Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,333,574 A→G 5.4% D169G (GAC→GGC)  fadA → 3‑ketoacyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,333,5740AG 5.4% 222.1 / 2.1 74D169G (GAC→GGC) fadA3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base G (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

CCTGGTGCAGCAGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCA  >  NC_002947/2333431‑2333721
                                                                                                                                               |                                                                                                                                                   
ccTGGTGCAGCAGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTc                                                                                                                                                 >  6:89833/1‑148 (MQ=255)
      gcagcaGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGccc                                                                                                                                          >  4:20430/1‑149 (MQ=255)
        agcagGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTg                                                                                                                                        <  3:20430/149‑1 (MQ=255)
            ggTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGt                                                                                                                                    <  3:137973/149‑1 (MQ=255)
             gTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTc                                                                                                                                   <  3:253729/149‑1 (MQ=255)
                   aaCCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCgcagca                                                                                                                             <  5:182011/149‑1 (MQ=255)
                   aaCCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCgcag                                                                                                                               >  7:219559/1‑147 (MQ=255)
                      cAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAgcg                                                                                                                          >  7:290834/1‑149 (MQ=255)
                                   aGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                             <  8:219559/149‑1 (MQ=255)
                                    ggCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                             >  8:4926/1‑148 (MQ=255)
                                     gCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                             >  2:129123/1‑147 (MQ=255)
                                       ttATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGAGGCGGCGCAgg                                                                                                         >  2:247794/1‑149 (MQ=255)
                                           gACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAggcggc                                                                                                     >  5:244888/1‑149 (MQ=255)
                                             ccccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGcggcgg                                                                                                   <  1:211684/149‑1 (MQ=255)
                                             ccccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGcggcgg                                                                                                   >  4:27190/1‑149 (MQ=255)
                                              cccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCggg                                                                                                  <  5:89833/149‑1 (MQ=255)
                                              cccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCggg                                                                                                  >  3:30924/1‑149 (MQ=255)
                                                cgcgGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgt                                                                                                >  4:174462/1‑149 (MQ=255)
                                                     tATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTc                                                                                           <  1:129123/149‑1 (MQ=255)
                                                                        tGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCgg                                                                        <  8:290834/149‑1 (MQ=255)
                                                                         gCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgtt                                                                                               <  7:256316/125‑1 (MQ=255)
                                                                         gCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgtt                                                                                               >  8:256316/1‑125 (MQ=255)
                                                                          ccTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGt                                                                       <  7:22517/148‑1 (MQ=255)
                                                                           cTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGa                                                                     >  5:170377/1‑149 (MQ=255)
                                                                              cATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcg                                                                  >  5:255439/1‑149 (MQ=255)
                                                                              cATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATc                                                                   <  3:27190/148‑1 (MQ=255)
                                                                                tGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgca                                                                <  7:268601/149‑1 (MQ=255)
                                                                                   cGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacg                                                              >  8:191981/1‑148 (MQ=255)
                                                                                   cGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacg                                                              >  1:154442/1‑148 (MQ=255)
                                                                                    gATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacgc                                                             >  6:353575/1‑148 (MQ=255)
                                                                                        ctgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaa                                                        <  3:174462/149‑1 (MQ=255)
                                                                                        ctgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaa                                                        <  7:4926/149‑1 (MQ=255)
                                                                                         tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                       >  3:112847/1‑149 (MQ=255)
                                                                                         tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                       >  6:52889/1‑149 (MQ=255)
                                                                                         tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                       >  3:127375/1‑149 (MQ=255)
                                                                                           cAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgc                                                                 >  5:210366/1‑137 (MQ=255)
                                                                                           cAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgc                                                                 <  6:210366/137‑1 (MQ=255)
                                                                                             aaCGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtgg                                                   >  3:332005/1‑149 (MQ=255)
                                                                                               cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtggt                                                  <  2:282289/148‑1 (MQ=255)
                                                                                               cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtggt                                                  >  1:282289/1‑148 (MQ=255)
                                                                                               cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGgtggtg                                                 >  4:8242/1‑149 (MQ=255)
                                                                                                   cGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTAGTTCGCCGCTGAAATCGTGCCGGTCATCGCACGCAAGAAGGTGGTGGAc                                              >  8:195536/1‑148 (MQ=255)
                                                                                                    gAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGAc                                              >  2:329871/1‑147 (MQ=255)
                                                                                                           tGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACa                                     <  7:191981/149‑1 (MQ=255)
                                                                                                             gCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAgg                                   >  7:76988/1‑149 (MQ=255)
                                                                                                             gCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                    <  2:154442/148‑1 (MQ=255)
                                                                                                               ccGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGCTCGCACGCAAGAAGGTGGTGGACAAGCTGACAGgtg                                 >  5:321476/1‑149 (MQ=255)
                                                                                                                 ggCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGGGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCGGGTGATCGCACGCAAGAAGGTGGTTGACAAGCTGACAGGTgtc                               <  4:112847/149‑1 (MQ=255)
                                                                                                                       aTGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAg                          <  4:127375/148‑1 (MQ=255)
                                                                                                                           cATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATg                     <  4:30924/149‑1 (MQ=255)
                                                                                                                               aGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCGGGTGATCGCACGCAAGAAGGTGGTTGACAAGCTGACAGGTGTCGTCAGCCATGAaga                 <  7:195536/149‑1 (MQ=255)
                                                                                                                                gCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAagag                >  2:103249/1‑149 (MQ=255)
                                                                                                                                 ccGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAgg               >  2:33035/1‑149 (MQ=255)
                                                                                                                                    cgAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgc            >  1:338304/1‑149 (MQ=255)
                                                                                                                                    cgAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgc            >  2:117900/1‑149 (MQ=255)
                                                                                                                                     gAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcg           >  1:143369/1‑149 (MQ=255)
                                                                                                                                       actgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcgc          <  3:101993/144‑1 (MQ=255)
                                                                                                                                       actgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcgc          >  4:101993/5‑148 (MQ=255)
                                                                                                                                       aGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGcc         <  6:255439/149‑1 (MQ=255)
                                                                                                                                         tgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                    >  5:325518/3‑120 (MQ=255)
                                                                                                                                         tgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                    <  6:325518/118‑1 (MQ=255)
                                                                                                                                          acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTa      >  6:281197/1‑149 (MQ=255)
                                                                                                                                          acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTa      <  3:8242/149‑1 (MQ=255)
                                                                                                                                          acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCGGAAATCGTGCCGGTGATCGCACGCAAGAAggtg                                                    <  8:266912/103‑1 (MQ=255)
                                                                                                                                          acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCGGAAATCGTGCCGGTGATCGCACGCAAGAAggtg                                                    >  7:266912/1‑103 (MQ=255)
                                                                                                                                           caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCt                                         <  1:330806/113‑1 (MQ=255)
                                                                                                                                           caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCt                                         >  2:330806/1‑113 (MQ=255)
                                                                                                                                           caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGt              >  6:376588/1‑140 (MQ=255)
                                                                                                                                           caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGt              <  5:376588/140‑1 (MQ=255)
                                                                                                                                            aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGt                                                                             >  8:181704/1‑76 (MQ=255)
                                                                                                                                            aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGt                                                                             <  7:181704/76‑1 (MQ=255)
                                                                                                                                            aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTAcc    <  2:143369/149‑1 (MQ=255)
                                                                                                                                             ggACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTAccc   <  5:353575/149‑1 (MQ=255)
                                                                                                                                              gACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCa  <  6:244888/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CCTGGTGCAGCAGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCA  >  NC_002947/2333431‑2333721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑