Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,368,287 G→T 8.1% E369* (GAA→TAA) ‡ gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,368,2870GT 8.1% 184.1 / 6.6 62E369* (GAA→TAA) ‡gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/32);  new base T (3/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

CGGCAAGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368139‑2368432
                                                                                                                                                    |                                                                                                                                                    
cGGCAAGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTTGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAgg                                                                                                                                                      <  2:312120/149‑1 (MQ=255)
    aaGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACt                                                                                                                                                  >  5:283244/1‑149 (MQ=255)
     atgTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAATCGTGCATTCCATTCCGTACATCCGCGTGAAGGTTGACGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACtt                                                                                                                                                 <  8:54216/147‑2 (MQ=255)
     aGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTg                                                                                                                                                 <  2:316153/149‑1 (MQ=255)
      ggTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTgg                                                                                                                                                <  2:58780/149‑1 (MQ=255)
        tcatcaAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCCTTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGcc                                                                                                                                              >  6:269263/1‑149 (MQ=255)
             ccgatctaCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                          <  7:52383/141‑1 (MQ=255)
             aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATTCCATTCCGTACATCCGCGTGAAGGTGGCCGATGTCGAGCGCCGTTCGGGATTTGATCCCAAGGCGCACCTGGGCCAGTTCCTTGCCCAGGt                                                                                                                                         <  1:262661/149‑1 (MQ=255)
             aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAgg                                                                                                                                          <  1:166126/148‑1 (MQ=255)
             aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                         >  8:18122/1‑149 (MQ=255)
             aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                         >  8:210360/1‑149 (MQ=255)
               ggAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                        <  5:182490/148‑1 (MQ=255)
               ggAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                        >  6:182490/1‑148 (MQ=255)
                 aGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTg                                                                                                                                     <  7:40285/149‑1 (MQ=255)
                    aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                               <  6:10531/149‑1 (MQ=255)
                     ccGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                          >  8:52383/1‑140 (MQ=255)
                     ccGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑Ag                                                                                                                              >  4:62392/1‑149 (MQ=255)
                            aTGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGtgctgc                                                                                                                        <  7:11963/148‑1 (MQ=255)
                               gggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCcgcg                                                                                                                    >  3:134246/1‑149 (MQ=255)
                                  cTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcga                                                                                                                 >  6:9259/1‑149 (MQ=255)
                                    gTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                >  8:177967/1‑148 (MQ=255)
                                    gTACACCTCGTCGGTGTATGGCGAAAGCCTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGATCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgacg                                                                                                               >  5:224607/1‑149 (MQ=255)
                                       cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                             <  4:238960/148‑1 (MQ=255)
                                       cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                             <  7:210360/148‑1 (MQ=255)
                                       cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                             <  7:177967/148‑1 (MQ=255)
                                        acCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGccc                                                                                                                                             >  4:298675/1‑118 (MQ=255)
                                        acCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGccc                                                                                                                                             <  3:298675/118‑1 (MQ=255)
                                             gtcgGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                      <  3:25320/149‑1 (MQ=255)
                                              gaggTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCa                                                                                                     <  6:179077/147‑1 (MQ=255)
                                                      tGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGa                                                                                             <  4:99120/149‑1 (MQ=255)
                                                       ggCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGAt                                                                                            >  4:115477/1‑149 (MQ=255)
                                                         cGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                      <  7:305547/137‑1 (MQ=255)
                                                         cGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                      >  8:305547/1‑137 (MQ=255)
                                                          gAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGACGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATcga                                                                                         >  4:46580/1‑149 (MQ=255)
                                                           aaaGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATcgac                                                                                        <  4:134246/149‑1 (MQ=255)
                                                              gCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAg                                                                                     <  4:321941/149‑1 (MQ=255)
                                                                     tGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              <  6:127055/149‑1 (MQ=255)
                                                                               tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCAt                                                                    >  5:55816/1‑149 (MQ=255)
                                                                               tACATCCGCGTGAAGGTGGCCGAAGACGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCAt                                                                    <  3:46580/149‑1 (MQ=255)
                                                                                 cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATgg                                                                  <  5:269263/149‑1 (MQ=255)
                                                                                 cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATg                                                                   <  5:279616/148‑1 (MQ=255)
                                                                                          gAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGa                                                                                                   <  8:117098/107‑1 (MQ=255)
                                                                                          gAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGa                                                                                                   >  7:117098/1‑107 (MQ=255)
                                                                                           aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGGCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                         <  6:283244/148‑1 (MQ=255)
                                                                                            aGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCGAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTg                                                          >  3:325884/1‑146 (MQ=255)
                                                                                                ggCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                               <  2:363470/73‑1 (MQ=255)
                                                                                                ggCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                               >  1:363470/1‑73 (MQ=255)
                                                                                                 gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATc                                                    >  7:17746/1‑147 (MQ=255)
                                                                                                  ccGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 <  6:143293/149‑1 (MQ=255)
                                                                                                   cGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGATGTGGTGGTGCCCCCCGACGACCTTTTACACACCCCCAAGCACCACATGTTAAGCACGGGAATGGCAAACGTGCCAATcc                                                   >  1:115522/1‑149 (MQ=255)
                                                                                                   cGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 >  7:292468/1‑148 (MQ=255)
                                                                                                    gAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                               <  8:17746/149‑1 (MQ=255)
                                                                                                      aGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgc                                             <  6:55816/149‑1 (MQ=255)
                                                                                                            gcgcCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaaca                                       <  3:115477/149‑1 (MQ=255)
                                                                                                                     gggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATcc                                                   <  4:336700/128‑1 (MQ=255)
                                                                                                                     gggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATcc                                                   >  3:336700/1‑128 (MQ=255)
                                                                                                                      ggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCgt                             <  6:224607/149‑1 (MQ=255)
                                                                                                                         tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                >  7:75507/1‑63 (MQ=255)
                                                                                                                         tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                <  8:75507/63‑1 (MQ=255)
                                                                                                                                      agcACCTGGGCCAGTTCCTTGCCCAGGTGCTGGA‑‑‑AGTGCTGCCTCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGAc             >  7:130066/3‑149 (MQ=255)
                                                                                                                                         aCCTGGGCCAGTTCCTTGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCg          >  6:61093/1‑149 (MQ=255)
                                                                                                                                         aCCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGAAGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACACg          >  2:180762/1‑149 (MQ=255)
                                                                                                                                                 aaGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCg  >  2:215394/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGCAAGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368139‑2368432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑