| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,461,370 | C→A | 7.1% | R123R (CGC→CGA) | lolC → | lipoprotein releasing system transmembrane protein LolC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,461,370 | 0 | C | A | 7.1% | 178.8 / 3.9 | 56 | R123R (CGC→CGA) | lolC | lipoprotein releasing system transmembrane protein LolC |
| Reads supporting (aligned to +/- strand): ref base C (28/24); new base A (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
TTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGCCCCC > NC_002947/2461222‑2461511 | ttGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgc > 4:361640/1‑149 (MQ=255) ccGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTcgcgagcgcga < 7:10660/148‑1 (MQ=255) cGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCAGCTCGCGAGCGCGAgg > 6:226132/1‑149 (MQ=255) gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa > 6:278540/1‑149 (MQ=255) gCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa < 1:60880/149‑1 (MQ=255) cAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGa > 5:60140/1‑148 (MQ=255) aGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGat > 7:142767/1‑148 (MQ=255) gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCTTTCACCCAGATGCAGGGCCTGCTGACCCATGATGTCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat < 8:252166/149‑1 (MQ=255) gCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat > 6:67900/1‑149 (MQ=255) gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc < 3:281290/147‑1 (MQ=255) gCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGAc < 3:153598/147‑1 (MQ=255) aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc < 8:142767/149‑1 (MQ=255) aacaaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTc > 4:176871/1‑149 (MQ=255) aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGTTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc < 5:226132/149‑1 (MQ=255) aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc < 5:359163/149‑1 (MQ=255) aaGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTc < 3:272508/149‑1 (MQ=255) gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatcat > 7:187118/1‑134 (MQ=255) gTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTccc > 2:136552/1‑148 (MQ=255) gAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGgcc > 3:297018/1‑149 (MQ=255) aGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTgg > 4:180136/1‑149 (MQ=255) gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc < 7:351474/105‑1 (MQ=255) gTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGatc > 8:351474/1‑105 (MQ=255) cgcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc < 3:180136/149‑1 (MQ=255) gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc > 5:254130/1‑148 (MQ=255) gcCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGc > 1:235194/1‑148 (MQ=255) gTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt > 7:346570/1‑149 (MQ=255) gTTCACCCAGATGCAGGGCCTGCTGACCCATGAGGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGt < 7:343054/149‑1 (MQ=255) ttCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTg > 7:20634/1‑149 (MQ=255) aCCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg < 5:278540/148‑1 (MQ=255) ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca > 7:180293/1‑149 (MQ=255) ccAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGca > 8:226347/1‑149 (MQ=255) ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg < 4:207059/130‑1 (MQ=255) ctgACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTggg > 3:207059/1‑130 (MQ=255) ccATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcgg > 8:7414/1‑149 (MQ=255) cATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAAGTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc > 1:118640/1‑149 (MQ=255) tGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGc < 8:346570/149‑1 (MQ=255) gCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCg > 2:272588/1‑149 (MQ=255) aGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCCGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGtt < 1:136552/149‑1 (MQ=255) aaGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGc < 4:297018/149‑1 (MQ=255) gTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt < 5:67900/149‑1 (MQ=255) tgctCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGc > 2:356442/1‑149 (MQ=255) tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg < 4:312363/86‑1 (MQ=255) tCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCg > 3:312363/1‑86 (MQ=255) aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc > 1:28667/1‑147 (MQ=255) aaTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGAc > 5:123381/1‑147 (MQ=255) aTGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAg < 8:20634/149‑1 (MQ=255) atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt < 4:303110/134‑1 (MQ=38) atgaTCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCAt > 3:303110/4‑137 (MQ=38) gCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCt < 6:254130/148‑1 (MQ=255) tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc < 4:146854/112‑1 (MQ=37) tCGACACCTCGCGCTCGCGAGCGGGGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAggcggc > 3:146854/1‑112 (MQ=37) cgcGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCTTCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtc < 6:226745/145‑1 (MQ=255) cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgt < 1:356442/149‑1 (MQ=255) cccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCACTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcga > 5:226745/1‑148 (MQ=255) ccGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtc < 2:308039/149‑1 (MQ=255) cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg < 3:361640/149‑1 (MQ=255) cGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTtcgtcg > 3:175893/1‑149 (MQ=255) gCTCGCGAGCGCGAGGTGTCGATGATCGACAACTTCGTCCTGCAAGGACGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGc > 7:55773/1‑149 (MQ=255) gcgagtgcgagGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGccccc > 7:159036/1‑149 (MQ=255) | TTGCCGACTGGCAGGCCCTTGCCCAACAAGTAAAGCAGAATCCGCAGGTGGTAGCGGTTGCGCCGTTCACCCAGATGCAGGGCCTGCTGACCCATGATGGCAAGGTGCAGAAGGTGCTGCTCAATGGCATCGACCCCGCTCGCGAGCGCGAGGTGTCGATCATCGACAACTTCGTCCTGCAAGGCCGCCTGGACCAGTTGGCGCCGGGCGAGTGGGGCATCATGATCGGCGACAAGGCGGCGGCCAAGCTCGGCGTTGCCATCGGCGACAAGCTCACCTTCGTCGCCCCC > NC_002947/2461222‑2461511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |