| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,211,906 | T→G | 7.7% | F13C (TTC→TGC) ‡ | PP_1955 → | cytochrome P450 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,211,906 | 0 | T | G | 7.7% | 155.0 / 4.6 | 52 | F13C (TTC→TGC) ‡ | PP_1955 | cytochrome P450 family protein |
| Reads supporting (aligned to +/- strand): ref base T (24/24); new base G (2/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
GGCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTG > NC_002947/2211758‑2212050 | gcgctctTCGGCAATCTAACTTAAATATAGATTTCCTCAGCGTAATGTAGAGATACTTTCTTGGTGGGATGCCGCGT‑TCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGTACGTCCTCAAG‑CGCCATCGGACtt < 2:98316/146‑1 (MQ=255) tctGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAAt > 4:47206/1‑149 (MQ=255) tctGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAAt > 5:78691/1‑149 (MQ=255) ggCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTCGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGAt < 3:112932/149‑1 (MQ=255) cACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGATTGAAGTCCGAtgg < 2:304196/149‑2 (MQ=255) ctctAAGTTAAATCTAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGAtgagt < 6:222296/149‑1 (MQ=255) tctAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACGTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCGGAtgagtg > 8:151248/1‑149 (MQ=255) tAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGTCGCCATCGGACTTCAATCCGAtgagt < 1:4170/147‑1 (MQ=255) aGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCa < 1:332983/149‑1 (MQ=255) taGATTTCCTCAGCGTAATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTc < 4:290955/148‑1 (MQ=255) cTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGGCCTCAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGGGCAGAGTTTTCGTGATc > 1:256406/1‑147 (MQ=255) cTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCa < 4:26231/148‑1 (MQ=255) cTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAg > 5:41988/1‑149 (MQ=255) tCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGc > 1:365241/1‑149 (MQ=255) cAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGcc > 3:286261/1‑149 (MQ=255) tAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGc < 3:47206/148‑1 (MQ=255) gTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAg > 7:199052/1‑149 (MQ=255) acttacttGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTgc > 5:211935/1‑149 (MQ=255) ttagttGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTAGATCGTCCTCAAG‑CGCCATCGGACTTCAATCAGATGAGTGAGAAGAGTTTTCGTGATCATGCATCAAACTGCCAGCGTga > 5:84358/1‑146 (MQ=255) ttGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGcgcgcg > 1:211159/1‑148 (MQ=255) ggtggGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCgaa < 4:86868/148‑1 (MQ=255) ggtggGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCgaa < 2:365241/148‑1 (MQ=255) gTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGAtgag > 8:117112/1‑94 (MQ=255) gTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGAtgag < 7:117112/94‑1 (MQ=255) gTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAAcc < 4:286261/149‑1 (MQ=255) gCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAAcccc < 6:78691/148‑1 (MQ=255) aGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTAt < 6:171645/149‑1 (MQ=255) ggAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTAt < 7:293674/148‑1 (MQ=255) gAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATgc > 7:190888/1‑149 (MQ=255) atTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGcgccgc > 5:276498/1‑148 (MQ=255) ttGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGcgccgc > 3:248904/1‑147 (MQ=255) ttCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGc < 2:211159/149‑1 (MQ=255) gagaATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCgtgt < 6:41988/149‑1 (MQ=255) aaTAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCCGCCTCAATCTGCCAGCGTGCGCGCGAAGAGACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGa > 2:370045/1‑149 (MQ=255) aCAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGGGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATgg < 7:151248/148‑1 (MQ=255) aTGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAAcgcg > 1:204578/1‑149 (MQ=255) aaaTACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGAGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTAt > 4:304077/1‑116 (MQ=255) aaaTACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGAGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTAt < 3:304077/116‑1 (MQ=255) tACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGagaa < 4:248904/149‑1 (MQ=255) aTCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGcc < 8:199052/149‑1 (MQ=255) tCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCg > 5:49133/1‑149 (MQ=255) cAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCCTTTTTCTATGCGCCGCTAGGCgtgt < 1:5181/116‑1 (MQ=255) cAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCgtgt > 2:5181/1‑115 (MQ=255) g‑cgcCATCTGCCTTCAATCCGATGATTGAGCAGATTTTTGGTGATCCATCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCTATAGGCGTGTGGATGGTAACGCGTCTAGAAGATGCCTAGCGCGtttt < 6:84358/149‑1 (MQ=255) g‑cgcCATCGGACTTCAATCCGATGAGTGTGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGtttt < 2:256406/149‑1 (MQ=255) g‑cgcCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGtttt < 6:49133/149‑1 (MQ=255) g‑cgcCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGtttt > 5:2324/1‑149 (MQ=255) tcATCGGATTGAAGTCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTg > 6:226524/2‑147 (MQ=255) tcATCGGATTGAAGTCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTg < 5:226524/146‑1 (MQ=255) cATCGGATTGAAGTCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGc > 6:180253/1‑149 (MQ=255) cATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGc > 8:48463/1‑149 (MQ=255) cGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAg > 6:16781/1‑149 (MQ=255) gACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTg > 4:152040/1‑149 (MQ=255) | GGCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGT‑GCCTGAGGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAG‑CGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGT‑CTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTG > NC_002947/2211758‑2212050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |