Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,703 A→C 6.5% intergenic (+37/‑78) dctA‑II → / → yraO C4‑dicarboxylate transport protein/transporter YraO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,7030AC 6.5% 167.2 / 2.5 58intergenic (+37/‑78)dctA‑II/yraOC4‑dicarboxylate transport protein/transporter YraO
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base C (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AAAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGT  >  NC_002947/2340557‑2340847
                                                                                                                                                  |                                                                                                                                                
aaaaCGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                >  1:131426/1‑149 (MQ=255)
aaaaCGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                >  6:328890/1‑149 (MQ=255)
       cTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACGCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                <  1:209321/142‑1 (MQ=255)
       cTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                >  2:209321/1‑142 (MQ=255)
            aCCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGt                                                                                                                                     <  2:131426/148‑1 (MQ=255)
             ccGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTgg                                                                                                                                   >  7:63065/1‑149 (MQ=255)
              cGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTggg                                                                                                                                  <  5:328890/149‑1 (MQ=255)
               gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTgggg                                                                                                                                 >  1:332557/1‑149 (MQ=255)
                            caGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGt                                                                                                                    <  2:94315/149‑1 (MQ=255)
                                 ggCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgcg                                                                                                               >  8:131897/1‑149 (MQ=255)
                                 ggCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgcg                                                                                                               <  8:63065/149‑1 (MQ=255)
                                  gCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgcgc                                                                                                              >  6:39600/1‑149 (MQ=255)
                                           cGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTgg                                                                                                     <  8:182741/149‑1 (MQ=255)
                                                 ggtggtCACCCCCGTCGCGGAAACAGCCGACGGGGTCGCCGAACCACACCGCCAGGGCTGAGCCCCGCCACCCTCAAGCTTTTTGCAGGCCCCCACCCGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                               <  6:204621/149‑1 (MQ=255)
                                                     gtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGa                                                                                            <  3:346228/148‑1 (MQ=255)
                                                       cACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                         <  7:131897/149‑1 (MQ=255)
                                                        aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCCCCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGCGGCCACATAGACaa                                                                                         <  7:315779/148‑1 (MQ=255)
                                                        aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                         >  8:28880/1‑148 (MQ=255)
                                                        aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                         >  4:23163/1‑148 (MQ=255)
                                                         cccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa                                                                                       <  8:80053/149‑1 (MQ=255)
                                                            ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaa                                                                                     >  6:129263/1‑148 (MQ=255)
                                                             cGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaaa                                                                                   <  3:130404/149‑1 (MQ=255)
                                                              gTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAAc                                                                                  <  4:69190/149‑1 (MQ=255)
                                                                  cTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                              <  3:351772/149‑1 (MQ=255)
                                                                    gAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAagag                                                                             >  2:344644/1‑148 (MQ=255)
                                                                      aaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAc                                                                          <  2:332557/149‑1 (MQ=255)
                                                                                  ggTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTc                                                               <  2:156966/148‑1 (MQ=255)
                                                                                   gTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCg                                                              >  8:207655/1‑148 (MQ=255)
                                                                                   gTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCg                                                              >  4:337846/1‑148 (MQ=255)
                                                                                          aaCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCCCCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGACCAACCCATGCTCGCACTTCTg                                                      >  8:158845/1‑149 (MQ=255)
                                                                                          aaCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTg                                                      >  1:276049/1‑149 (MQ=255)
                                                                                           aCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTgg                                                     <  5:39600/149‑1 (MQ=255)
                                                                                            ccACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTggg                                                    >  8:126048/1‑149 (MQ=255)
                                                                                                acCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCt                                                 >  3:333512/1‑148 (MQ=255)
                                                                                                 ccGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGa                                               <  3:23163/149‑1 (MQ=255)
                                                                                                     cACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAt                                           >  3:35385/1‑149 (MQ=255)
                                                                                                      aCGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtg                                          <  1:344644/149‑1 (MQ=255)
                                                                                                      aCGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtg                                          <  7:126048/149‑1 (MQ=255)
                                                                                                         gCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtggtg                                       <  7:346622/149‑1 (MQ=255)
                                                                                                         gCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtggtg                                       <  7:343456/149‑1 (MQ=255)
                                                                                                            gAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATggtggt                                     >  3:317402/1‑148 (MQ=255)
                                                                                                             aGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATggtggt                                     <  5:129263/147‑1 (MQ=255)
                                                                                                             aGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGa                                   >  7:218970/1‑149 (MQ=255)
                                                                                                                    acacCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCac                            <  2:276049/149‑1 (MQ=255)
                                                                                                                    acacCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCac                            <  3:337846/149‑1 (MQ=255)
                                                                                                                     cacCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacc                           <  8:65570/149‑1 (MQ=255)
                                                                                                                      acCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCGGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTAGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacc                           >  2:363989/1‑148 (MQ=255)
                                                                                                                          tCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACCTAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGGCCTTCagctacct                      >  4:290441/1‑149 (MQ=255)
                                                                                                                            aaGCTTTTTGCAGGCCCCCACCAGGTGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGa                                               <  1:152121/122‑1 (MQ=255)
                                                                                                                            aaGCTTTTTGCAGGCCCCCACCAGGTGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAAAAACCATGCTCGCACTTCTGGGCCTGa                                               >  2:152121/1‑122 (MQ=255)
                                                                                                                            aaGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGa                    <  7:28880/149‑1 (MQ=255)
                                                                                                                               cTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCa                 <  7:207655/149‑1 (MQ=255)
                                                                                                                                tttttGGAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCAt                >  8:29529/1‑149 (MQ=255)
                                                                                                                                           ccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGa              >  4:224592/1‑140 (MQ=255)
                                                                                                                                           ccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGa              <  3:224592/140‑1 (MQ=255)
                                                                                                                                           ccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGGGACCTTCACCTACCTGATCATGAGTAACCGcc     >  2:153866/1‑149 (MQ=255)
                                                                                                                                           ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc     >  5:332931/1‑149 (MQ=255)
                                                                                                                                            cccACCAGGCGGTGCCGGGGGGGGGGCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt    <  4:35385/149‑1 (MQ=255)
                                                                                                                                              caccaGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGt  >  7:284653/1‑149 (MQ=255)
                                                                                                                                               accaGGCGTGGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt    <  3:268268/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AAAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGT  >  NC_002947/2340557‑2340847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑