Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,786,143 G→T 8.7% L176I (CTC→ATC)  alx ← alkaline‑induced transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,786,1430GT 8.7% 143.4 / 3.6 46L176I (CTC→ATC) alxalkaline‑induced transporter
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

AGGTCGGCCAGTTCGATCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGC  >  NC_002947/2785995‑2786287
                                                                                                                                                    |                                                                                                                                                
aGGTCGGCCAGTTCGATCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAg                                                                                                                                                  <  5:176164/149‑1 (MQ=255)
       ccAGTTCGATCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCggg                                                                                                                                           >  5:197634/1‑149 (MQ=255)
             cGATCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGgttgtt                                                                                                                                      <  5:328505/148‑1 (MQ=255)
                tCAGTACCAGTGCCAGAAACAGCGGGGTGGNATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGc                                                                                                                                   >  1:34897/1‑148 (MQ=255)
                tCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGcc                                                                                                                                  <  4:92247/149‑1 (MQ=255)
                tCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGc                                                                                                                                   >  1:10224/1‑148 (MQ=255)
                   gTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGGCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAgg                                                                                                                               >  4:194454/1‑149 (MQ=255)
                        aGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTgg                                                                                                                          <  5:114874/149‑1 (MQ=255)
                            ccAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGa                                                                                                                      >  1:226924/1‑149 (MQ=255)
                              aGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACt                                                                                                                    <  8:152887/149‑1 (MQ=255)
                              aGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACt                                                                                                                    >  8:142540/1‑149 (MQ=255)
                              aGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACt                                                                                                                    >  6:141773/1‑149 (MQ=255)
                                 aaCAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTc                                                                                                                 <  6:197634/149‑1 (MQ=255)
                                          gTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATCGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGa                                                                                                        >  4:1629/1‑149 (MQ=255)
                                           tGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGa                                                                                                        >  1:223674/1‑148 (MQ=255)
                                                     cAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTCGTCGCTAACCCGCATGTGCCGCAGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGCGCGACTGTCTTCCTTCGACAGCGCCAttt                                                                                             >  3:279555/1‑149 (MQ=255)
                                                      aGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCAtttt                                                                                            >  4:111134/1‑149 (MQ=255)
                                                      aGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCAtttt                                                                                            <  2:10224/149‑1 (MQ=255)
                                                            ttGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCAcccc                                                                                      >  3:159919/1‑149 (MQ=255)
                                                                   gCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCa                                                                                                                                 >  7:115314/1‑99 (MQ=255)
                                                                   gCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCa                                                                                                                                 <  8:115314/99‑1 (MQ=255)
                                                                   gCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAc                                                                               <  7:349834/149‑1 (MQ=255)
                                                                    cccGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAc                                                                               <  3:111134/148‑1 (MQ=255)
                                                                       ggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           <  5:141773/149‑1 (MQ=255)
                                                                       ggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           <  4:159919/149‑1 (MQ=255)
                                                                       ggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           <  2:34897/149‑1 (MQ=255)
                                                                           ggggTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAgg                                                                         >  5:206607/1‑147 (MQ=255)
                                                                           ggggTATGGAGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGaa                                                                       <  2:226924/149‑1 (MQ=255)
                                                                                        cGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGAt                                                           <  7:142540/148‑1 (MQ=255)
                                                                                         taagaagTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGATGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           <  4:284248/130‑1 (MQ=255)
                                                                                         gaagaaGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGATGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           <  4:279341/131‑1 (MQ=255)
                                                                                         gaagaaGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGATGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           >  3:279341/1‑131 (MQ=255)
                                                                                         gaagaaGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGATGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAAcagca                                                                           >  3:284248/1‑131 (MQ=255)
                                                                                           agaagTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACTAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTaa                                                       <  8:247329/149‑1 (MQ=255)
                                                                                           agaagTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTa                                                        >  5:93989/1‑148 (MQ=255)
                                                                                                     gCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAgg                                             >  6:338887/1‑149 (MQ=255)
                                                                                                               tGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTg                                   >  8:326952/1‑149 (MQ=255)
                                                                                                                 gTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCATAGTTCTg                                   <  6:73604/147‑1 (MQ=255)
                                                                                                                 gTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGtt                                      <  7:186398/144‑1 (MQ=255)
                                                                                                                    ggTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCa                              <  6:93989/149‑1 (MQ=255)
                                                                                                                          ccGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAgcgccg                        >  3:190852/1‑149 (MQ=255)
                                                                                                                                   ccgccgCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGc               <  6:206607/149‑1 (MQ=255)
                                                                                                                                     gccgcACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCA‑CCAGGGAAACGTCTGTACCAGCGCCGCCCCCACGCCg             >  4:186653/1‑148 (MQ=255)
                                                                                                                                      ccgcACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGa            <  3:1629/149‑1 (MQ=255)
                                                                                                                                        gcACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCCCCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCACCGCCGCGCCCACGCCGAt           >  5:285595/1‑148 (MQ=255)
                                                                                                                                        gcACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATc          <  3:194454/149‑1 (MQ=255)
                                                                                                                                                ttGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACCCCGATCATGATGGc  >  3:90741/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGGTCGGCCAGTTCGATCAGTACCAGTGCCAGAAACAGCGGGGTGGCATAACGCAGCGCCTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGC  >  NC_002947/2785995‑2786287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑