Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,320,200	C→G	6.0%	L405V (CTG→GTG)	PP_2040 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,320,200	0	C	G	6.0%	212.7 / 4.2	67	L405V (CTG→GTG)	PP_2040	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (34/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

ATCGGCAACGGTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTT  >  NC_002947/2320059‑2320343                                                                                                                                               |                                                                                                                                                aTCGGCAACGGTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCg                                                                                                                                           >  6:162829/1‑148 (MQ=255)  tCGGCAACGGTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCggg                                                                                                                                         >  5:194363/1‑149 (MQ=255)    ggCAACGGTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCt                                                                                                                                       >  4:103274/1‑149 (MQ=255)           gTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCtg                                                                                                                                 >  3:69926/1‑148 (MQ=255)            tATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCtgga                                                                                                                               <  6:194363/149‑1 (MQ=255)              tCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATTCGCCGGGCTGATCGGGCTGGCCtggat                                                                                                                              <  1:4249/149‑1 (MQ=255)              tCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCtggat                                                                                                                              <  7:4703/148‑1 (MQ=255)                           cGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGaaaa                                                                                                                >  1:215687/1‑149 (MQ=255)                             ggCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAAcc                                                                                                              <  4:69926/149‑1 (MQ=255)                               cTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCg                                                                                                            >  2:198538/1‑149 (MQ=255)                               cTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCg                                                                                                            <  3:173157/149‑1 (MQ=255)                                tGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCg                                                                                                                                           <  5:172437/117‑1 (MQ=255)                                tGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCg                                                                                                                                           >  6:172437/1‑117 (MQ=255)                                  tCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCgg                                                                                                           <  6:17793/147‑1 (MQ=255)                                     gCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACg                                                                                                      >  7:117233/1‑149 (MQ=255)                                       aCCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTc                                                                                                    >  4:149025/1‑149 (MQ=255)                                         cGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCTGCGCGGTGCTGGTGATT‑GCCGGCCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTg                                                                                                  >  3:222672/1‑149 (MQ=255)                                          ggtggtCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTgg                                                                                                 >  2:337770/1‑149 (MQ=255)                                           gtggtCACCGGTTTGCTGGTCCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGt                                                                                                >  8:24403/1‑149 (MQ=255)                                             ggtCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTg                                                                                               >  3:86300/1‑148 (MQ=255)                                               tCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAg                                                                                            <  3:103030/149‑1 (MQ=255)                                               tCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAg                                                                                            <  5:156124/149‑1 (MQ=255)                                                cACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGGTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAgg                                                                                           >  6:120300/1‑149 (MQ=255)                                                cACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGGTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAgg                                                                                           >  6:115066/1‑149 (MQ=255)                                                cACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAgg                                                                                           >  6:42220/1‑149 (MQ=255)                                                 aCCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGt                                                                                          >  1:182671/1‑149 (MQ=255)                                                   cGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTgg                                                                                        >  1:325949/1‑149 (MQ=255)                                                    ggTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTggg                                                                                       <  7:142948/149‑1 (MQ=255)                                                        tGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCAc                                                                                    <  1:367905/148‑1 (MQ=255)                                                         gCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACgg                                                                                  >  5:72487/1‑149 (MQ=255)                                                           tGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCaggcgc                                                                                >  4:236550/1‑144 (MQ=255)                                                               gCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTgcgc                                                                            <  3:103274/149‑1 (MQ=255)                                                                cAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGcc                                                                           <  3:149025/149‑1 (MQ=255)                                                                    gcgcCGGGGCCGGGCACACGGGAGTGTTTGGCCAGGGTTTTCTTGTGTGCGGCGCTGTGCTGGTGATT‑GCCGGGCTGATCTGGCTGGCCTGGATGGACCCGCAGAACACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGc                                                                        <  4:222672/148‑1 (MQ=255)                                                                     cgcCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGACTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGccc                                                                      <  5:162829/149‑1 (MQ=255)                                                                      gccggggccgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGccc                                                                      >  5:3872/1‑148 (MQ=255)                                                                       ccggggccgggCACACGGCAGGGTTTGGCCTGGGTTTTCTGGTGTGCGGCGCGGTACTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGccc                                                                      >  1:33247/1‑147 (MQ=255)                                                                         ggggccgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGGTGATCGGGCTGGCCTGGATGGACCCGCCGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGc                                                                  <  5:115066/149‑1 (MQ=255)                                                                         ggggccgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGGTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGc                                                                  <  5:120300/149‑1 (MQ=255)                                                                         ggggccgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGc                                                                  <  2:156404/149‑1 (MQ=255)                                                                         ggggccgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGc                                                                  <  6:3872/149‑1 (MQ=255)                                                                              cgggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGAt                                                             <  2:36893/149‑1 (MQ=255)                                                                               gggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATg                                                            <  7:97928/149‑1 (MQ=255)                                                                                ggCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGt                                                           <  5:42220/149‑1 (MQ=255)                                                                                 gCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGtt                                                          <  8:117233/149‑1 (MQ=255)                                                                                     acGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACt                                                      >  5:378556/1‑149 (MQ=255)                                                                                            ggTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCt                                                 >  2:154875/1‑147 (MQ=255)                                                                                            ggTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCa                                               <  6:72487/149‑1 (MQ=255)                                                                                             gTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCt                                                 >  5:67527/1‑146 (MQ=255)                                                                                                   ccAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAAGCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGctc                                        >  1:118773/1‑149 (MQ=255)                                                                                                    cAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGctct                                       >  3:164048/1‑149 (MQ=255)                                                                                                       ggTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCt                                    >  6:167889/1‑149 (MQ=255)                                                                                                         ttttCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTcc                                  >  3:208006/1‑149 (MQ=255)                                                                                                                gtgtGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAATACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAgg                            <  5:357486/148‑1 (MQ=255)                                                                                                                gtgtGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAggg                           <  7:24403/149‑1 (MQ=255)                                                                                                                gtgtGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAg                             <  3:322943/147‑1 (MQ=255)                                                                                                                agatgCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAggg                           <  1:175824/146‑1 (MQ=255)                                                                                                                 tgtgCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGc                          <  1:337770/149‑1 (MQ=255)                                                                                                                   tgCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCt                        >  8:110784/1‑149 (MQ=255)                                                                                                                      ggcgCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTcgc                     >  5:227954/1‑149 (MQ=255)                                                                                                                         gcgGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCt                  <  2:182671/149‑1 (MQ=255)                                                                                                                               cTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATc             >  5:65921/1‑148 (MQ=255)                                                                                                                               cTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATc             >  8:156086/1‑148 (MQ=255)                                                                                                                                  gTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAg          <  4:86300/148‑1 (MQ=255)                                                                                                                                   tGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAGGt        >  8:167518/1‑149 (MQ=255)                                                                                                                                         gCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCtt   >  6:178050/1‑149 (MQ=255)                                                                                                                                          ccGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCttt  >  4:163715/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                ATCGGCAACGGTATCGCCTCGTTGGCCGGGCTGTCGGCACCGGTGGTCACCGGTTTGCTGGTGCAGGGCGCCGGGGCCGGGCACACGGCAGGGTTTGGCCAGGGTTTTCTGGTGTGCGGCGCGGTGCTGGTGATT‑GCCGGGCTGATCGGGCTGGCCTGGATGGACCCGCAGAAAACCCGGCAACGTCTGGTGCAGGTGGGCACGGCTGCGCCTGCCCAAGCGTGATGTTCACTGCGCTCAGAGGCTCTGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTT  >  NC_002947/2320059‑2320343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑