Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,082,283 A→T 9.4% D317E (GAT→GAA)  PP_1861 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,082,2830AT 9.4% 155.2 / 2.8 55D317E (GAT→GAA) PP_1861LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/28);  new base T (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAGGCAA  >  NC_002947/2082137‑2082429
                                                                                                                                                  |                                                                                                                                                  
tcaGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATc                                                                                                                                                  <  6:157951/149‑1 (MQ=255)
      aCGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAATTTCTGGCACGCTGATCATCAGTGCGCGATTAATTCGCGCac                                                                                                                                            <  4:312553/149‑2 (MQ=255)
      aCGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGATTAATTCGCGCac                                                                                                                                            <  5:346802/149‑2 (MQ=255)
              gAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTgc                                                                                                                                    <  5:376340/149‑1 (MQ=255)
              gAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTgc                                                                                                                                    >  5:138216/1‑149 (MQ=255)
                   cAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCaa                                                                                                                                <  5:335705/148‑1 (MQ=255)
                      ggCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGc                                                                                                                             >  2:32395/1‑148 (MQ=255)
                          aaaCAGACCTTGTTGTCCAGCAGCAGCTCGTCACAGGAGTCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGt                                                                                                                        <  7:44086/149‑1 (MQ=255)
                           aaCAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTc                                                                                                                       >  8:323191/1‑149 (MQ=255)
                           aaCAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTc                                                                                                                       >  7:149218/1‑149 (MQ=255)
                                aCCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAgg                                                                                                                  <  7:312358/149‑1 (MQ=255)
                                aCCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAgg                                                                                                                  >  5:57818/1‑149 (MQ=255)
                                  cTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGTGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGt                                                                                                                <  2:332566/149‑1 (MQ=255)
                                  cTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTATTc                                                                                                                                                  <  5:195472/115‑1 (MQ=255)
                                  cTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAGTc                                                                                                                                                  >  6:195472/1‑115 (MQ=255)
                                  cTGGGTGTCCAGCAGCAGCTAGTCACAGGAGGCTTGGGTTTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCCCGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGt                                                                                                                <  4:64067/149‑1 (MQ=255)
                                   tGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTg                                                                                                               >  6:174521/1‑149 (MQ=255)
                                     gTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTg                                                                                                               <  8:149218/147‑1 (MQ=255)
                                      ttGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGaa                                                                                                            <  3:330651/149‑1 (MQ=255)
                                            agcagcagcTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGc                                                                                                      >  3:150674/1‑149 (MQ=255)
                                                    cTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCgg                                                                                              <  6:138216/149‑1 (MQ=255)
                                                       gTCACAGGAGGCTTGGGCGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGctc                                                                                           <  7:44636/149‑1 (MQ=255)
                                                        tCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCAGGCTGATCATAAGTGCGCGATTTAATCGCGCAATTCACTGCGCAAGGACAGGTCCCAGGGTGGAATCGGGCTGAAGCGGctct                                                                                          <  1:107494/149‑1 (MQ=255)
                                                           caGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGa                                                                                       <  8:11251/149‑1 (MQ=255)
                                                                   ttGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTc                                                                                >  6:318953/1‑148 (MQ=255)
                                                                   ttGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTc                                                                                >  8:278406/1‑148 (MQ=255)
                                                                     gggTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGTATCGGGCTGAAGCGGCTCTTGAGGAATTCg                                                                               <  1:32395/147‑1 (MQ=255)
                                                                      ggTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGagc                                                                            <  6:255060/149‑1 (MQ=255)
                                                                       gtgtCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGagca                                                                           <  5:318953/149‑1 (MQ=255)
                                                                            ggCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCaaa                                                                      >  4:254909/1‑149 (MQ=255)
                                                                                  cATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTg                                                                >  6:164365/1‑149 (MQ=255)
                                                                                   aTGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGa                                                                                       >  2:43000/1‑125 (MQ=255)
                                                                                   aTGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGa                                                                                       <  1:43000/125‑1 (MQ=255)
                                                                                   aTGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGc                                                               <  6:57818/149‑1 (MQ=255)
                                                                                            cTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTc                                                       <  5:164365/148‑1 (MQ=255)
                                                                                             ttGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGt                                                     >  3:270882/1‑149 (MQ=255)
                                                                                             ttGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGt                                                     >  5:118183/1‑149 (MQ=255)
                                                                                                      atcaATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGc                                            <  3:26458/149‑1 (MQ=255)
                                                                                                         aaTGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAg                                         <  4:150674/149‑1 (MQ=255)
                                                                                                          aTGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAgg                                        >  3:337826/1‑149 (MQ=255)
                                                                                                           tGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGc                                       >  5:283647/1‑149 (MQ=255)
                                                                                                              aaaaTTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTa                                    <  6:283647/149‑1 (MQ=255)
                                                                                                                  ttCTTGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAgcgc                                <  4:216005/149‑1 (MQ=255)
                                                                                                                  ttCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAgcgc                                >  7:198051/1‑149 (MQ=255)
                                                                                                                        cACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAAt                          <  4:337826/149‑1 (MQ=255)
                                                                                                                               atcatcAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCt                    <  7:323191/148‑1 (MQ=255)
                                                                                                                                  atcaGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAGACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGt                 <  5:174521/148‑1 (MQ=255)
                                                                                                                                   tcaGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTGCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGtct               >  5:1261/1‑149 (MQ=255)
                                                                                                                                     attGCGCGATTAATTCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGtct               >  7:151789/3‑147 (MQ=255)
                                                                                                                                     attGCGCGATTAATTCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGACAGGAGTAGCGCGTAAATGCCGCTGGtct               >  3:253334/3‑147 (MQ=255)
                                                                                                                                      gTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCgaga                                                           <  4:144786/102‑1 (MQ=255)
                                                                                                                                      gTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCgaga                                                           >  3:144786/1‑102 (MQ=255)
                                                                                                                                        gcgcGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCt          >  8:352584/1‑149 (MQ=255)
                                                                                                                                            gAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGtt                                                    <  6:353013/103‑1 (MQ=255)
                                                                                                                                            gAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGtt                                                    >  5:353013/1‑103 (MQ=255)
                                                                                                                                                 aaTCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAGGCaa  <  4:270882/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCAGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAGGCAA  >  NC_002947/2082137‑2082429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑