Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,368,292 G→T 8.4% L370L (CTG→CTT)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,368,2920GT 8.4% 185.3 / 5.3 59L370L (CTG→CTT) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base T (3/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCGCTTCT  >  NC_002947/2368144‑2368437
                                                                                                                                                    |                                                                                                                                                    
aGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTg                                                                                                                                                      <  2:316153/149‑1 (MQ=255)
 ggTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTgg                                                                                                                                                     <  2:58780/149‑1 (MQ=255)
   tcatcaAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCCTTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGcc                                                                                                                                                   >  6:269263/1‑149 (MQ=255)
        ccgatctaCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                               <  7:52383/141‑1 (MQ=255)
        aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATTCCATTCCGTACATCCGCGTGAAGGTGGCCGATGTCGAGCGCCGTTCGGGATTTGATCCCAAGGCGCACCTGGGCCAGTTCCTTGCCCAGGt                                                                                                                                              <  1:262661/149‑1 (MQ=255)
        aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAgg                                                                                                                                               <  1:166126/148‑1 (MQ=255)
        aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                              >  8:210360/1‑149 (MQ=255)
        aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                              >  8:18122/1‑149 (MQ=255)
          ggAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                             >  6:182490/1‑148 (MQ=255)
          ggAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                             <  5:182490/148‑1 (MQ=255)
            aGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTg                                                                                                                                          <  7:40285/149‑1 (MQ=255)
               aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                                    <  6:10531/149‑1 (MQ=255)
                ccGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                               >  8:52383/1‑140 (MQ=255)
                ccGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑Ag                                                                                                                                   >  4:62392/1‑149 (MQ=255)
                       aTGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGtgctgc                                                                                                                             <  7:11963/148‑1 (MQ=255)
                          gggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCcgcg                                                                                                                         >  3:134246/1‑149 (MQ=255)
                             cTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcga                                                                                                                      >  6:9259/1‑149 (MQ=255)
                               gTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                     >  8:177967/1‑148 (MQ=255)
                               gTACACCTCGTCGGTGTATGGCGAAAGCCTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGATCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgacg                                                                                                                    >  5:224607/1‑149 (MQ=255)
                                  cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                                  <  4:238960/148‑1 (MQ=255)
                                  cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                                  <  7:177967/148‑1 (MQ=255)
                                  cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCgacgac                                                                                                                  <  7:210360/148‑1 (MQ=255)
                                   acCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGccc                                                                                                                                                  >  4:298675/1‑118 (MQ=255)
                                   acCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGccc                                                                                                                                                  <  3:298675/118‑1 (MQ=255)
                                        gtcgGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                           <  3:25320/149‑1 (MQ=255)
                                         gaggTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCa                                                                                                          <  6:179077/147‑1 (MQ=255)
                                                 tGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGa                                                                                                  <  4:99120/149‑1 (MQ=255)
                                                  ggCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGAt                                                                                                 >  4:115477/1‑149 (MQ=255)
                                                    cGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                           >  8:305547/1‑137 (MQ=255)
                                                    cGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTcc                                                                                                           <  7:305547/137‑1 (MQ=255)
                                                     gAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGACGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATcga                                                                                              >  4:46580/1‑149 (MQ=255)
                                                      aaaGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATcgac                                                                                             <  4:134246/149‑1 (MQ=255)
                                                         gCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAg                                                                                          <  4:321941/149‑1 (MQ=255)
                                                                tGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                                   <  6:127055/149‑1 (MQ=255)
                                                                          tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCAt                                                                         >  5:55816/1‑149 (MQ=255)
                                                                          tACATCCGCGTGAAGGTGGCCGAAGACGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCAt                                                                         <  3:46580/149‑1 (MQ=255)
                                                                            cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATgg                                                                       <  5:269263/149‑1 (MQ=255)
                                                                            cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATg                                                                        <  5:279616/148‑1 (MQ=255)
                                                                                     gAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGa                                                                                                        >  7:117098/1‑107 (MQ=255)
                                                                                     gAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGa                                                                                                        <  8:117098/107‑1 (MQ=255)
                                                                                      aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGGCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                              <  6:283244/148‑1 (MQ=255)
                                                                                       aGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCGAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTg                                                               >  3:325884/1‑146 (MQ=255)
                                                                                           ggCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                                    >  1:363470/1‑73 (MQ=255)
                                                                                           ggCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGa‑‑‑a                                                                                                                                    <  2:363470/73‑1 (MQ=255)
                                                                                            gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATc                                                         >  7:17746/1‑147 (MQ=255)
                                                                                             ccGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                      <  6:143293/149‑1 (MQ=255)
                                                                                              cGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGATGTGGTGGTGCCCCCCGACGACCTTTTACACACCCCCAAGCACCACATGTTAAGCACGGGAATGGCAAACGTGCCAATcc                                                        >  1:115522/1‑149 (MQ=255)
                                                                                              cGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                      >  7:292468/1‑148 (MQ=255)
                                                                                               gAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                                    <  8:17746/149‑1 (MQ=255)
                                                                                                 aGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgc                                                  <  6:55816/149‑1 (MQ=255)
                                                                                                       gcgcCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaaca                                            <  3:115477/149‑1 (MQ=255)
                                                                                                                gggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATcc                                                        <  4:336700/128‑1 (MQ=255)
                                                                                                                gggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATcc                                                        >  3:336700/1‑128 (MQ=255)
                                                                                                                 ggCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCgt                                  <  6:224607/149‑1 (MQ=255)
                                                                                                                    tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                     >  7:75507/1‑63 (MQ=255)
                                                                                                                    tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGcgac                                                                                                                     <  8:75507/63‑1 (MQ=255)
                                                                                                                                 agcACCTGGGCCAGTTCCTTGCCCAGGTGCTGGA‑‑‑AGTGCTGCCTCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGAc                  >  7:130066/3‑149 (MQ=255)
                                                                                                                                    aCCTGGGCCAGTTCCTTGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCg               >  6:61093/1‑149 (MQ=255)
                                                                                                                                    aCCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGAAGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACACg               >  2:180762/1‑149 (MQ=255)
                                                                                                                                            aaGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCg       >  2:215394/1‑149 (MQ=255)
                                                                                                                                                 aCTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCGCTTCt  >  7:343220/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGA‑‑‑AGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCGCTTCT  >  NC_002947/2368144‑2368437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑