Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,444,419 G→T 9.7% L157L (CTC→CTA) ‡ sulA ← cell division inhibitor SulA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,444,4190GT 9.7% 191.8 / 9.3 62L157L (CTC→CTA) ‡sulAcell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base T (3/3);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

CAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCTTT  >  NC_002947/2444272‑2444567
                                                                                                                                                   |                                                                                                                                                    
cAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGa                                                                                                                                                     <  8:320339/149‑1 (MQ=255)
           tCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGtt                                                                                                                                           >  6:194596/1‑148 (MQ=255)
             aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGATGCGGATGTTc                                                                                                                                          <  2:260470/147‑1 (MQ=255)
                  gggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCttt                                                                                                                                   <  6:254745/149‑1 (MQ=255)
                    gCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgt                                                                                                                                 <  3:57686/149‑1 (MQ=255)
                    gCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgt                                                                                                                                 <  5:194596/149‑1 (MQ=255)
                         cAGATGGCCGGTCGAGTGATGGATGGCGAGCTCGAGCGTGATGAAGGTCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGTCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCAGGCTTtgcctgt                                                                                                                             <  8:155312/148‑6 (MQ=37)
                                 cGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCAc                                                                                                                    >  2:331735/1‑149 (MQ=255)
                                  ggTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACt                                                                                                                   >  2:133894/1‑149 (MQ=255)
                                    tCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTgg                                                                                                                 >  4:344116/1‑149 (MQ=255)
                                           atggatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACg                                                                                                          >  2:156245/1‑149 (MQ=255)
                                             ggatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACg                                                                                                          >  3:213738/1‑147 (MQ=255)
                                              gatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCAtt                                                                                                                            <  8:239956/128‑1 (MQ=255)
                                              gatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCAtt                                                                                                                            >  7:239956/1‑128 (MQ=255)
                                                   cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgtt                                                                                                  <  3:344116/149‑1 (MQ=255)
                                                       cTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGttgttg                                                                                              <  8:344216/149‑1 (MQ=255)
                                                          gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGc                                                                                            <  1:156245/148‑1 (MQ=255)
                                                           aGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGt                                                                                          <  7:293523/149‑1 (MQ=255)
                                                                 atgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGc                                                                                     <  4:17328/148‑1 (MQ=255)
                                                                    aaGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCa                                                                                 <  4:150778/149‑1 (MQ=255)
                                                                    aaGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCa                                                                                 <  3:187559/149‑1 (MQ=255)
                                                                        gCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGa                                                                             >  4:22243/1‑149 (MQ=255)
                                                                            cgTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGtt                                                                         <  4:213738/149‑1 (MQ=255)
                                                                                   tgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                                  >  3:315452/1‑149 (MQ=255)
                                                                                          aGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTc                                                            <  1:331735/148‑1 (MQ=255)
                                                                                            tgtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTcac                                                          >  5:362747/1‑148 (MQ=255)
                                                                                                cagcagCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                                     <  4:315452/149‑1 (MQ=255)
                                                                                                  gcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGc                                                                                                             <  4:26801/91‑1 (MQ=255)
                                                                                                          tCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAg                                           >  7:350280/1‑149 (MQ=255)
                                                                                                             ggCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                                    >  4:16405/1‑137 (MQ=255)
                                                                                                             ggCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                                    <  3:16405/137‑1 (MQ=255)
                                                                                                             ggCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGAAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                                        >  1:303388/1‑149 (MQ=255)
                                                                                                              gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                                        >  4:255421/1‑148 (MQ=255)
                                                                                                                 aTTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAgg                                    >  2:351809/1‑149 (MQ=255)
                                                                                                                   ttGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTg                                  >  1:250153/1‑149 (MQ=255)
                                                                                                                      aaGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCa                                >  6:131079/1‑148 (MQ=255)
                                                                                                                           aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCt                          >  7:230171/1‑149 (MQ=255)
                                                                                                                               gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca                        >  5:43934/1‑147 (MQ=255)
                                                                                                                               gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca                        >  6:156377/1‑147 (MQ=255)
                                                                                                                                  aaGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                              <  7:76217/138‑1 (MQ=255)
                                                                                                                                  aaGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                              >  8:76217/1‑138 (MQ=255)
                                                                                                                                   aGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGcc                  <  8:350280/149‑1 (MQ=255)
                                                                                                                                    gCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                                     >  6:180896/1‑113 (MQ=255)
                                                                                                                                    gCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                                     <  5:180896/113‑1 (MQ=255)
                                                                                                                                     ccTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAg                <  5:156377/149‑1 (MQ=255)
                                                                                                                                      cTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCg                                                                                    >  1:345375/1‑80 (MQ=25)
                                                                                                                                      cTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCg                                                                                    <  2:345375/80‑1 (MQ=25)
                                                                                                                                      cTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt               >  2:171142/1‑149 (MQ=255)
                                                                                                                                      cTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt               >  8:92534/1‑149 (MQ=255)
                                                                                                                                      cTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt               >  5:358792/1‑149 (MQ=255)
                                                                                                                                      cTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt               >  1:24080/1‑149 (MQ=255)
                                                                                                                                       tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcaca                      <  2:286890/141‑1 (MQ=255)
                                                                                                                                       tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcaca                      >  1:286890/1‑141 (MQ=255)
                                                                                                                                       tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt              >  4:210995/1‑149 (MQ=255)
                                                                                                                                        gAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAtt                                                                               <  7:259798/83‑1 (MQ=255)
                                                                                                                                        gAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAtt                                                                               >  8:259798/1‑83 (MQ=255)
                                                                                                                                        gAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTg             >  8:102776/1‑149 (MQ=255)
                                                                                                                                          aCATCAGGCGATGCGTATGTTCAGGCTTTGTGCATTTCGGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCa           <  7:105905/149‑1 (MQ=255)
                                                                                                                                          aCATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCa           <  3:22243/149‑1 (MQ=255)
                                                                                                                                             tCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcg        >  8:272978/1‑149 (MQ=255)
                                                                                                                                                gCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                     <  7:151513/117‑1 (MQ=255)
                                                                                                                                                gCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                     >  8:151513/1‑117 (MQ=255)
                                                                                                                                                   gAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCttt  <  5:131079/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCTTT  >  NC_002947/2444272‑2444567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑