| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,049,304 | A→C | 11.4% | intergenic (‑33/+27) | PP_1822 ← / ← folEA‑I | hypothetical protein/GTP cyclohydrolase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,049,304 | 0 | A | C | 11.4% | 137.5 / 7.9 | 51 | intergenic (‑33/+27) | PP_1822/folEA‑I | hypothetical protein/GTP cyclohydrolase I |
| Reads supporting (aligned to +/- strand): ref base A (22/23); new base C (3/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CTTGCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGCA > NC_002947/2049157‑2049447 | cTTGCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGaa < 5:189069/149‑1 (MQ=255)cTTGCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGaa < 6:238690/149‑1 (MQ=255) gCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAg < 7:212721/147‑1 (MQ=255) cTCGAGGCGGGCCTGGGCGGCGGCGTCGCGGTTGCGCGTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGt < 8:106876/149‑1 (MQ=255) gcGATGCGGGCCTGGGCGGCGGCGGCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGACGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTAACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTc < 8:262501/148‑1 (MQ=255) gATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGCGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTc < 1:66585/148‑1 (MQ=255) gATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTc < 3:108599/147‑1 (MQ=255) cGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCg > 7:28703/1‑149 (MQ=255) gggCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCgg > 4:103664/1‑149 (MQ=255) gCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCtttt > 8:182038/1‑144 (MQ=255) gCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCtttt < 7:182038/147‑4 (MQ=255) tctcgTTGGCGCTGGGCCGGGCGGCTGCTCCAGGCACCGCAGACGCTGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCCTGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGACGGGTCGGCTCGGGGCCAGCAACCAATCAc < 8:178039/146‑1 (MQ=255) cgTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTg < 3:103664/149‑1 (MQ=255) ccTTGGCCTGGAGGCAGATCCAGTCACCGAACACGATGAGCTGGCCGAGGCCAACCCGCAGGGCATTGACGATCATCT‑CCAACTCCTGAAATGCAAAAGCCGACCATGAAGGGTCGGCTCGGGGCCAGCAACAAATCACATGATCAGGc > 4:145992/2‑149 (MQ=255) ttGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCt > 6:310010/1‑148 (MQ=255) ccGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAgg > 7:252809/1‑148 (MQ=255) cggcTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCg < 6:138796/149‑1 (MQ=255) cAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCgg < 6:149553/146‑1 (MQ=255) cAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCgg > 5:149553/1‑146 (MQ=255) aCCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTggcggc > 3:6745/1‑149 (MQ=255) cGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCtttt > 6:118978/1‑92 (MQ=255) cGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCtttt < 5:118978/95‑4 (MQ=255) cGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGt > 3:95911/1‑149 (MQ=255) gACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTc < 3:145992/149‑1 (MQ=255) cGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTcgc < 8:252809/149‑1 (MQ=255) gATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTcgcg < 8:28703/149‑1 (MQ=255) gCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACt > 4:54064/1‑149 (MQ=255) ggCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCAc > 5:85141/1‑149 (MQ=255) cGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCAcc > 5:102037/1‑147 (MQ=255) aGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGc < 3:54064/149‑1 (MQ=255) cAACCCGCACGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCATGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGCACTCACCCAGCATCa > 8:103882/1‑149 (MQ=255) aCCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCAcc < 4:6745/149‑1 (MQ=255) ccGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGa < 6:85141/149‑1 (MQ=255) gggCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGa < 4:95911/149‑1 (MQ=255) tttGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACACAGCATCACCGAGGTGATCAt > 2:43067/1‑149 (MQ=255) tGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATg < 6:102037/148‑1 (MQ=255) cAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCt > 1:315353/1‑148 (MQ=255) aaCTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTtc > 3:354726/1‑149 (MQ=255) cTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTtctc < 5:310010/149‑1 (MQ=255) gAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGacac > 7:9082/1‑149 (MQ=255) aTGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACcgc > 6:263271/1‑149 (MQ=255) aTGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACcgc < 4:354726/149‑1 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCt < 7:267947/130‑1 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCt > 8:267947/1‑130 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcat > 7:270158/1‑149 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGAGGAACTCACCAAGCATCACCGAGGTGAGCATGGTCGAATTCTGCTTCTAGAAACCGCGcat > 6:208932/1‑149 (MQ=255) aaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatc < 5:263271/149‑1 (MQ=255) aaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatc < 8:9082/149‑1 (MQ=255) aaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcat > 5:21984/1‑148 (MQ=255) ccGACCCTTCAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACcgc < 5:182951/140‑1 (MQ=255) ccGACCCTTCAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACcgc > 6:182951/1‑140 (MQ=255) aCCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGc < 2:315353/149‑1 (MQ=255) ccTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGca > 5:355089/1‑148 (MQ=255) | CTTGCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGG‑GCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGCA > NC_002947/2049157‑2049447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |