Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,483,400	T→A	9.2%	L89Q (CTG→CAG)	puuA‑I →	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,483,400	0	T	A	9.2%	174.1 / 7.2	65	L89Q (CTG→CAG)	puuA‑I	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base A (2/4);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GCTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTGGCAT  >  NC_002947/2483253‑2483547                                                                                                                                                    |                                                                                                                                                     gcTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTg                                                                                                                                                     <  6:65827/149‑1 (MQ=255) gcTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTg                                                                                                                                                     <  1:336565/149‑1 (MQ=255)  gctacccATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTgg                                                                                                                                                    <  2:312990/145‑1 (MQ=255)        cATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCgg                                                                                                                                              >  2:91615/1‑149 (MQ=255)        cATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCgg                                                                                                                                              <  7:5477/149‑1 (MQ=255)        cATGGACATGCTGGAAAAAGTCGCAGCCGGTACGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCgg                                                                                                                                              <  6:273076/149‑1 (MQ=255)          tGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCACCAGGCGCCgggc                                                                                                                                            <  5:81073/149‑2 (MQ=255)            gACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCAACGGCGACCCGGATGCTACCCGGCACCAGGCGCCgggca                                                                                                                                           <  1:80280/148‑3 (MQ=255)              cATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCTCTGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑c                                                                                                                                       <  6:214606/149‑1 (MQ=255)               aTGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTg                                                                                                                                                     >  1:244474/1‑135 (MQ=255)               aTGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTg                                                                                                                                                     <  2:244474/135‑1 (MQ=255)                  cTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACA‑CTg                                                                                                                                     >  7:43472/1‑147 (MQ=255)                   tGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAg                                                                                                                                  >  1:64048/1‑149 (MQ=255)                     gAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGcc                                                                                                                                >  7:296309/1‑149 (MQ=255)                       aaaaGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCCGGGCGGCCTTTCCCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCgg                                                                                                                              <  5:124078/149‑1 (MQ=255)                        aaaGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGTAGCCGGt                                                                                                                             >  7:250849/1‑149 (MQ=255)                            tCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTgcgc                                                                                                                         <  2:64048/149‑1 (MQ=255)                                         gcCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAg                                                                                                            >  6:84178/1‑149 (MQ=255)                                           ctctGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGt                                                                                                           >  6:275691/1‑148 (MQ=255)                                                aaGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATc                                                                                                       <  8:296309/147‑1 (MQ=255)                                                aaGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCg                                                                                                      <  4:228388/148‑1 (MQ=255)                                                aaGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGc                                                                                                     >  5:127566/1‑149 (MQ=255)                                                             aCTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGAt                                                                                         <  6:27410/148‑1 (MQ=255)                                                                      tgctgGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGAc                                                                               >  5:124987/1‑149 (MQ=255)                                                                           ggTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCAc                                                                          >  8:326325/1‑149 (MQ=255)                                                                            gTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGa                                                                                                         <  1:100317/117‑1 (MQ=255)                                                                            gTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGa                                                                                                         >  2:100317/1‑117 (MQ=255)                                                                            gTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACt                                                                         <  8:337874/149‑1 (MQ=255)                                                                               cccAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGc                                                                       <  6:127566/148‑1 (MQ=255)                                                                                ccAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCa                                                                      >  5:243649/1‑148 (MQ=255)                                                                                   gggCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTTATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGc                                                                  <  5:84178/149‑1 (MQ=255)                                                                                          cTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTg                                                                        <  3:136339/136‑1 (MQ=255)                                                                                          cTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTg                                                                        >  4:136339/1‑136 (MQ=255)                                                                                           tttACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCt                                                                                            <  7:80129/115‑1 (MQ=255)                                                                                           tttACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCt                                                                                            >  8:80129/1‑115 (MQ=255)                                                                                             tACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAAtt                                                        >  8:31079/1‑149 (MQ=255)                                                                                              aCCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTc                                                       >  5:54369/1‑149 (MQ=255)                                                                                               cccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGa                                                               >  1:263711/1‑140 (MQ=255)                                                                                               cccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGa                                                               <  2:263711/140‑1 (MQ=255)                                                                                                ccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTc                                                       >  4:101361/1‑147 (MQ=255)                                                                                                  gATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGc                                                   >  1:208248/1‑149 (MQ=255)                                                                                                       gTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGt                                              >  7:108859/1‑149 (MQ=255)                                                                                                          aCTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTg                                             >  1:245204/1‑147 (MQ=255)                                                                                                                 cTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTgg                                    >  6:265852/1‑149 (MQ=255)                                                                                                                      ccGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgt                               >  5:251152/1‑149 (MQ=255)                                                                                                                       cGACGGCGACCCGTATGCTGCCCGGCGCCTGGGGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtg                              <  8:250849/149‑1 (MQ=255)                                                                                                                       cGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtg                              <  1:148778/149‑1 (MQ=255)                                                                                                                        gACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtgt                             >  7:62375/1‑149 (MQ=255)                                                                                                                        gACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtgt                             >  7:57278/1‑149 (MQ=255)                                                                                                                         aCGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGtt                            <  6:251152/149‑1 (MQ=255)                                                                                                                            gCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGttctt                         <  2:245204/149‑1 (MQ=255)                                                                                                                             cGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCAGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGttctt                         >  2:99568/1‑148 (MQ=255)                                                                                                                              gACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCa                       <  6:124987/149‑1 (MQ=255)                                                                                                                                 ccGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCaaa                                                                    <  7:34575/101‑1 (MQ=255)                                                                                                                                 ccGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCaaa                                                                    >  8:34575/1‑101 (MQ=255)                                                                                                                                       gctgcCCGGCGCCTGGTGCCGGGTACGACTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTgg               >  1:16989/1‑149 (MQ=255)                                                                                                                                       gctgcCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGc              >  4:206734/1‑149 (MQ=255)                                                                                                                                        gtacccGGCACCAGGCGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtg                              <  2:128699/129‑1 (MQ=255)                                                                                                                                        gtacccGGCACCAGGCGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtg                              >  1:128699/4‑132 (MQ=255)                                                                                                                                        ctgcCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGc              <  6:243649/148‑1 (MQ=255)                                                                                                                                          acccGGCACCAGGCGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGc                                                                                             >  6:235250/2‑67 (MQ=38)                                                                                                                                          acccGGCACCAGGCGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGc                                                                                             <  5:235250/66‑1 (MQ=38)                                                                                                                                           cccGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAg          >  3:176589/1‑149 (MQ=255)                                                                                                                                               gcgcCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTg      <  1:99568/149‑1 (MQ=255)                                                                                                                                                   cTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTGGCAt  <  3:206734/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACG‑CTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTGGCAT  >  NC_002947/2483253‑2483547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑