTTCTATCAAGAAAGTCAGAGGTCGACCGATGTATTAGTCGATTCCGCGACACAGGACAGGCCCACAGTTACCTTCTACCGTTGGGCTGGTTGGTGATGCTCTCGGGGATGTTCTGGGCCGGGGGAGCGCTCCCT  >  NC_002947/2034885‑2035175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑