Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,241,326 C→G 9.8% intergenic (+73/‑208) gltX → / → PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,241,3260CG 9.8% 115.7 / 4.4 41intergenic (+73/‑208)gltX/PP_t25glutamate‑‑tRNA ligase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (20/17);  new base G (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

TCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAG  >  NC_002947/2241181‑2241473
                                                                                                                                                  |                                                                                                                                                   
tctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                                                  >  3:295712/1‑149 (MQ=255)
    gACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCAc                                                                                                                                                <  4:339464/147‑1 (MQ=25)
    gACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGc                                                                                                                                              <  5:111261/149‑1 (MQ=25)
                                        ctggggtaaacGCTGTTGGCTTCCATCGCCTGATTCCGCTTTCCACCAACCCTGTGGCCGC‑GGGCGGGCCCGCTAAGGAAATGGTAAATTCAATATCCCATGCCCCGCCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCgg                                                                                                          <  2:190359/142‑1 (MQ=255)
                                               aaaaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAggg                                                                                                   <  7:117956/149‑1 (MQ=255)
                                                     tGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGGGTGGCCCCGCATTGAATGGGTAATTTAATATAGGATTAGCCGGGCCGCCCCCGCCCCCAGGGCCACAAAGCCCCAAAAAAACGGGGGAgggggggga                                                                                             >  6:2478/1‑146 (MQ=255)
                                                       ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCAc                                                                                                                                                >  8:337167/1‑96 (MQ=255)
                                                       ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCAc                                                                                                                                                <  7:337167/96‑1 (MQ=255)
                                                       ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTAAATATCGCATTCGCCGGCACGCCCGCGCCCCCAGGCGCACTAAGCCCCAAAAAACCGGGGTAGGGCGGGAAGt                                                                                           >  8:90693/1‑149 (MQ=255)
                                                         ggCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGGCCCCGAATTGAAATGTTAATTGAATATAGCATTGGCCCGCCCGCCCCCACCCCCAGGCCCACGAAGCCCCAAAAAACCCGGGGTGGGCGGTTAGTgg                                                                                         >  4:97725/1‑148 (MQ=255)
                                                         ggCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGt                                                                                           <  3:97725/147‑1 (MQ=255)
                                                          gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATTGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGa                                                                                         <  4:295712/148‑1 (MQ=255)
                                                           cTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt                                                                                       <  8:76677/149‑1 (MQ=255)
                                                            ttCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTTCCCGCGCATGCAATGGTAAATTAAATATAGCATTCGCCGGCACGCCCCCTCCCACAGGCCCACTAAGCCCCAAAAAACCGGGGGAGGGCGGTAAGTGATTg                                                                                      >  5:121937/1‑149 (MQ=255)
                                                               cATCGCCTCATACAGTTTTGCACCTAACCTGGGGGAGC‑GGGGGTGCCCGCCAATGAAATTGAAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGtt                                                                                    <  2:340985/148‑1 (MQ=255)
                                                                 tCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATACAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATc                                                                                 <  1:73433/149‑1 (MQ=255)
                                                                                     ccTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTAAATATAGCATTAGCCGGCACGCCCGCTGCCCCAGGCCAACTAAGCCCCAAAAAACCCGGGTAGGGCGGTAAGTGATTGTTTTCTGTGTAAAAATTTTTGAatt                                                             >  6:185734/1‑148 (MQ=255)
                                                                                        aaCCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAAATGTAAATTCAATTAAGCATTCGCCGGCCCGCCCGCTGCCACAGGGCAACTAAGCCCCAAAAAAACGGGGTAGGGGGGTAAGTGATTGTTATATGTGAAAAAATTTTTGAATtttt                                                          >  6:359389/1‑149 (MQ=255)
                                                                                           cAGTGGGAGCGGGGCGTGCCCCCGAATGCAATGGTAAATTAAAAATAGCAATACCCGGCCCCCCCCCACCCCCAGGCCAACTAAGCCCCAAAAAACCGGGGGAGGGCGGTTATTGATTGTTTTAAGTGTAAAAAttttt                                                                  >  4:289364/1‑139 (MQ=255)
                                                                                           cAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAttttt                                                                  <  3:289364/138‑1 (MQ=255)
                                                                                              tGGGCGC‑GGGCGTGCCCGCTAATGCAATTGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGaaa                                                    <  3:201640/149‑1 (MQ=255)
                                                                                                              cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                                     >  4:42326/3‑149 (MQ=25)
                                                                                                              cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                                     >  1:85190/3‑149 (MQ=25)
                                                                                                               cgcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt                                                                                               <  4:186059/90‑1 (MQ=255)
                                                                                                               cgcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt                                                                                               >  3:186059/1‑90 (MQ=255)
                                                                                                                        aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc                            >  4:182389/1‑148 (MQ=255)
                                                                                                                          tGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa                         >  2:98530/1‑149 (MQ=255)
                                                                                                                            gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc                        <  6:121937/148‑1 (MQ=255)
                                                                                                                            gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACt                       >  7:112370/1‑149 (MQ=255)
                                                                                                                             tAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGATTTTTTTTGAAAATTAGTTTGACAGACCTGCAATCCGAACt                       >  8:244006/1‑148 (MQ=255)
                                                                                                                               aaTTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTaa                    <  5:2478/149‑1 (MQ=255)
                                                                                                                                  tCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAatat                 >  2:294719/1‑149 (MQ=255)
                                                                                                                                    aaTATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGa                                                                <  4:313587/100‑1 (MQ=255)
                                                                                                                                    aaTATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATgc               <  1:98530/149‑1 (MQ=255)
                                                                                                                                     atatCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAata                  <  3:118804/145‑1 (MQ=255)
                                                                                                                                     atatCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAata                  >  4:118804/1‑145 (MQ=255)
                                                                                                                                     atatCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATgcg              >  3:136306/1‑149 (MQ=255)
                                                                                                                                       atCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGGAAGTGCTTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAATTTAGTTTGAGAGCCCTGCAGTCCGAACTTATTATGCGcc            >  2:223195/1‑149 (MQ=255)
                                                                                                                                        tCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGccc           >  8:276308/1‑149 (MQ=255)
                                                                                                                                        tCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGccc           >  3:71958/1‑149 (MQ=255)
                                                                                                                                            atgcgccgcCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTc       <  3:214509/146‑1 (MQ=255)
                                                                                                                                            agtCGCCGGCAGGCCCGCTCACACAGGCAAACTAAGACCCATAAAACCGGGGTAGGTCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGTACTTAATATGCGCCCCGTc       <  3:11764/147‑1 (MQ=255)
                                                                                                                                                 ccGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGGCAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAg  >  2:73470/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGC‑GGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAG  >  NC_002947/2241181‑2241473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑