Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,130 A→G 7.1% M486V (ATG→GTG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,1300AG 7.1% 161.6 / 2.4 56M486V (ATG→GTG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTGGCCTGGG  >  NC_002947/2754991‑2755271
                                                                                                                                           |                                                                                                                                              
tGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGCTCTATGGGCAGTTGATCATCATGGTGGTGTTCCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTGTACCGTGGTCATGGCGCTGt                                                                                                                                       >  2:92801/1‑149 (MQ=255)
  aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTg                                                                                                                                     >  2:339182/1‑149 (MQ=255)
      gtTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcg                                                                                                                                 >  8:255563/1‑149 (MQ=255)
       tttGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcgc                                                                                                                                >  3:350076/1‑149 (MQ=255)
             gCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGTCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatgat                                                                                                                          >  6:169647/1‑149 (MQ=255)
             gCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatgat                                                                                                                          >  3:17210/1‑149 (MQ=255)
              cTGCCCTCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATc                                                                                                                         <  7:344511/149‑1 (MQ=255)
               tGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATcc                                                                                                                        >  4:79296/1‑149 (MQ=255)
                  ccGTGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATTTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCttt                                                                                                                     <  7:229009/149‑1 (MQ=255)
                  ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCttt                                                                                                                     <  2:34220/149‑1 (MQ=255)
                    gcgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑Cg                                                                                                                  <  3:79296/149‑1 (MQ=255)
                        aGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCGTgg                                                                                                                                             <  6:113284/119‑1 (MQ=255)
                        aGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCGTgg                                                                                                                                             <  6:118575/119‑1 (MQ=255)
                        aGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCGTgg                                                                                                                                             >  5:118575/1‑119 (MQ=255)
                        aGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCGTgg                                                                                                                                             >  5:113284/1‑119 (MQ=255)
                         gCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCAc                                                                                                             >  1:197804/1‑149 (MQ=255)
                                 gccgCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACgttcgttc                                                                                                     >  4:314350/1‑149 (MQ=255)
                                  ccgCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTcc                                                                                                    <  1:78223/149‑1 (MQ=255)
                                    gCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCgg                                                                                                  >  8:270562/1‑149 (MQ=255)
                                           tATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCAt                                                                                           >  6:92142/1‑149 (MQ=255)
                                             tGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCga                                                                                                                              >  7:328278/1‑113 (MQ=255)
                                             tGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCga                                                                                                                              <  8:328278/113‑1 (MQ=255)
                                             tGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCAtt                                                                                          <  7:255563/148‑1 (MQ=255)
                                             tGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCAtt                                                                                          <  1:339182/148‑1 (MQ=255)
                                             tGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCAt                                                                                           <  4:364666/147‑1 (MQ=255)
                                              gggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGc                                                                                        >  1:2543/1‑149 (MQ=255)
                                               ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCAcgtt                                                                                                          >  3:25857/1‑130 (MQ=255)
                                               ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCAcgtt                                                                                                          <  4:25857/130‑1 (MQ=255)
                                                 cAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCt                                                                                     <  8:350596/149‑1 (MQ=255)
                                                  aGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTg                                                                                    >  1:226848/1‑149 (MQ=255)
                                                                 tggtgTACCTGCCGATCTTTGTCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAg                                                                     <  5:169647/149‑1 (MQ=255)
                                                                   gtgtACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGt                                                                   <  4:17210/149‑1 (MQ=255)
                                                                          tGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCTATTGCCCTGTTCGTCACCGGCAAGGTCAagg                                                              >  1:313980/1‑147 (MQ=255)
                                                                              gATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagag                                                         >  7:178530/1‑148 (MQ=255)
                                                                                    tGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagaggaa                                                      <  7:21118/145‑1 (MQ=255)
                                                                                    tGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagagg                                                        <  2:2543/143‑1 (MQ=255)
                                                                                      cccTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTg                                                <  2:197804/149‑1 (MQ=255)
                                                                                             cGGGGTCGAGGGCAAGATGTTCGACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTTTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGc                                         <  7:130670/149‑1 (MQ=255)
                                                                                             cGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑Cg                                                                                                                  >  1:230645/1‑76 (MQ=255)
                                                                                             cGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑Cg                                                                                                                  <  2:230645/76‑1 (MQ=255)
                                                                                             cGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGc                                         <  3:314350/149‑1 (MQ=255)
                                                                                               gggTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCg                                        >  8:78293/1‑148 (MQ=255)
                                                                                                 gTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTc                                                                                 >  5:60021/1‑105 (MQ=255)
                                                                                                 gTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTc                                                                                 <  6:60021/105‑1 (MQ=255)
                                                                                                 gTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTac                                     <  8:190791/149‑1 (MQ=255)
                                                                                                      gggCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTacggcac                                >  5:265517/1‑149 (MQ=255)
                                                                                                         cAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGt                                              <  8:233733/132‑1 (MQ=255)
                                                                                                         cAAGATGTTCCACCCTATGGCCGTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGt                                              >  7:233733/1‑132 (MQ=255)
                                                                                                            gATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAgc                          >  1:231152/1‑149 (MQ=255)
                                                                                                               gTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTACGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTGGTCCCCGGCAAGGTAAAGGAAGAGGAAGGCCTGGTGATCCGTACGGCACGGAAgcgg                        >  1:100969/1‑148 (MQ=255)
                                                                                                                   cACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGc                   >  6:198145/1‑149 (MQ=255)
                                                                                                                            gCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCt          <  2:100969/149‑1 (MQ=255)
                                                                                                                             ccTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTg         <  7:270562/149‑1 (MQ=255)
                                                                                                                                 tACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTGGc       <  8:178530/147‑1 (MQ=255)
                                                                                                                                      tGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGACGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTGGCCTggg  <  8:57135/147‑1 (MQ=255)
                                                                                                                                      tGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTGGCCTggg  >  7:57135/1‑147 (MQ=255)
                                                                                                                                           |                                                                                                                                              
TGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTGACCGGGGTCGAGGGCAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTT‑CGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGGCACGGCAGCGCTATGCCCCGGTTCTGGCCTGGG  >  NC_002947/2754991‑2755271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑