Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,461,366 A→C 7.2% E122A (GAG→GCG) ‡ lolC → lipoprotein releasing system transmembrane protein LolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,461,3660AC 7.2% 160.8 / 4.8 56E122A (GAG→GCG) ‡lolClipoprotein releasing system transmembrane protein LolC
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base C (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

CCCATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGCCCCC  >  NC_002947/2461218‑2461511
                                                                                                                                                    |                                                                                                                                                 
cccATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcga                                                                                                                                                   <  6:316802/149‑1 (MQ=255)
  cATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagc                                                                                                                                                 >  4:153598/1‑149 (MQ=255)
    ttGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgc                                                                                                                                               >  4:361640/1‑149 (MQ=255)
       ccGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgcga                                                                                                                                             <  7:10660/148‑1 (MQ=255)
        cGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCAGCTCGCGAGCGCGAgg                                                                                                                                           >  6:226132/1‑149 (MQ=255)
              gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                     >  6:278540/1‑149 (MQ=255)
              gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                     <  1:60880/149‑1 (MQ=255)
               cAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa                                                                                                                                     >  5:60140/1‑148 (MQ=255)
                aGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGat                                                                                                                                    >  7:142767/1‑148 (MQ=255)
                  gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCTTTCACCCAGATGCAGGGCCTGCTGACCCATGATGTCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                                 <  8:252166/149‑1 (MQ=255)
                  gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                                 >  6:67900/1‑149 (MQ=255)
                        gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc                                                                                                                             <  3:153598/147‑1 (MQ=255)
                        gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc                                                                                                                             <  3:281290/147‑1 (MQ=255)
                            aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc                                                                                                                       <  8:142767/149‑1 (MQ=255)
                            aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc                                                                                                                       >  4:176871/1‑149 (MQ=255)
                               aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGTTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                    <  5:226132/149‑1 (MQ=255)
                               aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                    <  5:359163/149‑1 (MQ=255)
                               aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc                                                                                                                    <  3:272508/149‑1 (MQ=255)
                                 gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat                                                                                                                                 >  7:187118/1‑134 (MQ=255)
                                 gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTccc                                                                                                                  >  2:136552/1‑148 (MQ=255)
                                         gAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGgcc                                                                                                          >  3:297018/1‑149 (MQ=255)
                                                        aGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTgg                                                                                           >  4:180136/1‑149 (MQ=255)
                                                            gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc                                                                                                                                   <  7:351474/105‑1 (MQ=255)
                                                            gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc                                                                                                                                   >  8:351474/1‑105 (MQ=255)
                                                                cgcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                                   <  3:180136/149‑1 (MQ=255)
                                                                 gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                                   >  5:254130/1‑148 (MQ=255)
                                                                 gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc                                                                                   >  1:235194/1‑148 (MQ=255)
                                                                    gTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt                                                                               >  7:346570/1‑149 (MQ=255)
                                                                    gTTCACCCAGATGCAGGGCCTGCTGACCCATGAGGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt                                                                               <  7:343054/149‑1 (MQ=255)
                                                                     ttCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTg                                                                              >  7:20634/1‑149 (MQ=255)
                                                                        aCCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                            <  5:278540/148‑1 (MQ=255)
                                                                          ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca                                                                         >  7:180293/1‑149 (MQ=255)
                                                                          ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca                                                                         >  8:226347/1‑149 (MQ=255)
                                                                                          ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                            <  4:207059/130‑1 (MQ=255)
                                                                                          ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg                                                                            >  3:207059/1‑130 (MQ=255)
                                                                                               ccATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcgg                                                    >  8:7414/1‑149 (MQ=255)
                                                                                                cATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAAGTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                                   >  1:118640/1‑149 (MQ=255)
                                                                                                             tGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGc                                      <  8:346570/149‑1 (MQ=255)
                                                                                                              gCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCg                                     >  2:272588/1‑149 (MQ=255)
                                                                                                                aGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCCGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGtt                                   <  1:136552/149‑1 (MQ=255)
                                                                                                                  aaGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGc                                 <  4:297018/149‑1 (MQ=255)
                                                                                                                     gTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                              <  5:67900/149‑1 (MQ=255)
                                                                                                                         tgctCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGc                          >  2:356442/1‑149 (MQ=255)
                                                                                                                            tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg                                                                                      <  4:312363/86‑1 (MQ=255)
                                                                                                                            tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg                                                                                      >  3:312363/1‑86 (MQ=255)
                                                                                                                              aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc                       >  1:28667/1‑147 (MQ=255)
                                                                                                                              aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc                       >  5:123381/1‑147 (MQ=255)
                                                                                                                               aTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAg                    <  8:20634/149‑1 (MQ=255)
                                                                                                                                 atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                              <  4:303110/134‑1 (MQ=38)
                                                                                                                                 atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt                              >  3:303110/4‑137 (MQ=38)
                                                                                                                                  gCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCt                  <  6:254130/148‑1 (MQ=255)
                                                                                                                                     tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                                   <  4:146854/112‑1 (MQ=37)
                                                                                                                                     tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc                                                   >  3:146854/1‑112 (MQ=37)
                                                                                                                                          cgcGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCTTCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtc           <  6:226745/145‑1 (MQ=255)
                                                                                                                                          cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgt         <  1:356442/149‑1 (MQ=255)
                                                                                                                                          cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCACTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcga         >  5:226745/1‑148 (MQ=255)
                                                                                                                                           ccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtc        <  2:308039/149‑1 (MQ=255)
                                                                                                                                            cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg       >  3:175893/1‑149 (MQ=255)
                                                                                                                                            cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg       <  3:361640/149‑1 (MQ=255)
                                                                                                                                             gCTCGCGAGCGCGAGGTGTCGATGATCGACAACTTCGTCCTGCAAGGACGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGc      >  7:55773/1‑149 (MQ=255)
                                                                                                                                                 gcgagtgcgagGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGccccc  >  7:159036/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCCATTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGCCCCC  >  NC_002947/2461218‑2461511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑