Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,567,596 G→A 10.0% A111V (GCC→GTC) ‡ PP_2250 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,567,5960GA10.0% 136.2 / 4.9 50A111V (GCC→GTC) ‡PP_2250LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/26);  new base A (2/3);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

GGAAGCCGCGGCGGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCGTGTGGCTCCAG  >  NC_002947/2567447‑2567732
                                                                                                                                                     |                                                                                                                                        
ggAAGCCGCGGCGGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCA‑GGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATgg                                                                                                                                          >  8:66345/1‑149 (MQ=255)
 gAAGCCGCGGCGGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATgg                                                                                                                                          >  8:40327/1‑149 (MQ=255)
     ccGCGGCGGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGgccgc                                                                                                                                      >  8:270973/1‑149 (MQ=255)
       gcggcgGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGgccgc                                                                                                                                      >  5:307963/1‑147 (MQ=255)
          gcggGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCcagca                                                                                                                                 <  5:75665/149‑1 (MQ=255)
                 tCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAgcgggcg                                                                                                                          <  1:169384/149‑1 (MQ=255)
                    aCCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCA‑GGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGa                                                                                                                      <  7:66345/149‑1 (MQ=255)
                     ccTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGa                                                                                                                      >  6:304764/1‑149 (MQ=255)
                         cAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGATCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATc                                                                                                                    <  8:67586/147‑1 (MQ=255)
                            gCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATCACCGCCCGCTGCTGGCGGCCATCgccaa                                                                                                               <  5:19525/149‑5 (MQ=255)
                              cGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCg                                                                                                             >  6:22741/1‑149 (MQ=255)
                                gCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAgcg                                                                                                                              >  1:215235/1‑130 (MQ=255)
                                gCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAgcg                                                                                                                              <  2:215235/130‑1 (MQ=255)
                                 cAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGt                                                                                                          >  3:308682/1‑149 (MQ=255)
                                  aaaGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTg                                                                                                         >  5:105872/1‑149 (MQ=255)
                                    aGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATCACCGCCCGCTgctggcggccatc                                                                                                                    >  7:117324/1‑131 (MQ=255)
                                    aGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATCACCGCCCGCTgctggcggccatc                                                                                                                    >  7:340577/1‑131 (MQ=255)
                                    aGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATCACCGCCCGCTgctggcggccatc                                                                                                                    <  8:117324/136‑6 (MQ=255)
                                    aGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATCACCGCCCGCTgctggcggccatc                                                                                                                    <  8:340577/136‑6 (MQ=255)
                                     ggCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCa                                                                                                      >  4:85279/1‑149 (MQ=255)
                                        tACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTg                                                                                                   >  3:142334/1‑149 (MQ=255)
                                             agagGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAgg                                                                                              >  8:199382/1‑149 (MQ=255)
                                              gagGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAggg                                                                                             <  3:56914/149‑1 (MQ=255)
                                                  tCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAg                                                                                         >  2:346974/1‑149 (MQ=255)
                                                    ggCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAgg                                                                                        <  4:28482/148‑1 (MQ=255)
                                                         ggCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGctc                                                                                  >  6:130219/1‑149 (MQ=255)
                                                          gCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGctct                                                                                 <  1:132043/149‑1 (MQ=255)
                                                            cGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCtctc                                                                                >  7:49998/1‑148 (MQ=255)
                                                             gCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCa                                                                              <  5:86953/149‑1 (MQ=255)
                                                             gCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCa                                                                              <  7:270973/149‑1 (MQ=255)
                                                                    aGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTg                                                                       <  4:81651/149‑1 (MQ=255)
                                                                    aGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTg                                                                       <  4:76590/149‑1 (MQ=255)
                                                                     gCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCATGGTCAGGCTGCTCTCCAGCCCTTgg                                                                      <  4:308682/149‑1 (MQ=255)
                                                                      cAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTgg                                                                      >  3:243965/1‑148 (MQ=255)
                                                                            cAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCt                                                               <  1:243014/149‑1 (MQ=255)
                                                                             aGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTc                                                              <  7:40327/149‑1 (MQ=255)
                                                                             aGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTc                                                              >  4:236127/1‑149 (MQ=255)
                                                                                 cttcttCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCaga                                                          <  5:22741/149‑1 (MQ=255)
                                                                                 cttcttCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCaga                                                          <  6:105872/149‑1 (MQ=255)
                                                                                 cttcttCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCaga                                                          <  4:19542/149‑1 (MQ=255)
                                                                                 cttcttCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCa                                                            >  3:19542/1‑147 (MQ=255)
                                                                                       ccTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATg                                                     <  1:346974/148‑1 (MQ=255)
                                                                                        cTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGaa                                                   <  7:199382/149‑1 (MQ=255)
                                                                                                      gCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGccc                                      >  2:19355/1‑148 (MQ=255)
                                                                                                          ggaaaTATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGACAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCAGCAGCAGCCCCAgta                                 >  6:112883/4‑148 (MQ=255)
                                                                                                              atatCGAGCATTGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGGAGCGGGAGGTGATCGATGTCTGGTGCCACTGCCAGGGTCAGGCTGCTCTCCTGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTc                             <  5:112883/149‑1 (MQ=255)
                                                                                                                    aGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCaa                        >  5:212332/1‑148 (MQ=255)
                                                                                                                    aGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAAt                       >  5:141843/1‑149 (MQ=255)
                                                                                                                           ggTGGCGCTTACCTAGGTTGGCGATGGCCACCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTg                <  7:249181/149‑1 (MQ=255)
                                                                                                                                ctcTTACCTAGGTTGGCGATTGCCGACATCAGCGGGAGGTGATCGATGTCGGTTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTACAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCgtg           <  4:243965/147‑1 (MQ=255)
                                                                                                                                cgcTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCgtg           <  6:307963/149‑1 (MQ=255)
                                                                                                                                  cTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCGtgtg         <  1:19355/149‑1 (MQ=255)
                                                                                                                                     aCCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCGTGTgg        <  4:142334/147‑1 (MQ=255)
                                                                                                                                         aGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCGTGTGGCTCCAg  >  3:260449/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                        
GGAAGCCGCGGCGGGCATCGACCTGCAGGCCGGCAAAGGCTACGCAGAGGTCGGCACGGCCGCTGTCCAGCAAGTGCAGGGCTTCTTCCTGCGGGGCGCTGAGCAGGGCAATATCCAGCAATGGGTGGCGCTTACCTAGGTTGGCGATGGCCGCCAGCAGCGGGCGGTGATCGATGTCGGGTGCCACTGCCAGGGTCAGGCTGCTCTCCAGCCCTTGGGACAGCTCCAGAGCATGAACCTGCAGCAGCCCCAGTTGTTCGGCAATCAGCCTGGCGTGTGGCTCCAG  >  NC_002947/2567447‑2567732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑