Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	233,846	A→G	10.1%	intergenic (+34/+29)	PP_0180 → / ← PP_0181	cytochrome c family protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	233,846	0	A	G	10.1%	155.7 / 6.5	59	intergenic (+34/+29)	PP_0180/PP_0181	cytochrome c family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base G (3/3);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCTTCTCATGCAG  >  NC_002947/233699‑233994                                                                                                                                                    |                                                                                                                                                      gggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCaa                                                                                                                                                       <  4:342971/148‑1 (MQ=255)    aTTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtc                                                                                                                                                   >  4:175850/1‑149 (MQ=255)     ttCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtct                                                                                                                                                  <  1:186813/149‑1 (MQ=255)       cATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtctc                                                                                                                                                 <  7:151033/148‑1 (MQ=255)           ccGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATc                                                                                                                                            <  6:292482/149‑1 (MQ=255)           ccGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATc                                                                                                                                            <  1:73899/149‑1 (MQ=255)            cGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCt                                                                                                                                           >  7:249305/1‑149 (MQ=255)            cGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGATCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGCGCTCAGATCt                                                                                                                                           >  6:281069/1‑149 (MQ=255)                   tACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCATGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCCCCCCAGGTCTCAGATCTTGCGCaa                                                                                                                                    >  2:108539/1‑149 (MQ=255)                     cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGc                                                                                                                                  <  3:175850/149‑1 (MQ=255)                     cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGc                                                                                                                                  <  4:242555/149‑1 (MQ=255)                      tCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCCCACGGGCCGCGCAAGGGGTCAGATCATTCGCaaac                                                                                                                                  >  2:346162/1‑146 (MQ=255)                        gCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCt                                                                                                                                <  8:249305/148‑1 (MQ=255)                         cTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGa                                                                                                                              <  2:264117/149‑1 (MQ=255)                          tGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGAt                                                                                                                             <  8:142964/149‑1 (MQ=255)                                      cGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGAGCATCAggc‑ttgc                                                                                                                <  4:224202/149‑1 (MQ=255)                                         tGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCCGTACCACAGGGACCCCGCAAGGGCTCAGATCTTGCGCAAGCCTGAAGCTCAGGC‑TGGCggt                                                                                                             >  4:312133/1‑149 (MQ=255)                                             cTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtctc                                                                                                                                                 <  3:205765/110‑1 (MQ=255)                                                   ggCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCtt                                                                                                     >  1:232093/1‑147 (MQ=255)                                                    gCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTga                                                                                                   <  3:312133/148‑1 (MQ=255)                                                     ccATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAggc‑tgg                                                                                                                 <  2:230254/133‑1 (MQ=255)                                                     ccATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAggc‑tgg                                                                                                                 >  1:230254/1‑133 (MQ=255)                                                        tCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATg                                                                                              >  1:241176/1‑149 (MQ=255)                                                          gtgtTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGtt                                                                                            <  1:362220/149‑1 (MQ=255)                                                            gtTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCt                                                                                          <  7:224028/149‑1 (MQ=255)                                                            gtTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAACGGCTCAGAAATTGCGCAAGCCAGATGCTAAGGC‑TGGGTGTTGCGCCTTGAGAATGTTca                                                                                          >  2:313513/1‑148 (MQ=255)                                                             ttCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTg                                                                                         >  6:308214/1‑149 (MQ=255)                                                                cTTTACGGCCGCTCGCGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTa                                                                                       <  5:73048/148‑1 (MQ=255)                                                                     cGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAAtt                                                                                 >  2:58537/1‑149 (MQ=255)                                                                          gCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACCGGGACCGCGCAAGGTGTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGaca                                                                            >  2:60191/1‑149 (MQ=255)                                                                              gTGGCTGGCCGAAAAGCGCGGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCCCAGATCTTGCGCAAGCCGGATGCTAAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACtt                                                                         >  6:109574/1‑148 (MQ=255)                                                                               tggctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTc                                                                       <  1:346162/149‑1 (MQ=255)                                                                                 gctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCa                                                                      <  1:333434/148‑1 (MQ=255)                                                                                 gctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCa                                                                      <  1:329158/148‑1 (MQ=255)                                                                                   tggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGGCCGCGCCAGGGGTCAGCTCTTTCGCAAGCCGGATGCTCAGGC‑TGGCGGGGGGGGCTTGAGAATGTTGTGTAGCCATTTGACACGTTGAACg                                                                   >  8:18391/1‑149 (MQ=255)                                                                                    ggcCGAAAAGCGCAGTGCTGCGGCCAACAGACTGGAGCCTGCGCCGTCCCCCAGGGGCCGCGCAAGGTGTGAGATCTTTCGCAAGCCGGATGCTCAGGC‑TGGGGGTGGCGCCGTGAGAATGTTCTGTAGCAATTGGACACTTTCAACgg                                                                  >  1:208285/1‑149 (MQ=255)                                                                                     tccGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGc                                                                 <  6:330117/148‑1 (MQ=255)                                                                                          aaaGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCAt                                                            <  5:308214/149‑1 (MQ=255)                                                                                               gcAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGAccc                                                       <  2:241176/149‑1 (MQ=255)                                                                                                cAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGAcc                                                        >  5:181515/1‑147 (MQ=255)                                                                                                 aGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCa                                                      <  6:181515/148‑1 (MQ=255)                                                                                                        gggCCAACTGACTGGAGGCTGCGCGGTCCCACAGTGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTc                                              <  1:167738/148‑1 (MQ=255)                                                                                                                   ctgGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgccgc                                    >  3:46181/1‑148 (MQ=255)                                                                                                                     agaccttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCAt                                 >  6:169036/7‑149 (MQ=255)                                                                                                                        gCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCg                              >  3:295383/1‑149 (MQ=255)                                                                                                                         cttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCgg                             >  7:126087/3‑149 (MQ=255)                                                                                                                         ccTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCgg                             >  8:54469/1‑149 (MQ=255)                                                                                                                          ttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACtt                                                                         >  2:151607/2‑104 (MQ=39)                                                                                                                          ttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACtt                                                                         <  1:151607/103‑1 (MQ=39)                                                                                                                            ccgttctCCCACAGGGACCGCTCAAGGTCTCAGCTCTTGCGCAAGCCTGCTGCTCAGGC‑TGGCTGTGGCGCCTTGAGTATGTTCTGTTGCATTTGGACACTTTCACCGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCt                           <  4:225047/142‑1 (MQ=255)                                                                                                                              gcgGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGAt                        <  5:169036/149‑1 (MQ=255)                                                                                                                                    ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCa                                  <  5:115810/133‑1 (MQ=255)                                                                                                                                    ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCa                                  >  6:115810/1‑133 (MQ=255)                                                                                                                                    ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGACCgat                    >  6:228848/1‑145 (MQ=255)                                                                                                                                    ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCACGCTGGGCAGGTACCCGCCATCCGGCTGagaacgg                   >  3:225047/1‑142 (MQ=255)                                                                                                                                     cacaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCGTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt                    >  1:276884/1‑147 (MQ=255)                                                                                                                                          ggACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGACCGATCAGccgga            <  5:228848/149‑4 (MQ=255)                                                                                                                                           gACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCtt           <  1:313513/149‑1 (MQ=255)                                                                                                                                              cgcgCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCTtctc        <  5:206849/149‑1 (MQ=255)                                                                                                                                                 gcAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCTTCTCATg     <  8:126087/149‑1 (MQ=255)                                                                                                                                                    aGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCTTCTCATGCAg  <  7:54469/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGC‑TGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCCCCTTCTCATGCAG  >  NC_002947/233699‑233994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑