Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,710,379 A→T 9.1% R320W (AGG→TGG)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,710,3790AT 9.1% 153.4 / 5.8 55R320W (AGG→TGG) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base T (2/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

ACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAGGGAAG  >  NC_002947/2710240‑2710518
                                                                                                                                           |                                                                                                                                           
aCTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGc                                                                                                                                    >  7:323927/1‑149 (MQ=255)
 cTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAACGCCTGGCCTTGGcc                                                                                                                                   <  5:48530/149‑2 (MQ=255)
    ctcggtgggCATATCACCCTCGCCGGTTGCGCCATGGTGATGCTGCACGACGATGCCCACGGGCTGGCGGTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGAATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGaa                                                                                                                                <  8:323927/146‑1 (MQ=255)
      gggtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCGACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGc                                                                                                                              >  6:111091/1‑149 (MQ=255)
        gtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTg                                                                                                                            >  7:245027/1‑149 (MQ=255)
         tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGaa                                                                                                                                <  8:297586/144‑1 (MQ=255)
         tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGaa                                                                                                                                >  7:297586/1‑144 (MQ=255)
          gggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATTGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGtt                                                                                                                          >  8:119300/1‑149 (MQ=255)
          gggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTAGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGtt                                                                                                                          <  1:217951/149‑1 (MQ=255)
           ggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGtt                                                                                                                          <  4:287100/148‑1 (MQ=255)
            gCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTg                                                                                                                         <  5:148612/148‑1 (MQ=255)
            gCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTg                                                                                                                         >  6:148612/1‑148 (MQ=255)
            gCATTTCACCCTTGGCGCTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCAAGGCGTTGCATGAAGCTGttat                                                                                                                        >  4:101152/1‑147 (MQ=255)
               tttCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAACGCCTGGCCTTGGcc                                                                                                                                   >  8:7379/1‑134 (MQ=255)
               tttCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAACGCCTGGCCTTGGcc                                                                                                                                   <  7:7379/135‑2 (MQ=255)
                ttCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAg                                                                                                                    >  7:13628/1‑149 (MQ=255)
                   aCCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATTGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGga                                                                                                                 <  7:119300/149‑1 (MQ=255)
                    cccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                                                                <  3:198349/149‑1 (MQ=255)
                     ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                                                                >  2:55352/1‑148 (MQ=255)
                       ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGtt                                                                                                                                       <  1:201841/123‑1 (MQ=255)
                       ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGtt                                                                                                                                       >  2:201841/1‑123 (MQ=255)
                       ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAACGCCTGGCCTTGGcctgatc                                                                                                                              <  6:302105/132‑7 (MQ=255)
                       ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAACGCCTGGCCTTGGcctgatc                                                                                                                              >  5:302105/1‑126 (MQ=255)
                                   ccATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAt                                                                                                 >  8:264817/1‑149 (MQ=255)
                                     aTGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAttt                                                                                               >  6:286350/1‑149 (MQ=255)
                                        ggagctctgcACGATGTTGCCCACTGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCTGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCg                                                                                            <  8:300643/143‑1 (MQ=255)
                                        gTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTc                                                                                             <  8:245027/148‑1 (MQ=255)
                                        gTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTc                                                                                             <  5:286350/148‑1 (MQ=255)
                                         tGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCgg                                                                                           <  7:134778/149‑1 (MQ=255)
                                         tGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCgg                                                                                           >  1:136135/1‑149 (MQ=255)
                                          gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGc                                                                                          >  2:42789/1‑149 (MQ=255)
                                             gctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGt                                                                                        >  4:339558/1‑148 (MQ=255)
                                              ctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                                                      <  7:264817/149‑1 (MQ=255)
                                               tgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                                                      >  4:358279/1‑148 (MQ=255)
                                               tgcACGATGATGCCCACGGGCTGGCGTTGAACCGAGACGGCGTGCGCGAACGCCGTATTACGGTATTGCAGGAAATAGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCg                                                                                            >  7:300643/1‑142 (MQ=255)
                                                gcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                                                      >  2:272470/1‑147 (MQ=255)
                                                 cACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAg                                                                                   >  8:9943/1‑149 (MQ=255)
                                                                      gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                                                              <  1:55352/149‑1 (MQ=255)
                                                                       cGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCggg                                                             <  8:13628/149‑1 (MQ=255)
                                                                           gAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAgg                                                                          >  6:113078/1‑132 (MQ=255)
                                                                           gAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAgg                                                                          <  5:113078/132‑1 (MQ=255)
                                                                            aaCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                                                              >  3:213708/1‑143 (MQ=255)
                                                                            aaCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                                                              <  4:213708/143‑1 (MQ=255)
                                                                               cgcgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTa                                                     <  1:272470/149‑1 (MQ=255)
                                                                                    cGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGt                                                <  3:358279/149‑1 (MQ=255)
                                                                                              gAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGt                                       >  4:212366/1‑148 (MQ=255)
                                                                                                aCGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCAc                                    <  3:212366/149‑1 (MQ=255)
                                                                                                  gCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                                                      >  3:84532/1‑97 (MQ=255)
                                                                                                  gCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                                                      <  4:84532/97‑1 (MQ=255)
                                                                                                    cGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGt                                >  6:295137/1‑149 (MQ=255)
                                                                                                            ggTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATAtt                        <  2:136135/149‑1 (MQ=255)
                                                                                                            ggTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATAtt                        <  5:295137/149‑1 (MQ=255)
                                                                                                                     ggAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGAccc               >  2:25990/1‑149 (MQ=255)
                                                                                                                        aaTCGATCAGGCAAAGGCCAGGCGTTTGCTGAAGCTGGTGGAAATGGAAGACTAGGGCGTCGATTTTCGGGGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCTTTTGTGCCGTGACCGGTCGAATATTGAATGCCCCTGc            >  4:188481/1‑149 (MQ=255)
                                                                                                                             aTCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAg       <  6:125201/149‑1 (MQ=255)
                                                                                                                                 ggccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAgg      >  2:363063/1‑146 (MQ=255)
                                                                                                                                 ggccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAgg      >  2:368617/1‑146 (MQ=255)
                                                                                                                                 ggccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAgg      <  1:368617/146‑1 (MQ=255)
                                                                                                                                 ggccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAgg      <  1:363063/146‑1 (MQ=255)
                                                                                                                                 ggccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAGGGaa   <  3:339558/149‑1 (MQ=255)
                                                                                                                                  gccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAGGGAAg  >  1:283515/1‑149 (MQ=255)
                                                                                                                                  gccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAGGGAAg  >  3:264547/1‑149 (MQ=255)
                                                                                                                                      aggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCAc                                    >  5:150211/1‑111 (MQ=255)
                                                                                                                                      aggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCAc                                    <  6:150211/111‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAATATTGAATGACCCTGCTTGAGGGAAG  >  NC_002947/2710240‑2710518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑