Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,796,837 A→T 8.6% intergenic (+21/‑111) PP_t43 → / → PP_2449 tRNA‑Val/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,796,8370AT 8.6% 167.8 / 5.9 58intergenic (+21/‑111)PP_t43/PP_2449tRNA‑Val/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base T (3/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

AAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAG  >  NC_002947/2796690‑2796975
                                                                                                                                                   |                                                                                                                                          
aaaGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGa                                                                                                                                            >  5:188872/1‑148 (MQ=255)
  agagCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAAt                                                                                                                                         <  1:5012/149‑1 (MQ=255)
        ttACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCCCATAGCTCAGTTGGTTAGAGCACGACCTTGACAAGGAGGGGGTCGTTGGTTCGAGTCGATTTGTGCGTACCAAATCGAAAAAGGGCGGTGAGAATTGAGCgg                                                                                                                                   >  7:238924/1‑148 (MQ=17)
             ggTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCATTTCTGATCGCCCctt                                                                                                                              <  8:265317/149‑1 (MQ=17)
               tACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCttttt                                                                                                                            <  5:283119/149‑1 (MQ=255)
                        ggCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCATTTCTGATCGCCCTTTTTCGATTTggt                                                                                                                   <  2:172082/149‑3 (MQ=17)
                          ccACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGcc                                                                                                                 >  8:123734/1‑149 (MQ=255)
                            aCTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCttttt                                                                                                                            <  8:177755/136‑1 (MQ=255)
                            aCTAATATGCGCAACACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCttttt                                                                                                                            >  7:177755/1‑136 (MQ=255)
                              tAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCg                                                                                                               >  3:280669/1‑147 (MQ=255)
                                   tGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAagtagc                                                                                                        <  7:270371/149‑1 (MQ=255)
                                      gcACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCgtg                                                                                                     >  6:1483/1‑149 (MQ=255)
                                      gcACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCgtg                                                                                                     >  6:313067/1‑149 (MQ=255)
                                          cacaCGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAAc                                                                                                 <  5:1483/149‑1 (MQ=255)
                                              cGACAGGCACATAGCTCAGTTGGTTAGATCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAATGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGttt                                                                                             <  7:327655/149‑1 (MQ=255)
                                              cGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGttt                                                                                             <  2:268793/149‑1 (MQ=255)
                                              cGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGttt                                                                                             <  6:188872/149‑1 (MQ=255)
                                              agACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGTGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAATGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGttt                                                                                             <  7:174340/148‑1 (MQ=255)
                                               gACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTg                                                                                            >  5:201637/1‑149 (MQ=255)
                                                    gCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcgc                                                                                       >  1:146207/1‑149 (MQ=255)
                                                    gCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcgc                                                                                       <  7:123734/149‑1 (MQ=255)
                                                           gCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCgg                                                                                <  4:280669/149‑1 (MQ=255)
                                                            cTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCggg                                                                               <  3:167198/149‑1 (MQ=255)
                                                            cTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCggg                                                                               >  4:46406/1‑149 (MQ=255)
                                                             ctaGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGATTTGATCGCCCTTTTTGCATTTGCGCCCGAAGGAGCGTGTAACGTTTGGTCGCGGTCCggg                                                                               <  1:97762/146‑1 (MQ=255)
                                                               aGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTTGTTCGAGTACAATTGTGACTACCAAATCGAAAAAGGGCGAGCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCAt                                                                            >  8:11246/1‑149 (MQ=255)
                                                                gtaggTTAGAGCACCACCTTGACAAGGTGGGGGTCGTTGGTTCGAGACCAATAGTGCCTACCAAATCCAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGCTCGCGGTCCcgg                                                                               >  2:97762/4‑145 (MQ=25)
                                                                 ttGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATAc                                                                          >  4:296051/1‑149 (MQ=255)
                                                                        gagCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGc                                                                    <  5:313067/148‑1 (MQ=255)
                                                                           caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcg                                                                                        <  2:99857/125‑1 (MQ=255)
                                                                           caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTcg                                                                                        >  1:99857/1‑125 (MQ=255)
                                                                            accaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAagcagc                                                                                                        <  7:338247/108‑1 (MQ=255)
                                                                            accaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAagcagc                                                                                                        >  8:338247/1‑108 (MQ=255)
                                                                             ccaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTa                                                              <  6:4205/149‑1 (MQ=255)
                                                                                          gtggGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCtt                                                 <  3:296051/149‑1 (MQ=255)
                                                                                               ggTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAAt                                            >  6:367441/1‑149 (MQ=255)
                                                                                               ggTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCATTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGCCGTGCTAGAATCCGCCCCTTCGaa                                             >  4:45512/1‑148 (MQ=255)
                                                                                                     tGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCg                                                       <  1:238508/132‑1 (MQ=255)
                                                                                                     tGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCg                                                       >  2:238508/1‑132 (MQ=255)
                                                                                                       gTTCGAGTCCAATTGTGCCTACCAAATCGATAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAgg                                     >  1:33604/1‑148 (MQ=255)
                                                                                                       gTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGtt                                                                                              >  4:330153/1‑91 (MQ=255)
                                                                                                       gTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGtt                                                                                              <  3:330153/91‑1 (MQ=255)
                                                                                                         tCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTa                                                              >  5:266763/1‑121 (MQ=255)
                                                                                                         tCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTa                                                              <  6:266763/121‑1 (MQ=255)
                                                                                                              tCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTc                                                <  1:155654/130‑1 (MQ=255)
                                                                                                              tCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTc                                                >  2:155654/1‑130 (MQ=255)
                                                                                                              tCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACAcgcg                             >  1:30571/1‑149 (MQ=255)
                                                                                                              tCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACAcgcg                             >  8:320158/1‑149 (MQ=255)
                                                                                                                       gCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATAccc                                                                        <  6:6202/97‑1 (MQ=255)
                                                                                                                       gCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATAccc                                                                        >  5:6202/1‑97 (MQ=255)
                                                                                                                        ccTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAAc                    <  6:201637/148‑1 (MQ=255)
                                                                                                                         cTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTg                  >  8:190051/1‑149 (MQ=255)
                                                                                                                          tACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTgg                 >  4:121177/1‑149 (MQ=255)
                                                                                                                          tACCAAATCGAAAAAGGGCGATAAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTgg                 >  6:303149/1‑149 (MQ=255)
                                                                                                                             cAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCtg              >  1:169647/1‑149 (MQ=255)
                                                                                                                                    aaaaaGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAAt       >  4:323370/1‑149 (MQ=255)
                                                                                                                                     aaaaGGGCGATCATTTCTGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATg      >  7:262427/1‑149 (MQ=255)
                                                                                                                                      aaaGGGCGATCATTTCTGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATgg     >  6:162622/1‑149 (MQ=255)
                                                                                                                                         gggCGATCATTTCTGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAg  >  4:151833/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
AAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCGATCAGAAATGATCGCCCTTTTTGCATTTGCGCCCGAAGCAGCGTGTAACGTTTGGTCGCGGTCCGGGCATACCCCGGCGTGCTAGAATCCGCCCCTTCGAATCTGGAGGTACACGCGTGCGCAAACTGGCTGTGGTAATGGCAG  >  NC_002947/2796690‑2796975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑