Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,651,276 G→C 6.3% intergenic (+41/+29) PP_2323 → / ← aroF‑I hypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,651,2760GC 6.3% 201.4 / 3.5 63intergenic (+41/+29)PP_2323/aroF‑Ihypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (34/25);  new base C (2/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

GCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGC  >  NC_002947/2651134‑2651411
                                                                                                                                              |                                                                                                                                       
gCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCg                                                                                                                                   <  2:128413/149‑1 (MQ=255)
    tGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGt                                                                                                                               >  4:43985/1‑149 (MQ=255)
     ggAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCCCCAGACACAACAAAAAACCCCGGGCAGTGCCCCGCGtt                                                                                                                              >  6:37741/1‑149 (MQ=255)
      gAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCg                                                                                                                                <  7:38099/146‑1 (MQ=255)
       aGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGtttt                                                                                                                            <  8:3424/149‑1 (MQ=255)
       aGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGtttt                                                                                                                            <  3:61977/149‑1 (MQ=255)
       aGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATATGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGtttt                                                                                                                            >  8:240105/1‑149 (MQ=255)
          aCGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCa                                                                                                                         >  6:320119/1‑149 (MQ=255)
          aCGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCACATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTc                                                                                                                          <  1:123786/148‑1 (MQ=255)
              cGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGttttttgttgt                                                                                                                     <  1:178421/149‑7 (MQ=255)
               gAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTccc                                                                                                                    <  5:320119/149‑1 (MQ=255)
                     aTGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATg                                                                                                              >  4:241969/1‑149 (MQ=255)
                     aTGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATg                                                                                                              >  6:121310/1‑149 (MQ=255)
                                    aTCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTa                                                                                                <  5:165151/148‑1 (MQ=255)
                                       ccGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCt                                                                                            >  5:214849/1‑149 (MQ=255)
                                        cGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTg                                                                                           <  6:214849/149‑1 (MQ=255)
                                        cGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTg                                                                                           >  4:265318/1‑149 (MQ=255)
                                              gCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCgg                                                                                        <  3:43985/146‑1 (MQ=255)
                                                  atatGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGgcagca                                                                                   <  3:265318/147‑1 (MQ=255)
                                                    atGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGGTGACGACAGGAACAAGACACAAAAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTACCGCAATGTTCAGCCGCGCTTAAGCTGAGGCAGCACAt                                                                                >  7:54584/1‑148 (MQ=255)
                                                       tgGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCtt                                                                            <  5:121310/149‑1 (MQ=255)
                                                               tACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGacggggc                                                                    >  6:341828/1‑143 (MQ=255)
                                                                  aTGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAAAGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcat                                                                 >  3:238425/1‑149 (MQ=255)
                                                                   tGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcatg                                                                >  3:122492/1‑149 (MQ=255)
                                                                    ggAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcatg                                                                >  2:155476/1‑148 (MQ=255)
                                                                      aGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcatgcat                                                             >  4:159245/1‑149 (MQ=255)
                                                                         gcCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCAtgct                                                          >  7:109525/1‑149 (MQ=255)
                                                                          ccAGCAGCGGCATTCCAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCAATCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATCCAtgctg                                                         >  1:2589/1‑149 (MQ=255)
                                                                           cagcagCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCAtgctgc                                                        >  7:248015/1‑149 (MQ=255)
                                                                            agcagcGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTgcg                                                       >  2:150427/1‑149 (MQ=255)
                                                                              cagcGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCTCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTgcgct                                                     <  7:240105/149‑2 (MQ=255)
                                                                                gcGGCATTCGAGGAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGTCATGCATGCTGCGCAgg                                                   <  2:101342/149‑1 (MQ=255)
                                                                                gcGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAgg                                                   <  3:159245/149‑1 (MQ=255)
                                                                                gcGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAgg                                                   >  4:131268/1‑149 (MQ=255)
                                                                                     aTTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGtttt                                              >  3:285661/1‑149 (MQ=255)
                                                                                      ttCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtc                                             >  5:179211/1‑149 (MQ=255)
                                                                                        cGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtctc                                           >  8:71328/1‑149 (MQ=255)
                                                                                         gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtctc                                           <  8:248015/148‑1 (MQ=255)
                                                                                         gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCg                                          >  4:427/1‑149 (MQ=255)
                                                                                         gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCg                                          >  3:88129/1‑149 (MQ=255)
                                                                                         gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCg                                          <  4:122492/149‑1 (MQ=255)
                                                                                          aGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCgg                                         >  3:112695/1‑149 (MQ=255)
                                                                                               ggCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGcc                                    <  3:241969/149‑1 (MQ=255)
                                                                                                  cTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGAc                                 >  5:289492/1‑149 (MQ=255)
                                                                                                          acgacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTa                                       >  4:280861/1‑135 (MQ=255)
                                                                                                          acgacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTa                                       <  3:280861/135‑1 (MQ=255)
                                                                                                          acgacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGa                          <  4:285661/148‑1 (MQ=255)
                                                                                                          acgacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGa                          <  6:179211/148‑1 (MQ=255)
                                                                                                               aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGa                                  >  4:176021/1‑135 (MQ=255)
                                                                                                               aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGa                                  <  3:176021/135‑1 (MQ=255)
                                                                                                               aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCa                      <  1:138209/147‑1 (MQ=255)
                                                                                                               aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGAGCTTACTCTGCGGCAGCACATCCTTGAGCTTTGCATGCATGCTGCGCAGTGTTTTCTCTGTAGAAGACCAGTCGATGCAgt                    >  8:163806/1‑148 (MQ=255)
                                                                                                                ggCACCAGACACAACAAAAAACGCCGGTCAGGGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGc                   <  7:163806/149‑1 (MQ=255)
                                                                                                                    ccATACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCTCTATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTcc               <  5:37741/149‑1 (MQ=255)
                                                                                                                     cAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCg              <  6:289492/149‑1 (MQ=255)
                                                                                                                        acacAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGa           >  3:293560/1‑149 (MQ=255)
                                                                                                                          acaacaAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGcc                                    <  7:246419/122‑1 (MQ=255)
                                                                                                                          acaacaAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGcc                                    >  8:246419/1‑122 (MQ=255)
                                                                                                                            aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGa       >  6:123512/1‑149 (MQ=255)
                                                                                                                               aaaaaaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGacac    >  2:233064/1‑149 (MQ=255)
                                                                                                                                aaaaaCGCCGGGCACTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACg   >  6:157396/1‑149 (MQ=255)
                                                                                                                                 aaaaCGCCGGGCACTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTAGGTAGCCGACCTGTCGATGCAGGCGTCCGTGATCGACACGc  >  8:128349/1‑149 (MQ=255)
                                                                                                                                  aaaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGc  >  4:198308/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                       
GCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGC  >  NC_002947/2651134‑2651411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑