Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,839,286	A→C	9.6%	R79R (CGT→CGG) ‡	ecd ←	flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,839,286	0	A	C	9.6%	193.1 / 10.2	71	R79R (CGT→CGG) ‡	ecd	flavoprotein
Reads supporting (aligned to +/- strand):  ref base A (35/28);  new base C (4/3);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCTTTCTAAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGATGCTG  >  NC_002947/2839139‑2839426                                                                                                                                                    |                                                                                                                                             gCTTTCTAAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCATGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCgaga                                                                                                                                              <  7:259172/148‑1 (MQ=255) gCTTTCTAAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGAc                                                                                                                                             >  1:133159/1‑149 (MQ=255)        aaaTAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTcgc                                                                                                                                       >  4:116003/1‑148 (MQ=255)          aTAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGCGTCTCGCGag                                                                                                                                    <  4:250079/149‑2 (MQ=255)             gTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATccc                                                                                                                                 >  5:11412/1‑149 (MQ=255)                   aCCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACgg                                                                                                                           >  7:299703/1‑149 (MQ=255)                    ccTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGc                                                                                                                          <  2:40411/149‑1 (MQ=255)                     cTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGc                                                                                                                          >  1:134837/1‑148 (MQ=255)                      tGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                        >  3:187415/1‑149 (MQ=255)                       gCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGAGTGCCAAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGCCGCTCGCGATCCCGCCCGGCGAg                                                                                                                       >  4:199612/1‑149 (MQ=255)                              gTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTc                                                                                                                >  4:183670/1‑149 (MQ=255)                               tGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTcc                                                                                                               <  4:269605/149‑1 (MQ=255)                                 ccTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCaa                                                                                                             <  5:267034/149‑1 (MQ=255)                                  cTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACCGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCAAGGCGAGCACGCTCCAAg                                                                                                            >  6:341330/1‑149 (MQ=255)                                   tGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAg                                                                                                            >  2:311206/1‑148 (MQ=255)                                   tGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGc                                                                                                           >  6:301463/1‑149 (MQ=255)                                    gAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCt                                                                                                          >  7:51835/1‑149 (MQ=255)                                       gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTg                                                                                                        >  7:312247/1‑148 (MQ=255)                                       gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGc                                                                                                       <  8:51835/149‑1 (MQ=255)                                       gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGc                                                                                                       <  1:170278/149‑1 (MQ=255)                                        ggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCa                                                                                                      <  4:187415/149‑1 (MQ=255)                                          ttCTGGCTAGTCGGCGTAGGCGTGACCACTCCACGAGCCACACCAGGAACAGCGGTGCCTGCTCGCCATGGCCCTCCTTACCGGACAGTCGGGCAAGCCTCGCGCGACGCTCGCGCTCCCCCACGGCGAGCTCGCTCCAAGATAGCacg                                                                                                    >  4:253329/1‑147 (MQ=255)                                               gCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTc                                                                                                 <  2:134837/147‑1 (MQ=255)                                               gCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGa                                                                                               <  2:133159/149‑1 (MQ=255)                                                cTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAg                                                                                              <  8:312247/149‑1 (MQ=255)                                                   gTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGaa                                                                                           <  3:107056/149‑1 (MQ=255)                                                   gTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGaa                                                                                           <  1:311206/149‑1 (MQ=255)                                                             gCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCt                                                                                  >  4:138037/1‑148 (MQ=255)                                                                   ccAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAg                                                                                          >  3:218406/1‑134 (MQ=255)                                                                     aGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGgcc                                                                         >  8:133881/1‑149 (MQ=255)                                                                      gTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGgccg                                                                        >  5:359812/1‑149 (MQ=255)                                                                         cACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGccgccg                                                                     >  4:48264/1‑149 (MQ=255)                                                                           cGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCcgccgc                                                                    >  4:16572/1‑148 (MQ=255)                                                                            gAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGa                                                                  <  3:183670/149‑1 (MQ=255)                                                                              gCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                <  3:138037/149‑1 (MQ=255)                                                                                 aGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGcc                                                             >  3:292019/1‑149 (MQ=255)                                                                                 aGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGcc                                                             <  3:16572/149‑1 (MQ=255)                                                                                       ggAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCt                                                        >  5:208079/1‑148 (MQ=255)                                                                                        gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                      <  3:116003/149‑1 (MQ=255)                                                                                        gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                      <  3:41293/149‑1 (MQ=255)                                                                                        gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                      <  5:301463/149‑1 (MQ=255)                                                                                           cAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCa                                                   >  5:250360/1‑149 (MQ=255)                                                                                           cAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCa                                                   >  6:330873/1‑149 (MQ=255)                                                                                                   ccTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCa                                           <  7:133881/149‑1 (MQ=255)                                                                                                     tGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATc                                         >  7:217033/1‑149 (MQ=255)                                                                                                      gCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTGCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATcc                                        >  6:270175/1‑149 (MQ=255)                                                                                                          gACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCcaca                                     <  6:208079/148‑1 (MQ=255)                                                                                                          gACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAg                                    >  6:230763/1‑149 (MQ=255)                                                                                                          gACATGGCGCTGGTTACCGGACAGTCGGCCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCcaca                                     <  5:330873/148‑1 (MQ=255)                                                                                                                gcgcTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAACACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGAt                              <  6:11412/149‑1 (MQ=255)                                                                                                                      tttACCGGACAGTAGGGCAAGCCTCGCGAGAAGCTCGCGATCCCGCACGGCGAGCACGTGCCAAGCTTGCAGGTTCGAGGAAGGCGAGGCTGAGTGGGCCGCCGCGATCGCGCAGCTGAGCATCTGCTCATCCACAGGAAGATCGCTg                         <  3:199612/147‑1 (MQ=255)                                                                                                                      gTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                          <  6:359812/147‑1 (MQ=255)                                                                                                                      gTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTg                         >  6:13405/1‑148 (MQ=255)                                                                                                                             gACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACg                 >  1:185346/1‑149 (MQ=255)                                                                                                                                aGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                >  3:203897/1‑99 (MQ=255)                                                                                                                                aGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                <  4:203897/99‑1 (MQ=255)                                                                                                                                aGTCGGGCAAGCCTCGAGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTAc              >  4:151154/1‑149 (MQ=255)                                                                                                                                    gggCAAGCCTCGCGAGTCGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCGATCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGAt          <  3:253329/149‑1 (MQ=255)                                                                                                                                    gggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGa           <  5:13405/148‑1 (MQ=255)                                                                                                                                     ggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCa                   >  8:206816/1‑139 (MQ=255)                                                                                                                                     ggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCa                   <  7:206816/139‑1 (MQ=255)                                                                                                                                      gCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                        >  3:99674/1‑37 (MQ=255)                                                                                                                                      gCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                        <  4:99674/37‑1 (MQ=255)                                                                                                                                        aaGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAt      >  6:75495/1‑149 (MQ=255)                                                                                                                                         gggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCg        >  7:124643/5‑146 (MQ=255)                                                                                                                                         gggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCg        <  8:124643/142‑1 (MQ=255)                                                                                                                                          ggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                          >  6:297954/4‑127 (MQ=255)                                                                                                                                          ggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                          <  5:297954/124‑1 (MQ=255)                                                                                                                                           gatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGATCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgct   >  7:186660/3‑149 (MQ=255)                                                                                                                                           ccTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgct   >  8:275014/1‑149 (MQ=255)                                                                                                                                            cTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgctg  <  5:75495/149‑1 (MQ=255)                                                                                                                                            cTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCAAAAGAAGATCGCTGTAGGCAc                  >  6:319792/1‑133 (MQ=255)                                                                                                                                                    |                                                                                                                                             GCTTTCTAAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGATGCTG  >  NC_002947/2839139‑2839426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑