Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,764,865 G→C 8.4% A46G (GCC→GGC)  PP_2418 ← cobalamin ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,764,8650GC 8.4% 141.7 / 2.6 46A46G (GCC→GGC) PP_2418cobalamin ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (23/19);  new base C (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCCAGCTTGTCACCCAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGC  >  NC_002947/2764718‑2765008
                                                                                                                                                   |                                                                                                                                               
cccGCCAGCTTGTCACCCAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTggg                                                                                                                                                 <  6:78261/148‑1 (MQ=255)
cccGCCAGCTTGTCACCCAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGggc                                                                                                                                                <  5:330226/149‑1 (MQ=255)
  cGCCAGCTTGTCACCCAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGggcca                                                                                                                                              <  3:273379/149‑1 (MQ=255)
       gCTTGTCACCAAGCCCCAGGGCATAGAGCAGCTCGGTACCGCCCAGGCCGATGGTGACGGCGCGCTGCCTCGCCTGGGAAAAAACCTGCGGCTTGCCGCAGTTGTCGACAGTCAGCGGGTAGTGGGTGGCAACGGCCTGgtccaggc                                                                                                                                           >  5:74864/1‑147 (MQ=255)
                ccAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCt                                                                                                                                 >  7:244599/1‑148 (MQ=255)
                ccAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCt                                                                                                                                 >  1:5068/1‑148 (MQ=255)
                 cAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTa                                                                                                                               <  1:156422/149‑1 (MQ=255)
                  aGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGTGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAg                                                                                                                              >  8:240599/1‑149 (MQ=255)
                  aGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAg                                                                                                                              >  8:33950/1‑149 (MQ=255)
                      ccAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGggccaggc                                                                                                                                           >  2:233352/1‑132 (MQ=255)
                      ccAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGggccaggc                                                                                                                                           <  1:233352/132‑1 (MQ=255)
                       cAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCcg                                                                                                                         >  4:127258/1‑149 (MQ=255)
                                  gcagcTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGGCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTg                                                                                                              <  6:158840/149‑1 (MQ=255)
                                      cTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAg                                                                                                                              >  1:231148/1‑129 (MQ=255)
                                      cTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAg                                                                                                                              <  2:231148/129‑1 (MQ=255)
                                          gTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACg                                                                                                      >  3:142728/1‑149 (MQ=255)
                                           tACCGGCCTGGCCGATGGTGACGGTGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACgg                                                                                                     <  7:240599/149‑1 (MQ=255)
                                             ccGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGag                                                                                                   >  7:107340/1‑149 (MQ=255)
                                             ccGGCCTGGCCGAGGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGa                                                                                                    <  5:202807/148‑1 (MQ=255)
                                              cGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGag                                                                                                   >  7:291135/1‑148 (MQ=255)
                                                 ccTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGagcagc                                                                                               <  6:93736/149‑1 (MQ=255)
                                                                cGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGAtctc                                                                                <  3:127258/149‑1 (MQ=255)
                                                                  gcgcgcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCt                                                                              <  2:74609/149‑1 (MQ=255)
                                                                    gcgcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTAt                                                                            >  6:272097/1‑149 (MQ=255)
                                                                     cgcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATc                                                                           <  6:47433/149‑1 (MQ=255)
                                                                      gcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCg                                                                          >  1:133734/1‑149 (MQ=255)
                                                                      gcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCg                                                                          >  2:169643/1‑149 (MQ=255)
                                                                      gcTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCg                                                                          <  4:142728/149‑1 (MQ=255)
                                                                              gcCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTc                                                                  >  3:164350/1‑149 (MQ=255)
                                                                                     cAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAg                                                           <  8:244599/149‑1 (MQ=255)
                                                                                     cAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTGCTGGTGAg                                                           >  8:192759/1‑149 (MQ=255)
                                                                                      aaaaaCCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTCAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAg                                                           >  3:125689/1‑148 (MQ=255)
                                                                                       aaaaCCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGa                                                         <  8:291135/149‑1 (MQ=255)
                                                                                               cGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCAc                                                   <  4:164350/147‑1 (MQ=255)
                                                                                               cGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCAc                                                   <  7:197251/147‑1 (MQ=255)
                                                                                                 gCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGa                                                >  5:177801/1‑148 (MQ=255)
                                                                                                   ttGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTgg                                                                                        <  1:78742/106‑1 (MQ=255)
                                                                                                   ttGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTgg                                                                                        >  2:78742/1‑106 (MQ=255)
                                                                                                   ttGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGAc                                               >  1:288302/1‑147 (MQ=255)
                                                                                                       cGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCAt                                         >  3:339600/1‑149 (MQ=255)
                                                                                                               tCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCGGAGc                                 <  7:33950/149‑1 (MQ=255)
                                                                                                                 gACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGccc                               <  4:339600/149‑1 (MQ=255)
                                                                                                                 gACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGccc                               <  5:152581/149‑1 (MQ=255)
                                                                                                                    cGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACgg                                                 >  3:74462/1‑128 (MQ=255)
                                                                                                                    cGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACgg                                                 <  4:74462/128‑1 (MQ=255)
                                                                                                                           gggTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGccacccg                     >  7:208943/1‑143 (MQ=255)
                                                                                                                                    gtggCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCaa            >  2:270886/1‑149 (MQ=255)
                                                                                                                                        cactggCCTGGCCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTgggg               <  7:219212/138‑1 (MQ=255)
                                                                                                                                        cactggCCTGGCCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTgggg               >  8:219212/5‑142 (MQ=255)
                                                                                                                                              ccTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGc  >  3:48747/1‑149 (MQ=255)
                                                                                                                                               cTGGCCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGa                                                            >  2:304626/1‑90 (MQ=255)
                                                                                                                                               cTGGCCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGa                                                            <  1:304626/90‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCCGCCAGCTTGTCACCCAGCCCCAGGGCATAGAGCAGCTCGGTACCGGCCTGGCCGATGGTGACGGCGCGCTGCGGCGCCTGGGCAAAAACCTGCGGCTTGCCGCAGTTGTCGACCGTCAGCGGGTAGTGGGTGGCAGCGGCCTGGGCCAGGCCAGTGAGGCTTAGGCCCGCGAGCAGGGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGC  >  NC_002947/2764718‑2765008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑