Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,461,359 G→T 6.7% A120S (GCT→TCT) ‡ lolC → lipoprotein releasing system transmembrane protein LolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,461,3590GT 6.7% 186.3 / 4.1 60A120S (GCT→TCT) ‡lolClipoprotein releasing system transmembrane protein LolC
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base T (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

CCAGCCCATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGC  >  NC_002947/2461214‑2461507
                                                                                                                                                 |                                                                                                                                                    
ccAGCCCATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTc                                                                                                                                                   <  6:279746/149‑1 (MQ=255)
    cccATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcga                                                                                                                                               <  6:316802/149‑1 (MQ=255)
      cATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagc                                                                                                                                             >  4:153598/1‑149 (MQ=255)
        ttGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgc                                                                                                                                           >  4:361640/1‑149 (MQ=255)
           ccGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgcga                                                                                                                                         <  7:10660/148‑1 (MQ=255)
            cGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCAGCTCGCGAGCGCGAgg                                                                                                                                       >  6:226132/1‑149 (MQ=255)
                  gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                 >  6:278540/1‑149 (MQ=255)
                  gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                 <  1:60880/149‑1 (MQ=255)
                   cAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                 >  5:60140/1‑148 (MQ=255)
                    aGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGat                                                                                                                                >  7:142767/1‑148 (MQ=255)
                      gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCTTTCACCCAGATGCAGGGCCTGCTGACCCATGATGTCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                             <  8:252166/149‑1 (MQ=255)
                      gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                             >  6:67900/1‑149 (MQ=255)
                            gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc                                                                                                                         <  3:153598/147‑1 (MQ=255)
                            gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc                                                                                                                         <  3:281290/147‑1 (MQ=255)
                                aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc                                                                                                                   >  4:176871/1‑149 (MQ=255)
                                aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc                                                                                                                   <  8:142767/149‑1 (MQ=255)
                                   aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGTTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                <  5:226132/149‑1 (MQ=255)
                                   aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                <  3:272508/149‑1 (MQ=255)
                                   aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                <  5:359163/149‑1 (MQ=255)
                                     gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                             >  7:187118/1‑134 (MQ=255)
                                     gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTccc                                                                                                              >  2:136552/1‑148 (MQ=255)
                                             gAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGgcc                                                                                                      >  3:297018/1‑149 (MQ=255)
                                                            aGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTgg                                                                                       >  4:180136/1‑149 (MQ=255)
                                                                gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc                                                                                                                               <  7:351474/105‑1 (MQ=255)
                                                                gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc                                                                                                                               >  8:351474/1‑105 (MQ=255)
                                                                    cgcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                               <  3:180136/149‑1 (MQ=255)
                                                                     gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                               >  5:254130/1‑148 (MQ=255)
                                                                     gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                               >  1:235194/1‑148 (MQ=255)
                                                                        gTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt                                                                           >  7:346570/1‑149 (MQ=255)
                                                                        gTTCACCCAGATGCAGGGCCTGCTGACCCATGAGGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt                                                                           <  7:343054/149‑1 (MQ=255)
                                                                         ttCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTg                                                                          >  7:20634/1‑149 (MQ=255)
                                                                            aCCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                        <  5:278540/148‑1 (MQ=255)
                                                                              ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca                                                                     >  7:180293/1‑149 (MQ=255)
                                                                              ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca                                                                     >  8:226347/1‑149 (MQ=255)
                                                                                              ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                        <  4:207059/130‑1 (MQ=255)
                                                                                              ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                        >  3:207059/1‑130 (MQ=255)
                                                                                                   ccATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcgg                                                >  8:7414/1‑149 (MQ=255)
                                                                                                    cATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAAGTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                               >  1:118640/1‑149 (MQ=255)
                                                                                                                 tGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGc                                  <  8:346570/149‑1 (MQ=255)
                                                                                                                  gCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCg                                 >  2:272588/1‑149 (MQ=255)
                                                                                                                    aGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCCGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGtt                               <  1:136552/149‑1 (MQ=255)
                                                                                                                      aaGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGc                             <  4:297018/149‑1 (MQ=255)
                                                                                                                         gTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                          <  5:67900/149‑1 (MQ=255)
                                                                                                                             tgctCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGc                      >  2:356442/1‑149 (MQ=255)
                                                                                                                                tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg                                                                                  >  3:312363/1‑86 (MQ=255)
                                                                                                                                tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg                                                                                  <  4:312363/86‑1 (MQ=255)
                                                                                                                                  aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc                   >  1:28667/1‑147 (MQ=255)
                                                                                                                                  aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc                   >  5:123381/1‑147 (MQ=255)
                                                                                                                                   aTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAg                <  8:20634/149‑1 (MQ=255)
                                                                                                                                     atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                          <  4:303110/134‑1 (MQ=38)
                                                                                                                                     atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                          >  3:303110/4‑137 (MQ=38)
                                                                                                                                      gCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCt              <  6:254130/148‑1 (MQ=255)
                                                                                                                                         tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                               <  4:146854/112‑1 (MQ=37)
                                                                                                                                         tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                               >  3:146854/1‑112 (MQ=37)
                                                                                                                                              cgcGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCTTCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtc       <  6:226745/145‑1 (MQ=255)
                                                                                                                                              cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgt     <  1:356442/149‑1 (MQ=255)
                                                                                                                                              cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCACTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcga     >  5:226745/1‑148 (MQ=255)
                                                                                                                                               ccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtc    <  2:308039/149‑1 (MQ=255)
                                                                                                                                                cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg   <  3:361640/149‑1 (MQ=255)
                                                                                                                                                cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg   >  3:175893/1‑149 (MQ=255)
                                                                                                                                                 gCTCGCGAGCGCGAGGTGTCGATGATCGACAACTTCGTCCTGCAAGGACGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGc  >  7:55773/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCAGCCCATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGC  >  NC_002947/2461214‑2461507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑