Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,230,781 T→C 8.9% intergenic (+109/‑3) PP_1968 → / → PP_1969 TetR family transcriptional regulator/molybdenum cofactor biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,230,7810TC 8.9% 184.7 / 6.5 67intergenic (+109/‑3)PP_1968/PP_1969TetR family transcriptional regulator/molybdenum cofactor biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base C (3/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACAAGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACGG  >  NC_002947/2230634‑2230923
                                                                                                                                                   |                                                                                                                                              
ggCAACAAGCGCAAGCGCGACCCGGAACCCTCGAACTGAGAATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGACCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCCCATTGGGGATTGCAAAAACCTGAtt                                                                                                                                               >  6:164727/1‑149 (MQ=255)
   aacaaGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTAt                                                                                                                                            <  6:271600/149‑1 (MQ=255)
          gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                     >  6:122993/1‑149 (MQ=255)
          gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                     >  2:222955/1‑149 (MQ=255)
          gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                     >  4:23047/1‑149 (MQ=255)
            aagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGt                                                                                                                                   >  3:188171/1‑149 (MQ=255)
             agcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGtt                                                                                                                                  <  8:205705/149‑1 (MQ=255)
              gcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGttt                                                                                                                                 >  2:88836/1‑149 (MQ=255)
                gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                               <  1:222955/149‑2 (MQ=255)
                gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                               <  3:23047/149‑2 (MQ=255)
                gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                               <  5:122993/149‑2 (MQ=255)
                   aCCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCTGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCg                                                                                                                            <  3:84543/149‑1 (MQ=255)
                          aCCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCtt                                                                                                                     <  5:206079/149‑1 (MQ=255)
                           cccTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTc                                                                                                                    <  8:234698/149‑1 (MQ=255)
                            ccTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCa                                                                                                                   >  5:187388/1‑149 (MQ=255)
                             cTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCAt                                                                                                                  <  4:188171/149‑1 (MQ=255)
                             cTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCAt                                                                                                                  <  8:155875/149‑1 (MQ=255)
                              tCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATc                                                                                                                 >  2:242620/1‑149 (MQ=255)
                                gAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATc                                                                                                                 <  2:306466/147‑1 (MQ=255)
                                   cTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGc                                                                                                            <  2:71860/149‑1 (MQ=255)
                                      aGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCg                                                                                                          >  2:238451/1‑148 (MQ=255)
                                       gCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                        <  4:348723/149‑1 (MQ=255)
                                       gCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                        <  1:88836/149‑1 (MQ=255)
                                           ttAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGtca                                                                                                    >  1:189236/1‑149 (MQ=255)
                                                gAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGtcattcat                                                                                               <  1:157540/149‑1 (MQ=255)
                                                    cGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGAtt                                                                                           <  6:187388/149‑1 (MQ=255)
                                                      gCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGt                                                                                         <  1:242620/149‑1 (MQ=255)
                                                         tcgtGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                        >  6:139720/1‑131 (MQ=255)
                                                         tcgtGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                        <  5:139720/131‑1 (MQ=255)
                                                           gtGAGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCAATGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATc                                                                                    >  6:170219/1‑149 (MQ=255)
                                                            tGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCg                                                                                   >  8:54611/1‑149 (MQ=255)
                                                               gTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTc                                                                                 >  1:313891/1‑148 (MQ=255)
                                                                ttCGCTGCGCGGCATTGCATCCCGAATCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCaa                                                                               <  7:233076/149‑1 (MQ=255)
                                                                  cGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAag                                                                              <  2:189236/148‑1 (MQ=255)
                                                                   gCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGa                                                                                      <  7:150344/139‑1 (MQ=255)
                                                                   gCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGa                                                                                      >  8:150344/1‑139 (MQ=255)
                                                                     tGCGCGGCAGTGCAGCCCGAAGCGTTTGGCAATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAaggcag                                                                          >  1:30132/1‑149 (MQ=255)
                                                                            cAGTGCAGCCCGAAGTGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCttt                                                                   >  8:258195/1‑149 (MQ=255)
                                                                             aGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTc                                                                  <  7:194155/149‑1 (MQ=255)
                                                                                  aGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAAc                                                             <  1:257954/149‑1 (MQ=255)
                                                                                       gAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGC‑AAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                       >  7:285532/1‑149 (MQ=255)
                                                                                               tgtcTATCGTTTGGAAATTCGGGATTGCGCATTGGGGCTTGCAAAAACGTGATTTATGTGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCTTTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGc                                                 <  5:164727/145‑1 (MQ=255)
                                                                                               tGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGc                                                 <  1:238451/148‑1 (MQ=255)
                                                                                                 gCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCt                                               >  3:152259/1‑148 (MQ=255)
                                                                                                     tCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGa                                             <  2:30132/146‑1 (MQ=255)
                                                                                                     tCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGAc                                            <  2:313891/147‑1 (MQ=255)
                                                                                                       gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                       >  8:334502/1‑134 (MQ=255)
                                                                                                       gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                       <  7:334502/134‑1 (MQ=255)
                                                                                                       gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGct                                        >  6:103142/1‑149 (MQ=255)
                                                                                                           ggAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgcct                                    >  5:73384/1‑149 (MQ=255)
                                                                                                               aTTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCaa                                <  5:170219/149‑1 (MQ=255)
                                                                                                                 tCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAAc                               >  5:62835/1‑148 (MQ=255)
                                                                                                                  cGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACt                              <  7:258195/148‑1 (MQ=255)
                                                                                                                        tGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGGGTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGCTCGTCAAGGAAGGCGATTTCGCAACCTGAGCGTCCGACGGTCGGCTGCCTGCAACTCCGACTg                       >  8:240417/1‑149 (MQ=255)
                                                                                                                             aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACgg                                          <  4:321972/125‑1 (MQ=255)
                                                                                                                             aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACgg                                          >  3:321972/1‑125 (MQ=255)
                                                                                                                             aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgc                                      <  4:10618/129‑1 (MQ=255)
                                                                                                                             aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgc                                      >  3:10618/1‑129 (MQ=255)
                                                                                                                             aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTAcc                   >  8:236565/1‑148 (MQ=255)
                                                                                                                                       gCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTAc         <  7:236565/148‑1 (MQ=255)
                                                                                                                                       gCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTAc         >  2:277813/1‑148 (MQ=255)
                                                                                                                                        cAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCt       <  6:204888/149‑1 (MQ=255)
                                                                                                                                        cAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCt       >  5:204888/1‑149 (MQ=255)
                                                                                                                                         aaaaaCCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTg      >  7:20016/1‑149 (MQ=255)
                                                                                                                                          aaaaCCTGATTTAAGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGa     >  7:246150/1‑149 (MQ=255)
                                                                                                                                            aaCCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACg   <  6:73384/149‑1 (MQ=255)
                                                                                                                                               cTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACgg  >  6:26487/1‑147 (MQ=255)
                                                                                                                                               cTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACgg  <  5:26487/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GGCAACAAGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACGG  >  NC_002947/2230634‑2230923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑