Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,230,783 T→C 8.9% intergenic (+111/‑1) PP_1968 → / → PP_1969 TetR family transcriptional regulator/molybdenum cofactor biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,230,7830TC 8.9% 184.0 / 6.9 67intergenic (+111/‑1)PP_1968/PP_1969TetR family transcriptional regulator/molybdenum cofactor biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base C (3/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAAGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACGGAAAGCGG  >  NC_002947/2230637‑2230930
                                                                                                                                                  |                                                                                                                                                   
aacaaGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTAt                                                                                                                                                   <  6:271600/149‑1 (MQ=255)
       gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                            >  4:23047/1‑149 (MQ=255)
       gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                            >  6:122993/1‑149 (MQ=255)
       gcaagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAg                                                                                                                                            >  2:222955/1‑149 (MQ=255)
         aagcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGt                                                                                                                                          >  3:188171/1‑149 (MQ=255)
          agcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGtt                                                                                                                                         <  8:205705/149‑1 (MQ=255)
           gcgcGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGttt                                                                                                                                        >  2:88836/1‑149 (MQ=255)
             gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                                      <  5:122993/149‑2 (MQ=255)
             gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                                      <  1:222955/149‑2 (MQ=255)
             gcgACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGACTCATAAATCAGGttttt                                                                                                                                      <  3:23047/149‑2 (MQ=255)
                aCCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCTGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCg                                                                                                                                   <  3:84543/149‑1 (MQ=255)
                       aCCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCtt                                                                                                                            <  5:206079/149‑1 (MQ=255)
                        cccTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTc                                                                                                                           <  8:234698/149‑1 (MQ=255)
                         ccTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCa                                                                                                                          >  5:187388/1‑149 (MQ=255)
                          cTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCAt                                                                                                                         <  4:188171/149‑1 (MQ=255)
                          cTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCAt                                                                                                                         <  8:155875/149‑1 (MQ=255)
                           tCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATc                                                                                                                        >  2:242620/1‑149 (MQ=255)
                             gAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATc                                                                                                                        <  2:306466/147‑1 (MQ=255)
                                cTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGc                                                                                                                   <  2:71860/149‑1 (MQ=255)
                                   aGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCg                                                                                                                 >  2:238451/1‑148 (MQ=255)
                                    gCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                               <  4:348723/149‑1 (MQ=255)
                                    gCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                               <  1:88836/149‑1 (MQ=255)
                                        ttAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGtca                                                                                                           >  1:189236/1‑149 (MQ=255)
                                             gAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGtcattcat                                                                                                      <  1:157540/149‑1 (MQ=255)
                                                 cGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGAtt                                                                                                  <  6:187388/149‑1 (MQ=255)
                                                   gCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGt                                                                                                <  1:242620/149‑1 (MQ=255)
                                                      tcgtGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                               >  6:139720/1‑131 (MQ=255)
                                                      tcgtGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGa                                                                                                               <  5:139720/131‑1 (MQ=255)
                                                        gtGAGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCAATGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATc                                                                                           >  6:170219/1‑149 (MQ=255)
                                                         tGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCg                                                                                          >  8:54611/1‑149 (MQ=255)
                                                            gTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTc                                                                                        >  1:313891/1‑148 (MQ=255)
                                                             ttCGCTGCGCGGCATTGCATCCCGAATCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCaa                                                                                      <  7:233076/149‑1 (MQ=255)
                                                               cGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAag                                                                                     <  2:189236/148‑1 (MQ=255)
                                                                gCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGa                                                                                             <  7:150344/139‑1 (MQ=255)
                                                                gCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGa                                                                                             >  8:150344/1‑139 (MQ=255)
                                                                  tGCGCGGCAGTGCAGCCCGAAGCGTTTGGCAATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAaggcag                                                                                 >  1:30132/1‑149 (MQ=255)
                                                                         cAGTGCAGCCCGAAGTGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCttt                                                                          >  8:258195/1‑149 (MQ=255)
                                                                          aGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTc                                                                         <  7:194155/149‑1 (MQ=255)
                                                                               aGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAAc                                                                    <  1:257954/149‑1 (MQ=255)
                                                                                    gAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGC‑AAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                              >  7:285532/1‑149 (MQ=255)
                                                                                            tgtcTATCGTTTGGAAATTCGGGATTGCGCATTGGGGCTTGCAAAAACGTGATTTATGTGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCTTTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGc                                                        <  5:164727/145‑1 (MQ=255)
                                                                                            tGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGc                                                        <  1:238451/148‑1 (MQ=255)
                                                                                              gCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCt                                                      >  3:152259/1‑148 (MQ=255)
                                                                                                  tCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGa                                                    <  2:30132/146‑1 (MQ=255)
                                                                                                  tCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGAc                                                   <  2:313891/147‑1 (MQ=255)
                                                                                                    gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                              <  7:334502/134‑1 (MQ=255)
                                                                                                    gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTgcgc                                                              >  8:334502/1‑134 (MQ=255)
                                                                                                    gTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGct                                               >  6:103142/1‑149 (MQ=255)
                                                                                                        ggAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgcct                                           >  5:73384/1‑149 (MQ=255)
                                                                                                            aTTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCaa                                       <  5:170219/149‑1 (MQ=255)
                                                                                                              tCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAAc                                      >  5:62835/1‑148 (MQ=255)
                                                                                                               cGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACt                                     <  7:258195/148‑1 (MQ=255)
                                                                                                                     tGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGGGTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGCTCGTCAAGGAAGGCGATTTCGCAACCTGAGCGTCCGACGGTCGGCTGCCTGCAACTCCGACTg                              >  8:240417/1‑149 (MQ=255)
                                                                                                                          aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACgg                                                 >  3:321972/1‑125 (MQ=255)
                                                                                                                          aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACgg                                                 <  4:321972/125‑1 (MQ=255)
                                                                                                                          aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgc                                             <  4:10618/129‑1 (MQ=255)
                                                                                                                          aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGctgc                                             >  3:10618/1‑129 (MQ=255)
                                                                                                                          aTTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTAcc                          >  8:236565/1‑148 (MQ=255)
                                                                                                                                    gCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTAc                <  7:236565/148‑1 (MQ=255)
                                                                                                                                    gCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTAc                >  2:277813/1‑148 (MQ=255)
                                                                                                                                     cAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCt              <  6:204888/149‑1 (MQ=255)
                                                                                                                                     cAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCt              >  5:204888/1‑149 (MQ=255)
                                                                                                                                      aaaaaCCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTg             >  7:20016/1‑149 (MQ=255)
                                                                                                                                       aaaaCCTGATTTAAGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGa            >  7:246150/1‑149 (MQ=255)
                                                                                                                                         aaCCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACg          <  6:73384/149‑1 (MQ=255)
                                                                                                                                            cTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACgg         >  6:26487/1‑147 (MQ=255)
                                                                                                                                            cTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACgg         <  5:26487/147‑1 (MQ=255)
                                                                                                                                                 ttATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGACGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCCCTTTAGCAACCTGCGCGTCAGCATGACTGCTGCCTGCAACTAAGCCTGTACCTACTGCGTACCTGACGGAAAGCgg  >  3:344571/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACAAGCGCAAGCGCGACCCGGAACCCTCGAACTGAGCATTTAGCGAAACGGCGTCGTGCGTTCGCTGCGCGGCAGTGCAGCCCGAAGCGTTTGGCTATCGTTTGGAAATTCGGCATTGCGCATTGGGGCTTGCAAAAACCTGATTTATGAGTCAGGTTTTGCCGGCCCCTTCATCCATGCCGGAGTCATTCATGATTGTCGATCGTCAAGGCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGCGTACCTGACGGAAAGCGG  >  NC_002947/2230637‑2230930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑