Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,284,904	C→T	5.8%	R53C (CGC→TGC)	PP_2013 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,284,904	0	C	T	5.8%	203.4 / 2.0	69	R53C (CGC→TGC)	PP_2013	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAAGTTTTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTATGTCCGC  >  NC_002947/2284762‑2285050                                                                                                                                               |                                                                                                                                                   gcgAAGTTTTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGc                                                                                                                                              >  5:156832/1‑149 (MQ=255)   gAAGTTTTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCa                                                                                                                                            >  7:92000/1‑149 (MQ=255)   gAAGTTTTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCa                                                                                                                                            >  5:341267/1‑149 (MQ=255)   gAAGTATTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCa                                                                                                                                            >  8:30458/1‑149 (MQ=255)              atcatcGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGTCGCTGTTCCTTGGCGATATCGTCGACCGCTGGCCACGCATTCGCGa                                                                                                                                 <  2:56592/149‑1 (MQ=255)              atcatcGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGa                                                                                                                                 <  5:138935/149‑1 (MQ=255)               tcatcGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAg                                                                                                                                <  6:156832/149‑1 (MQ=255)                catcGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGTGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAgg                                                                                                                               >  2:274877/1‑149 (MQ=255)                      gtcagTGCACAGCCGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACTGCGGGCCACGCATTCGCGAGGCGCTg                                                                                                                          >  6:224139/5‑148 (MQ=255)                      gtcagTGCACAGCCGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACTGCGGGCCACGCATTCGCGAGGCGCTg                                                                                                                          >  6:218908/5‑148 (MQ=255)                            gCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCg                                                                                                                   >  2:326976/1‑149 (MQ=255)                                   tgtgCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGTCCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGa                                                                                                            <  7:30458/149‑1 (MQ=255)                                      gCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACAt                                                                                                         >  1:277558/1‑149 (MQ=255)                                        aCTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGAGCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATgg                                                                                                       <  5:357955/149‑1 (MQ=255)                                         cTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCAATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGc                                                                                                                         >  2:69872/1‑130 (MQ=255)                                         cTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCAATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGc                                                                                                                         <  1:69872/130‑1 (MQ=255)                                           gACCCTTGAACGCCTGCTCGACGCCCTCGCATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTc                                                                                                     >  8:203780/1‑148 (MQ=255)                                              ccTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAg                                                                                                 >  5:370930/1‑149 (MQ=255)                                               cTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGATATGGTCGAAGc                                                                                                >  4:74891/1‑149 (MQ=255)                                               cTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGc                                                                                                >  4:45035/1‑149 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACcgc                                                                                                                                                  >  6:303778/1‑98 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACcgc                                                                                                                                                  <  5:303778/98‑1 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGca                                                                                                                        >  7:330568/1‑124 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGca                                                                                                                        <  8:330568/124‑1 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGcc                                                                                               >  4:280544/1‑149 (MQ=255)                                                ttGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGcc                                                                                               >  6:136960/1‑149 (MQ=255)                                                  gAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCg                                                                                              <  1:307154/148‑1 (MQ=255)                                                         tGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCg                                                                                      >  1:174762/1‑149 (MQ=255)                                                              gACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCATGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCt                                                                                  >  5:297635/1‑148 (MQ=255)                                                              gACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTg                                                                                 >  7:47544/1‑149 (MQ=255)                                                               aCGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTg                                                                                 >  5:74227/1‑148 (MQ=255)                                                                   ccTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGcatca                                                                            >  7:150763/1‑149 (MQ=255)                                                                   ccTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGcatca                                                                            >  3:116206/1‑149 (MQ=255)                                                                   ccTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGcatca                                                                            >  5:205923/1‑149 (MQ=255)                                                                     tCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATg                                                                          >  4:259435/1‑149 (MQ=255)                                                                       ggATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATgg                                                                         <  6:74227/148‑1 (MQ=255)                                                                       ggATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATgg                                                                         <  2:277558/148‑1 (MQ=255)                                                                       ggATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATgg                                                                         <  7:98507/148‑1 (MQ=255)                                                                             aaGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCa                                                                  >  1:309437/1‑149 (MQ=255)                                                                              aGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCgg                                                                                             >  5:182585/1‑121 (MQ=255)                                                                              aGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCgg                                                                                             <  6:182585/121‑1 (MQ=255)                                                                              aGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCAc                                                                 <  7:203780/149‑1 (MQ=255)                                                                                 gcgTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGaa                                                              <  5:37594/149‑1 (MQ=255)                                                                                  cgTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACTGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAAt                                                             <  5:218908/149‑1 (MQ=255)                                                                                  cgTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACTGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAAt                                                             <  5:224139/149‑1 (MQ=255)                                                                                    tGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATAc                                                           <  5:136960/149‑1 (MQ=255)                                                                                      gCCGGCGTGTGGCTCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACaa                                                         <  7:79774/149‑1 (MQ=255)                                                                                        cGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATg                                                       <  6:341267/149‑1 (MQ=255)                                                                                           cGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGccc                                                    <  6:205923/149‑1 (MQ=255)                                                                                             tgtgGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCctg                                                  <  5:109340/149‑1 (MQ=255)                                                                                             tgtgGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCctg                                                  <  7:212833/149‑1 (MQ=255)                                                                                               tgGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACGGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCctggg                                                <  3:20004/149‑1 (MQ=255)                                                                                                 gcgcCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCctgggct                                              <  3:45035/149‑1 (MQ=255)                                                                                                   gcCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCctgggctgg                                            <  8:92000/149‑1 (MQ=255)                                                                                                            gACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCg                                   <  8:47544/149‑1 (MQ=255)                                                                                                                 cAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCt                              >  1:63973/1‑149 (MQ=255)                                                                                                                     cgcTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGATATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCt                          <  3:74891/149‑1 (MQ=255)                                                                                                                        tGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGTGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCAGGAGCTGCCTGGc                       >  8:245417/1‑149 (MQ=255)                                                                                                                        tGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTgg                        >  5:122349/1‑148 (MQ=255)                                                                                                                            ccTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCt                          <  3:49643/142‑1 (MQ=255)                                                                                                                            ccTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCt                          >  4:49643/1‑142 (MQ=255)                                                                                                                            ccTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCg                   >  6:325903/1‑149 (MQ=255)                                                                                                                              ttGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCgg                  >  5:133923/1‑148 (MQ=255)                                                                                                                                 gCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCaa               <  2:174762/148‑1 (MQ=255)                                                                                                                                 gCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCaa               <  3:259435/148‑1 (MQ=255)                                                                                                                                   gATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGc            <  1:326976/149‑1 (MQ=255)                                                                                                                                     tatCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCAGCGCTGCCTGGCAGCGGCAAGGCCt          <  6:370930/149‑1 (MQ=255)                                                                                                                                       tcgtcgACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTAt        <  3:1533/149‑1 (MQ=255)                                                                                                                                       tcgtcgACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTAt        <  3:280544/149‑1 (MQ=255)                                                                                                                                          tcgACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTATGTc     >  2:123649/1‑149 (MQ=255)                                                                                                                                             aCCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTATGTCcgc  <  5:325903/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GCGAAGTTTTGACATCATCGGCGACGTGCACGGCTGTGCACTGACCCTTGAACGCCTGCTCGACGCCCTCGGATACAAGCGCGTGGCCGGCGTGTGGCGCCACCCGCGACGTCAGGCGCTGTTCCTTGGCGATATCGTCGACCGCGGGCCACGCATTCGCGAGGCGCTGCACATCGTCCACGACATGGTCGAAGCCGGCCAGGCGTTCTGCATCATGGGCAACCACGAATACAATGCCCTGGGCTGGGTCACCCCGGCGCTGCCTGGCAGCGGCAAGGCCTATGTCCGC  >  NC_002947/2284762‑2285050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑