Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,053,642 G→A 7.4% intergenic (‑42/+24) PP_1828 ← / ← PP_1829 hypothetical protein/alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,053,6420GA 7.4% 192.6 / 4.2 67intergenic (‑42/+24)PP_1828/PP_1829hypothetical protein/alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base A (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01

GCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCGCAGC  >  NC_002947/2053494‑2053789
                                                                                                                                                    |                                                                                                                                                     
gcCAGGCGTCGCAGCTGGAGATGTGCGGCGGTCCGCGCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGCCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATg                                                                                                                                                       <  8:213137/149‑1 (MQ=255)
 ccAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATgg                                                                                                                                                      >  7:272800/1‑149 (MQ=255)
  ctggCGTCGCAGCGGGAAAGGTGCGCCGGCCCCCGCGCCCCAGGGCCGGGGGGGGGTTTCCGGGGGGTCGGCGGCCGGCGCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGc                                                                                                                                                     <  3:350957/147‑1 (MQ=255)
   aGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCt                                                                                                                                                    >  5:375916/1‑149 (MQ=255)
     gCGTAGCTGCTGGAAATGTGCGGCGGTCAGCTCGGCCCAGGGCTGGGCGGGGGTTTCCTGGGGGTCTTCGGCCGGCGCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTga                                                                                                                                                  <  2:140905/149‑1 (MQ=255)
     gCGTAGCAGCTGGAAATGTGCGGCGGTCAGCGCGGCCCCGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCCGATACAGCAGGCATAGCTga                                                                                                                                                  <  3:111285/149‑1 (MQ=255)
            aggtGGAAATGTTGGGGGTGCAGCTGGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGCCGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                                           <  6:299966/146‑1 (MQ=255)
             gcTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAg                                                                                                                                          >  1:19669/1‑149 (MQ=255)
                   aaggtccgggggTCTCCTCGGCCCTGGGACGGGCGGGGGTTGGCGGGGGTTCTGCGGCCGGCTGGCCGGGGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑t                                                                                                                                   <  8:220065/142‑1 (MQ=255)
                       ttCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGa                                                                                                                               <  7:351193/148‑1 (MQ=255)
                       ttCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGa                                                                                                                               <  4:241133/148‑1 (MQ=255)
                          ggcggGCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTa                                                                                                                           >  7:331980/1‑149 (MQ=255)
                              tctaGCGCGGCCCCGGGCCGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACg                                                                                                                         <  4:10187/144‑1 (MQ=255)
                                  gCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACg                                                                                                                         >  3:10187/1‑143 (MQ=255)
                                  gCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAg                                                                                                                   <  8:331980/149‑1 (MQ=255)
                                        cccAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAAt                                                                                                             >  4:141702/1‑149 (MQ=255)
                                           agggcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCt                                                                                                          >  8:320053/1‑149 (MQ=255)
                                             ggcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTg                                                                                                         >  2:161850/1‑148 (MQ=255)
                                                agggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGAt                                                                                                      >  3:320875/1‑148 (MQ=255)
                                                   gcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAAc                                                                                                  >  8:286443/1‑149 (MQ=255)
                                                   gcGGGGGTTTCCTGGGGTGCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGTCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAAc                                                                                                  <  3:44423/149‑1 (MQ=255)
                                                       ctgTTTCCTTGGGTTCTTCGGCCGGCTCTACGGCGGTTGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACGTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTc                                                                                               <  4:120453/146‑1 (MQ=255)
                                                        ggTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTcac                                                                                             <  8:272800/149‑1 (MQ=255)
                                                         gTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTcac                                                                                             >  4:320071/1‑148 (MQ=255)
                                                          tttCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTc                                                                                               >  3:120453/1‑145 (MQ=255)
                                                                  ggTTCTTCGGCCGGCTCGACGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgctcg                                                                                   <  6:375916/149‑1 (MQ=255)
                                                                      cttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCa                                                                               >  2:283426/1‑149 (MQ=255)
                                                                         cggccggcTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAAt                                                                                          >  6:332265/1‑135 (MQ=255)
                                                                         cggccggcTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAAt                                                                                          <  5:332265/135‑1 (MQ=255)
                                                                           gccggcTCGACGGAGGTGGGGGAGGTCGTCAACAGGCAATCTACTCACCTATGCCTGCTGTATCGGCACGCATGGCTGAGAACTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGCATGAACCTCACAATACGCTCGCCCAGCtaa                                                                          >  7:14748/1‑147 (MQ=255)
                                                                            ccggcTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCAc                                                                         <  2:19669/149‑1 (MQ=255)
                                                                            ccggcTCGACGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTTAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCAc                                                                         <  8:62623/149‑1 (MQ=255)
                                                                                cTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACgcgc                                                                     <  3:141702/149‑1 (MQ=255)
                                                                                            gggggAGGTGGTCATCAGGGCCTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTc                                                         >  8:42282/1‑149 (MQ=255)
                                                                                               ggAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCt                                                          <  6:361079/145‑1 (MQ=255)
                                                                                               ggAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCt                                                          >  5:361079/1‑145 (MQ=255)
                                                                                               ggAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCggg                                                      <  1:52902/149‑1 (MQ=255)
                                                                                                  ggtggtCATCAGGGCGTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGtt                                                   <  8:312438/149‑1 (MQ=255)
                                                                                                   gtggtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAg                                                            >  7:296261/1‑139 (MQ=255)
                                                                                                   gtggtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAg                                                            <  8:296261/139‑1 (MQ=255)
                                                                                                    tggtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGa                                                 <  7:320053/149‑1 (MQ=255)
                                                                                                      gtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               >  5:248253/1‑149 (MQ=255)
                                                                                                       tcatcaGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               >  1:52685/1‑148 (MQ=255)
                                                                                                              ggCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTc                                       <  3:320071/149‑1 (MQ=255)
                                                                                                                    tCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctg                                 >  4:244646/1‑149 (MQ=255)
                                                                                                                     tcTCAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGCGTTGAGGTAGCTGTCGCgctgc                                >  4:40849/2‑149 (MQ=38)
                                                                                                                      cTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgct                               >  8:46762/1‑149 (MQ=255)
                                                                                                                       tCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCGTG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgct                               >  1:76238/1‑149 (MQ=255)
                                                                                                                       tCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGctgctg                              >  5:191903/1‑149 (MQ=255)
                                                                                                                       tCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGctgctg                              >  2:355189/1‑149 (MQ=255)
                                                                                                                       tCAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTcgc                                     >  3:91988/1‑142 (MQ=38)
                                                                                                                       tCAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTcgc                                     <  4:91988/142‑1 (MQ=38)
                                                                                                                         aGCCATGCCTGCCGATACAGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGc                            >  7:34000/1‑149 (MQ=255)
                                                                                                                           cTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTc                          <  7:286443/149‑1 (MQ=255)
                                                                                                                                  tgctgTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               <  7:94063/121‑1 (MQ=255)
                                                                                                                                  tgctgTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               >  8:94063/1‑121 (MQ=255)
                                                                                                                                  tgctgTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCt                   >  4:358220/1‑149 (MQ=255)
                                                                                                                                    ctgTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCtt                  <  7:46762/148‑1 (MQ=255)
                                                                                                                                     cgatacagcaggcaTAGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgg                <  8:131909/142‑1 (MQ=255)
                                                                                                                                       tATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgg                <  1:283426/147‑1 (MQ=255)
                                                                                                                                         tCGGCAGGCATGGCTGAGACCTGAGCC‑TGATTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGc             >  1:27164/1‑149 (MQ=255)
                                                                                                                                         tCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGc             <  1:161850/148‑1 (MQ=255)
                                                                                                                                          cagcaggcaTGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTg           <  8:34000/147‑1 (MQ=255)
                                                                                                                                           ggcaggcaTGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCAt                        <  6:242391/135‑1 (MQ=255)
                                                                                                                                           ggcaggcaTGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCAt                        >  5:242391/1‑135 (MQ=255)
                                                                                                                                            gcaggctTGGCTGTGTGCTTAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCATCTGACGCGCGAGCGGCATTTCGGGTTTGAGGTAGCTGTCGCGCTTCTGGCTCATGCCCTTGGGTCTGAt         <  8:14748/149‑1 (MQ=255)
                                                                                                                                            gcaggcaTGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGAt         >  7:143703/1‑149 (MQ=255)
                                                                                                                                                 caTGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCgca    <  3:244646/149‑1 (MQ=255)
                                                                                                                                                   tGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCgcagc  <  4:320875/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCC‑TG‑TTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCGCAGC  >  NC_002947/2053494‑2053789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑