Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,603,228 T→C 9.3% I62T (ATT→ACT)  PP_5513 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,603,2280TC 9.3% 111.2 / 2.4 43I62T (ATT→ACT) PP_5513hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/22);  new base C (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCAGGCCATGACCGACCTTG  >  NC_002947/2603085‑2603371
                                                                                                                                               |                                                                                                                                               
ggaggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTGCTGTACCCCGTCCGGTCGGGTCACTGATTcccc                                                                                                                                            >  8:215432/1‑149 (MQ=255)
 gaggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTcccc                                                                                                                                            <  6:39931/148‑1 (MQ=255)
 gaggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGACTcccc                                                                                                                                            >  7:38553/1‑148 (MQ=255)
 gaggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGACTcccc                                                                                                                                            >  5:174914/1‑148 (MQ=255)
 gaggaAGTGGATGGCGTGGACTACTGGGAATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTcccc                                                                                                                                            <  2:265675/148‑1 (MQ=255)
   ggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGACTCCCCCAt                                                                                                                                         <  6:174914/149‑1 (MQ=255)
   ggaAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGACTCCCCCAt                                                                                                                                         <  8:38553/149‑1 (MQ=255)
     aaGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATgg                                                                                                                                       <  7:110500/149‑1 (MQ=255)
           aTGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGcaca                                                                                                                                 >  2:238588/1‑149 (MQ=255)
                 tGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGc                                                                                                                           <  5:139442/149‑1 (MQ=255)
                        ctGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAggcg                                                                                                                     <  1:238588/148‑1 (MQ=255)
                         tGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAggcgg                                                                                                                    >  5:223615/1‑148 (MQ=255)
                          gggCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAggcggc                                                                                                                   >  5:294223/1‑148 (MQ=255)
                              aTCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATgg                                                                                                                                       <  2:56824/124‑1 (MQ=255)
                                 ttCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTc                                                                                                            >  8:264643/1‑148 (MQ=255)
                                   cGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTc                                                                                                            <  7:264643/146‑1 (MQ=255)
                                           cGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCt                                                                                                 >  6:100654/1‑149 (MQ=255)
                                            gCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCa                                                                                                                         >  5:238043/1‑124 (MQ=255)
                                            gCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCa                                                                                                                         <  6:238043/124‑1 (MQ=255)
                                             cTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCtgt                                                                                               <  5:100654/149‑1 (MQ=255)
                                                   gATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCga                                                                                           <  6:223615/147‑1 (MQ=255)
                                                                        ccGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATg                                                                    <  6:294223/149‑1 (MQ=255)
                                                                            tACGTGGATGTCCCAGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGt                                                                <  3:44243/149‑1 (MQ=255)
                                                                                               ggttCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCAGATACTGCGAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGTTGGCGTGCCTATTCGCATCagacg                                              >  7:257320/3‑144 (MQ=255)
                                                                                                 ttCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGaa                                            >  8:107818/1‑148 (MQ=255)
                                                                                                 ttCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGaa                                            >  2:89383/1‑148 (MQ=255)
                                                                                                      caCTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTCCCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAAt                                      <  7:255546/149‑1 (MQ=255)
                                                                                                      caCTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAAt                                      >  4:183453/1‑149 (MQ=255)
                                                                                                       aCTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAAt                                      <  3:183453/148‑1 (MQ=255)
                                                                                                       aCTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATg                                     <  7:107818/149‑1 (MQ=255)
                                                                                                           aTGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGTGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGAc                                 >  2:8873/1‑149 (MQ=255)
                                                                                                                    cTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCt                          <  1:89383/147‑1 (MQ=255)
                                                                                                                       tACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATGGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTg                        <  7:215432/146‑1 (MQ=255)
                                                                                                                       tACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGaa                                            >  7:331935/1‑126 (MQ=255)
                                                                                                                       tACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGaa                                            <  8:331935/126‑1 (MQ=255)
                                                                                                                           ccgtccgtTCTGGTCACTGATTCCCCCATGGGGCACATACGGACAGGCGGCAATCGTCCATGACTACCTGTGCGAGACACTCACCGTCCAACTGGATGGCGAGCCTATTCGAATCACCCGAAAAGAATGCGACCTGATCTTGCTCCAg                  >  8:159441/1‑148 (MQ=255)
                                                                                                                             gtccgtTCTGGTCACTGATTCCCCCATCGGGCACATACGGCCAGGCGGCAAGCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCAGGcc               <  6:108333/149‑1 (MQ=255)
                                                                                                                              tccgtTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCAGGCCa              <  7:159441/149‑1 (MQ=255)
                                                                                                                                     tGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCa                   <  6:349896/137‑1 (MQ=255)
                                                                                                                                     tGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCa                   >  5:349896/1‑137 (MQ=255)
                                                                                                                                        tCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAAc                                                                          >  5:245594/1‑79 (MQ=255)
                                                                                                                                        tCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAAc                                                                          <  6:245594/79‑1 (MQ=255)
                                                                                                                                          aCTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCAGGCCATGACCGACCTTg  >  4:33839/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCACTGATTCCCCCATGGGGCACATACGGCCAGGCGGCAATCGTCCATGACTACCTGTGCGAGACCCTCACCGTCCAACTGGATGGCGTGCCTATTCGCATCACCCGAAAAGAATGCGACCGGATCTTGCTCCAGGCCATGACCGACCTTG  >  NC_002947/2603085‑2603371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑