| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,241,306 | A→G | 11.9% | intergenic (+53/‑228) | gltX → / → PP_t25 | glutamate‑‑tRNA ligase/tRNA‑Ala |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,241,306 | 0 | A | G | 11.9% | 86.7 / 3.7 | 38 | intergenic (+53/‑228) | gltX/PP_t25 | glutamate‑‑tRNA ligase/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base A (14/18); new base G (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
GCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACT > NC_002947/2241160‑2241452 | gcgcTCCCGCCTGCGCCAGGCTCTTTACCTGCTGGGTGGGTTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTaaa < 8:28321/149‑1 (MQ=255) ccGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAat < 7:234743/149‑1 (MQ=255) cGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAata > 5:208003/1‑149 (MQ=255) gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGGAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATACAATGGTAAATTCAata > 2:73433/1‑148 (MQ=255) cTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCg < 6:208003/149‑1 (MQ=255) cgcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCa < 7:109135/148‑1 (MQ=255) cgcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCa < 5:104561/148‑1 (MQ=255) gcCAGGCTCTGGACCTGCTGTGTGGGTTGTAGAAGAAAGAAAACAAAGAGTTGGAAATTCTGTTGGCTTCCATAGCATGATTCAGCTTTGCACCTAAACAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCAtt < 5:188790/149‑1 (MQ=255) tctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc > 3:295712/1‑149 (MQ=255) tGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATTTc < 2:306092/135‑1 (MQ=255) gACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCAc < 4:339464/147‑1 (MQ=25) gACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGc < 5:111261/149‑1 (MQ=25) ctggggtaaacGCTGTTGGCTTCCATCGCCTGATTCCGCTTTCCACCAACCCTGTGGCCGC‑GGGCGGGCCCGCTAAGGAAATGGTAAATTCAATATCCCATGCCCCGCCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCgg < 2:190359/142‑1 (MQ=255) aaaaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAggg < 7:117956/149‑1 (MQ=255) tGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGGGTGGCCCCGCATTGAATGGGTAATTTAATATAGGATTAGCCGGGCCGCCCCCGCCCCCAGGGCCACAAAGCCCCAAAAAAACGGGGGAgggggggga > 6:2478/1‑146 (MQ=255) ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCAc < 7:337167/96‑1 (MQ=255) ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCAc > 8:337167/1‑96 (MQ=255) ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTAAATATCGCATTCGCCGGCACGCCCGCGCCCCCAGGCGCACTAAGCCCCAAAAAACCGGGGTAGGGCGGGAAGt > 8:90693/1‑149 (MQ=255) ggCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGGCCCCGAATTGAAATGTTAATTGAATATAGCATTGGCCCGCCCGCCCCCACCCCCAGGCCCACGAAGCCCCAAAAAACCCGGGGTGGGCGGTTAGTgg > 4:97725/1‑148 (MQ=255) ggCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGt < 3:97725/147‑1 (MQ=255) gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATTGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGa < 4:295712/148‑1 (MQ=255) cTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt < 8:76677/149‑1 (MQ=255) ttCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTTCCCGCGCATGCAATGGTAAATTAAATATAGCATTCGCCGGCACGCCCCCTCCCACAGGCCCACTAAGCCCCAAAAAACCGGGGGAGGGCGGTAAGTGATTg > 5:121937/1‑149 (MQ=255) cATCGCCTCATACAGTTTTGCACCTAACCTGGGGGAGC‑GGGGGTGCCCGCCAATGAAATTGAAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGtt < 2:340985/148‑1 (MQ=255) tCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATACAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATc < 1:73433/149‑1 (MQ=255) ccTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTAAATATAGCATTAGCCGGCACGCCCGCTGCCCCAGGCCAACTAAGCCCCAAAAAACCCGGGTAGGGCGGTAAGTGATTGTTTTCTGTGTAAAAATTTTTGAatt > 6:185734/1‑148 (MQ=255) aaCCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAAATGTAAATTCAATTAAGCATTCGCCGGCCCGCCCGCTGCCACAGGGCAACTAAGCCCCAAAAAAACGGGGTAGGGGGGTAAGTGATTGTTATATGTGAAAAAATTTTTGAATtttt > 6:359389/1‑149 (MQ=255) cAGTGGGAGCGGGGCGTGCCCCCGAATGCAATGGTAAATTAAAAATAGCAATACCCGGCCCCCCCCCACCCCCAGGCCAACTAAGCCCCAAAAAACCGGGGGAGGGCGGTTATTGATTGTTTTAAGTGTAAAAAttttt > 4:289364/1‑139 (MQ=255) cAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt < 3:289364/138‑1 (MQ=255) tGGGCGC‑GGGCGTGCCCGCTAATGCAATTGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGaaa < 3:201640/149‑1 (MQ=255) cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc > 1:85190/3‑149 (MQ=25) cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc > 4:42326/3‑149 (MQ=25) cgcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt > 3:186059/1‑90 (MQ=255) cgcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt < 4:186059/90‑1 (MQ=255) aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc > 4:182389/1‑148 (MQ=255) tGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa > 2:98530/1‑149 (MQ=255) gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc < 6:121937/148‑1 (MQ=255) gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACt > 7:112370/1‑149 (MQ=255) tAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGATTTTTTTTGAAAATTAGTTTGACAGACCTGCAATCCGAACt > 8:244006/1‑148 (MQ=255) | GCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACT > NC_002947/2241160‑2241452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |