Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,762,980 C→A 9.2% G311C (GGC→TGC) ‡ PP_2417 ← iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,762,9800CA 9.2% 187.2 / 9.5 65G311C (GGC→TGC) ‡PP_2417iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base A (3/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-02

GCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAAC  >  NC_002947/2762837‑2763117
                                                                                                                                               |                                                                                                                                          
gCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTg                                                                                                                                        <  4:362845/148‑1 (MQ=255)
  agccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATc                                                                                                                                     <  8:108401/149‑1 (MQ=255)
  agccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATc                                                                                                                                     >  3:364270/1‑149 (MQ=255)
  agccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCGGCCAGGTGATc                                                                                                                                     <  8:39585/149‑1 (MQ=255)
   gccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCa                                                                                                                                    <  4:104208/149‑1 (MQ=255)
     cccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCCGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGc                                                                                                                                  >  3:161138/1‑149 (MQ=255)
      ccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCg                                                                                                                                 >  7:169510/1‑149 (MQ=255)
      ccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCg                                                                                                                                 >  6:352389/1‑149 (MQ=255)
        agcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCTCCTGGCCGGTGATCAGCGTg                                                                                                                               >  8:120280/1‑149 (MQ=255)
          cccATGGCAGGCCAGCGGAGGGATGCGAACAGATGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTgcg                                                                                                                             >  2:26356/1‑149 (MQ=255)
                gCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAg                                                                                                                       <  2:167152/149‑1 (MQ=255)
                  aGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGaa                                                                                                                     >  1:11378/1‑149 (MQ=255)
                   ggCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAAt                                                                                                                    <  6:300004/149‑1 (MQ=255)
                         cGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGc                                                                                                              <  8:169510/149‑1 (MQ=255)
                                aTGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAAt                                                                                                        >  4:247273/1‑148 (MQ=255)
                                  gCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAg                                                                                                     >  5:339048/1‑149 (MQ=255)
                                   cGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAgg                                                                                                    <  6:286393/149‑1 (MQ=255)
                                   cGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAgg                                                                                                    <  4:364270/149‑1 (MQ=255)
                                    gAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAgg                                                                                                    >  8:36974/1‑148 (MQ=255)
                                     aaCAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGaa                                                                                                  <  7:74079/149‑1 (MQ=255)
                                       cAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACa                                                                                                <  8:43/149‑1 (MQ=255)
                                           tGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAgcg                                                                                             >  8:302207/1‑148 (MQ=255)
                                             ccATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCAc                                                                                          >  7:214834/1‑149 (MQ=255)
                                               aTGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCActg                                                                                        >  5:155196/1‑147 (MQ=255)
                                                   cGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCa                                                                                    <  8:292098/149‑1 (MQ=255)
                                                         tGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTGTGCCACAATCAGGAACAGCGCACTGGGCGGTgc‑gc                                                                             <  1:26356/149‑1 (MQ=255)
                                                         tGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACTGGGCGGTgc‑gc                                                                             <  6:155196/149‑1 (MQ=255)
                                                         tGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTgc‑gc                                                                             >  5:334119/1‑149 (MQ=255)
                                                           ggCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCt                                                                            <  7:36974/148‑1 (MQ=255)
                                                             cgcCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACTGGGCGGTGC‑GCTgtt                                                                         <  7:120280/149‑3 (MQ=255)
                                                                    gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTgc                                                                                >  1:93092/1‑136 (MQ=255)
                                                                    gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTgc                                                                                <  2:93092/136‑1 (MQ=255)
                                                                    gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCaa                                                                   >  8:214303/1‑148 (MQ=255)
                                                                     aCGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACa                                                                 <  6:334119/149‑1 (MQ=255)
                                                                        gCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCCCCTGGCCGCTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGAc                                                              >  7:126698/1‑149 (MQ=255)
                                                                          tGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACgg                                                            >  2:47040/1‑149 (MQ=255)
                                                                           gACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGc                                                           >  7:347239/1‑149 (MQ=255)
                                                                            aCCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCt                                                          >  7:123884/1‑149 (MQ=255)
                                                                                gAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGc                                                      >  2:49296/1‑149 (MQ=255)
                                                                                  aTCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGccc                                                    >  3:209228/1‑149 (MQ=255)
                                                                                       tGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGc                                               <  8:123884/149‑1 (MQ=255)
                                                                                       tGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGc                                               >  5:214934/1‑149 (MQ=255)
                                                                                         cGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGCAGCTGGCGGGCAACAGACGGCTGTGCCCGGGGcc                                              <  1:49296/149‑1 (MQ=255)
                                                                                                 ggggCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAg                                     <  7:214303/149‑1 (MQ=255)
                                                                                                    gCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGgcg                                  <  8:347239/149‑1 (MQ=255)
                                                                                                      aCCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGgcgc                                 >  6:350609/1‑148 (MQ=255)
                                                                                                      aCCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGA‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCa                                >  3:44648/1‑149 (MQ=255)
                                                                                                       ccGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCaa                               <  3:247273/149‑1 (MQ=255)
                                                                                                        cGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAg                              <  1:47040/149‑1 (MQ=255)
                                                                                                              gcgcCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgg                        <  6:214934/149‑1 (MQ=255)
                                                                                                               cgcCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAGGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATggg                       >  3:266455/1‑149 (MQ=255)
                                                                                                                gcCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgggg                      <  6:339048/149‑1 (MQ=255)
                                                                                                                gcCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgggg                      <  8:214834/149‑1 (MQ=255)
                                                                                                                       ccACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgggg                      >  8:46351/1‑142 (MQ=255)
                                                                                                                       ccACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgggg                      <  7:46351/142‑1 (MQ=255)
                                                                                                                       ccACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAAc                >  3:273762/1‑148 (MQ=255)
                                                                                                                        cACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCa              <  5:352389/149‑1 (MQ=255)
                                                                                                                            cccACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGccc          <  7:302207/149‑1 (MQ=255)
                                                                                                                             ccACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGccc          >  3:153414/1‑148 (MQ=255)
                                                                                                                                   tgATCACCGGCCAGGTGATCCGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCtgt                                                        <  2:315629/95‑1 (MQ=255)
                                                                                                                                   tgATCACCGGCCAGGTGATCCGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCtgt                                                        >  1:315629/2‑96 (MQ=255)
                                                                                                                                   tgATCACCGGCCAGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACaaaa   >  5:54393/2‑149 (MQ=255)
                                                                                                                                   tgATCACCGGCCAGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACaaaa   >  1:44459/2‑149 (MQ=255)
                                                                                                                                     aTCACCGGCCAGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAAc  <  5:77382/148‑1 (MQ=255)
                                                                                                                                          cTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACgg                                                            <  6:169982/85‑1 (MQ=255)
                                                                                                                                               cGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGgcg                                  >  4:30679/1‑106 (MQ=255)
                                                                                                                                               cGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGgcg                                  <  3:30679/106‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
GCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGAACAGCGCACCGCCCAGTGC‑GCTGGCGGGCAACAGACGGCTGTGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAAC  >  NC_002947/2762837‑2763117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑