Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,759,048 A→C 8.3% L39R (CTG→CGG)  PP_2412 ← ParA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,759,0480AC 8.3% 141.7 / 4.2 48L39R (CTG→CGG) PP_2412ParA family protein
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base C (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTAT  >  NC_002947/2758903‑2759196
                                                                                                                                                  |                                                                                                                                                    
ggTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCa                                                                                                                                                   <  6:377672/149‑1 (MQ=255)
  tGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTTGCTGGTCGACCTCGACCCGCag                                                                                                                                                  <  8:40237/149‑2 (MQ=255)
  tGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAg                                                                                                                                                 <  5:45433/149‑1 (MQ=255)
  tGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAg                                                                                                                                                 <  3:227844/149‑1 (MQ=255)
        ccACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCgg                                                                                                                                           <  4:190273/149‑1 (MQ=255)
              ggTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGccc                                                                                                                                     >  2:343442/1‑149 (MQ=255)
                   ttcttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCTGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTcggc                                                                                                                                >  3:93173/1‑149 (MQ=255)
                           ggcggcggTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTgg                                                                                                                        <  1:249474/149‑1 (MQ=255)
                               gcggTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAgc                                                                                                                    <  4:17177/149‑1 (MQ=255)
                                    aaCCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGATCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAgcggc                                                                                                                 >  1:27928/1‑147 (MQ=255)
                                     aCCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGAGCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGGCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAg                                                                                                              <  5:96016/149‑1 (MQ=255)
                                      ccGGCGACAAGGTCAGGCCGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGCTAGAAGGTGGCATTCGCC‑TGCGGGTCGAGGTCGACCAGCAAGGATCGGTAGCCCTCGGCCGCGCTGGCAGCCGCGacg                                                                                                             >  8:179892/1‑147 (MQ=255)
                                              aaGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGc                                                                                                     <  6:13281/149‑1 (MQ=255)
                                              aaGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGc                                                                                                     >  7:334430/1‑149 (MQ=255)
                                              aaGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGc                                                                                                     >  8:166432/1‑149 (MQ=255)
                                                  tCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGACGGCGCTGGCAGCGGCGAGGTTGCAGGCGATg                                                                                                 >  5:62425/1‑149 (MQ=255)
                                                       cGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGa                                                                                            <  7:277965/149‑1 (MQ=255)
                                                          aagaagTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGATCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGAttt                                                                                         <  2:27928/149‑1 (MQ=255)
                                                                  ggCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGc                                                                                 >  3:164448/1‑149 (MQ=255)
                                                                  ggCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGAAGGCGATGCTGGATTTGCCGACGc                                                                                 >  3:169951/1‑149 (MQ=255)
                                                                         ccGGGTGGAATGGTGTCGTTGAGAAGCGCGGTGAGGTAGAAGGTGGGATTGGCC‑TGCGGGTCGAGGTCGACCAGAAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGATGTAGCAGGCGATGCTGTATTTGCCGACGCCACAtt                                                                           >  7:56601/1‑148 (MQ=255)
                                                                          cGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTc                                                                         <  1:343442/149‑1 (MQ=255)
                                                                          cGGGTGGAATGGTGTCGTTGACCAGCCCGGGGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGGCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTc                                                                         <  1:216731/149‑1 (MQ=255)
                                                                           gggTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGACGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCt                                                                        <  6:62425/149‑1 (MQ=255)
                                                                                          gTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCacac                                                         <  7:166432/149‑1 (MQ=255)
                                                                                                cAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCa                                                                               >  8:227701/1‑121 (MQ=255)
                                                                                                cAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCa                                                                               <  7:227701/121‑1 (MQ=255)
                                                                                                   cccGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCAt                                                  >  2:48524/1‑147 (MQ=255)
                                                                                                       gTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGTCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACg                                            <  4:93173/149‑1 (MQ=255)
                                                                                                        tGAGGTAGACCGGGGCATTGGCC‑GGCGTGTCGACGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCTGCGGCGAGGTTTCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGc                                           <  8:334430/149‑1 (MQ=255)
                                                                                                              aGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATc                                      >  5:124427/1‑148 (MQ=255)
                                                                                                                            gCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATg                       >  7:160120/1‑149 (MQ=255)
                                                                                                                              c‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGt                      >  7:100872/1‑148 (MQ=255)
                                                                                                                              c‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTc                     <  4:169951/149‑1 (MQ=255)
                                                                                                                              c‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTc                     <  4:164448/149‑1 (MQ=255)
                                                                                                                               ttGCTGGTCGACCTCGACCCGCAAGGTTCGGTAGCCCTCGGCGACGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACATTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGt                      >  4:244214/2‑148 (MQ=255)
                                                                                                                                  ggggTCGAGTTCTACCAGGAAGGTTCGGTAGCCCTCGGCGGCTCTGGCAGCGGCGAGGTTGCAGGCTATGGTGGATTTGCCGACTCCACCTTTCTGTTTGAATACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCa                    <  7:179892/146‑1 (MQ=255)
                                                                                                                                   tggtcgacctcgaCCCGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGaaaa                                                               >  4:48470/2‑103 (MQ=255)
                                                                                                                                   tggtcgacctcgaCCCGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGaaaa                                                               <  3:48470/102‑1 (MQ=255)
                                                                                                                                     gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCg               >  1:365523/1‑149 (MQ=255)
                                                                                                                                     gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCg               >  5:168556/1‑149 (MQ=255)
                                                                                                                                            ccgaCCAGCAAGGTTCGGCACCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCaa        <  8:56601/148‑1 (MQ=255)
                                                                                                                                              gaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATc      >  3:68910/1‑149 (MQ=255)
                                                                                                                                              gaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATc      <  1:48524/149‑1 (MQ=255)
                                                                                                                                                 cAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                                >  2:296849/1‑104 (MQ=255)
                                                                                                                                                 cAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                                <  1:296849/104‑1 (MQ=255)
                                                                                                                                                 cAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTa   <  8:160120/149‑1 (MQ=255)
                                                                                                                                                 cAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTa   >  5:249429/1‑149 (MQ=255)
                                                                                                                                                  aGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTAt  <  6:124427/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCC‑TGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTAT  >  NC_002947/2758903‑2759196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑