Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,333,579 T→G 5.4% Y171D (TAC→GAC)  fadA → 3‑ketoacyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,333,5790TG 5.4% 236.8 / 3.6 74Y171D (TAC→GAC) fadA3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (38/32);  new base G (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GCAGCAGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCAGGACG  >  NC_002947/2333437‑2333726
                                                                                                                                              |                                                                                                                                                   
gcagcaGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGccc                                                                                                                                               >  4:20430/1‑149 (MQ=255)
  agcagGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTg                                                                                                                                             <  3:20430/149‑1 (MQ=255)
      ggTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGt                                                                                                                                         <  3:137973/149‑1 (MQ=255)
       gTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTc                                                                                                                                        <  3:253729/149‑1 (MQ=255)
             aaCCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCgcagca                                                                                                                                  <  5:182011/149‑1 (MQ=255)
             aaCCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCgcag                                                                                                                                    >  7:219559/1‑147 (MQ=255)
                cAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAgcg                                                                                                                               >  7:290834/1‑149 (MQ=255)
                             aGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                                  <  8:219559/149‑1 (MQ=255)
                              ggCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                                  >  8:4926/1‑148 (MQ=255)
                               gCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGgcggcggcg                                                                                                                  >  2:129123/1‑147 (MQ=255)
                                 ttATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGAGGCGGCGCAgg                                                                                                              >  2:247794/1‑149 (MQ=255)
                                     gACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAggcggc                                                                                                          >  5:244888/1‑149 (MQ=255)
                                       ccccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGcggcgg                                                                                                        >  4:27190/1‑149 (MQ=255)
                                       ccccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGcggcgg                                                                                                        <  1:211684/149‑1 (MQ=255)
                                        cccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCggg                                                                                                       <  5:89833/149‑1 (MQ=255)
                                        cccGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCggg                                                                                                       >  3:30924/1‑149 (MQ=255)
                                          cgcgGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgt                                                                                                     >  4:174462/1‑149 (MQ=255)
                                               tATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTc                                                                                                <  1:129123/149‑1 (MQ=255)
                                                                  tGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCgg                                                                             <  8:290834/149‑1 (MQ=255)
                                                                   gCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgtt                                                                                                    <  7:256316/125‑1 (MQ=255)
                                                                   gCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGgtt                                                                                                    >  8:256316/1‑125 (MQ=255)
                                                                    ccTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGt                                                                            <  7:22517/148‑1 (MQ=255)
                                                                     cTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGa                                                                          >  5:170377/1‑149 (MQ=255)
                                                                        cATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcg                                                                       >  5:255439/1‑149 (MQ=255)
                                                                        cATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATc                                                                        <  3:27190/148‑1 (MQ=255)
                                                                          tGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgca                                                                     <  7:268601/149‑1 (MQ=255)
                                                                             cGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacg                                                                   >  1:154442/1‑148 (MQ=255)
                                                                             cGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacg                                                                   >  8:191981/1‑148 (MQ=255)
                                                                              gATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgcacgc                                                                  >  6:353575/1‑148 (MQ=255)
                                                                                  ctgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaa                                                             <  3:174462/149‑1 (MQ=255)
                                                                                  ctgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaa                                                             <  7:4926/149‑1 (MQ=255)
                                                                                   tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                            >  6:52889/1‑149 (MQ=255)
                                                                                   tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                            >  3:112847/1‑149 (MQ=255)
                                                                                   tgcAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCaagaag                                                            >  3:127375/1‑149 (MQ=255)
                                                                                     cAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgc                                                                      >  5:210366/1‑137 (MQ=255)
                                                                                     cAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATcgc                                                                      <  6:210366/137‑1 (MQ=255)
                                                                                       aaCGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtgg                                                        >  3:332005/1‑149 (MQ=255)
                                                                                         cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtggt                                                       <  2:282289/148‑1 (MQ=255)
                                                                                         cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAggtggt                                                       >  1:282289/1‑148 (MQ=255)
                                                                                         cGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGgtggtg                                                      >  4:8242/1‑149 (MQ=255)
                                                                                             cGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTAGTTCGCCGCTGAAATCGTGCCGGTCATCGCACGCAAGAAGGTGGTGGAc                                                   >  8:195536/1‑148 (MQ=255)
                                                                                              gAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGAc                                                   >  2:329871/1‑147 (MQ=255)
                                                                                                     tGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACa                                          <  7:191981/149‑1 (MQ=255)
                                                                                                       gCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAgg                                        >  7:76988/1‑149 (MQ=255)
                                                                                                       gCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                         <  2:154442/148‑1 (MQ=255)
                                                                                                         ccGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGCTCGCACGCAAGAAGGTGGTGGACAAGCTGACAGgtg                                      >  5:321476/1‑149 (MQ=255)
                                                                                                           ggCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGGGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCGGGTGATCGCACGCAAGAAGGTGGTTGACAAGCTGACAGGTgtc                                    <  4:112847/149‑1 (MQ=255)
                                                                                                                 aTGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAg                               <  4:127375/148‑1 (MQ=255)
                                                                                                                     cATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATg                          <  4:30924/149‑1 (MQ=255)
                                                                                                                         aGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCGGGTGATCGCACGCAAGAAGGTGGTTGACAAGCTGACAGGTGTCGTCAGCCATGAaga                      <  7:195536/149‑1 (MQ=255)
                                                                                                                          gCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAagag                     >  2:103249/1‑149 (MQ=255)
                                                                                                                           ccGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAgg                    >  2:33035/1‑149 (MQ=255)
                                                                                                                              cgAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgc                 >  1:338304/1‑149 (MQ=255)
                                                                                                                              cgAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgc                 >  2:117900/1‑149 (MQ=255)
                                                                                                                               gAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcg                >  1:143369/1‑149 (MQ=255)
                                                                                                                                 actgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcgc               >  4:101993/5‑148 (MQ=255)
                                                                                                                                 actgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTgcgc               <  3:101993/144‑1 (MQ=255)
                                                                                                                                 aGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGcc              <  6:255439/149‑1 (MQ=255)
                                                                                                                                   tgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                         <  6:325518/118‑1 (MQ=255)
                                                                                                                                   tgcaGGGCGTAGACGTCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAg                                         >  5:325518/3‑120 (MQ=255)
                                                                                                                                    acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTa           <  3:8242/149‑1 (MQ=255)
                                                                                                                                    acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTa           >  6:281197/1‑149 (MQ=255)
                                                                                                                                    acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCGGAAATCGTGCCGGTGATCGCACGCAAGAAggtg                                                         >  7:266912/1‑103 (MQ=255)
                                                                                                                                    acaGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCGGAAATCGTGCCGGTGATCGCACGCAAGAAggtg                                                         <  8:266912/103‑1 (MQ=255)
                                                                                                                                     caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCt                                              <  1:330806/113‑1 (MQ=255)
                                                                                                                                     caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCt                                              >  2:330806/1‑113 (MQ=255)
                                                                                                                                     caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGt                   >  6:376588/1‑140 (MQ=255)
                                                                                                                                     caGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGt                   <  5:376588/140‑1 (MQ=255)
                                                                                                                                      aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGt                                                                                  <  7:181704/76‑1 (MQ=255)
                                                                                                                                      aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGt                                                                                  >  8:181704/1‑76 (MQ=255)
                                                                                                                                      aGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTAcc         <  2:143369/149‑1 (MQ=255)
                                                                                                                                       ggACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTAccc        <  5:353575/149‑1 (MQ=255)
                                                                                                                                        gACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCa       <  6:244888/149‑1 (MQ=255)
                                                                                                                                            tCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCAGGAc   >  7:171512/1‑149 (MQ=255)
                                                                                                                                              tACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCAGGACg  >  4:180088/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GCAGCAGGTGCACAACCAGCTGGCCAGTGAGGCTTATGACCCCGCGGTATTGCGCATGAGCGAGCATGCCTACATGCCGATGCTGCAAACGGCCGAACGGGTGGCCCGGCGTTATGGCATAAGCCGCGAAGCACAGGACGTCTACGCCCTGCAGTCGCAGCAGCGCACGGCGGCGGCGCAGGCGGCGGGGTTGTTCGCCGCTGAAATCGTGCCGGTGATCGCACGCAAGAAGGTGGTGGACAAGCTGACAGGTGTCGTCAGCCATGAAGAGGTGCGCCTTACCCAGGACG  >  NC_002947/2333437‑2333726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑