Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,839,288	G→T	8.1%	R79S (CGT→AGT) ‡	ecd ←	flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,839,288	0	G	T	8.1%	221.0 / 6.9	70	R79S (CGT→AGT) ‡	ecd	flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (35/29);  new base T (3/3);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

AAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGATGCTGCAACATCTGC  >  NC_002947/2839146‑2839436                                                                                                                                               |                                                                                                                                                     aaaTAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTcgc                                                                                                                                                 >  4:116003/1‑148 (MQ=255)   aTAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGCGTCTCGCGag                                                                                                                                              <  4:250079/149‑2 (MQ=255)      gTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATccc                                                                                                                                           >  5:11412/1‑149 (MQ=255)            aCCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACgg                                                                                                                                     >  7:299703/1‑149 (MQ=255)             ccTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGc                                                                                                                                    <  2:40411/149‑1 (MQ=255)              cTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGc                                                                                                                                    >  1:134837/1‑148 (MQ=255)               tGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                                  >  3:187415/1‑149 (MQ=255)                gCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGAGTGCCAAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGCCGCTCGCGATCCCGCCCGGCGAg                                                                                                                                 >  4:199612/1‑149 (MQ=255)                       gTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTc                                                                                                                          >  4:183670/1‑149 (MQ=255)                        tGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTcc                                                                                                                         <  4:269605/149‑1 (MQ=255)                          ccTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCaa                                                                                                                       <  5:267034/149‑1 (MQ=255)                           cTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACCGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCAAGGCGAGCACGCTCCAAg                                                                                                                      >  6:341330/1‑149 (MQ=255)                            tGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAg                                                                                                                      >  2:311206/1‑148 (MQ=255)                            tGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGc                                                                                                                     >  6:301463/1‑149 (MQ=255)                             gAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCt                                                                                                                    >  7:51835/1‑149 (MQ=255)                                gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTg                                                                                                                  >  7:312247/1‑148 (MQ=255)                                gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGc                                                                                                                 <  8:51835/149‑1 (MQ=255)                                gggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGc                                                                                                                 <  1:170278/149‑1 (MQ=255)                                 ggTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCa                                                                                                                <  4:187415/149‑1 (MQ=255)                                   ttCTGGCTAGTCGGCGTAGGCGTGACCACTCCACGAGCCACACCAGGAACAGCGGTGCCTGCTCGCCATGGCCCTCCTTACCGGACAGTCGGGCAAGCCTCGCGCGACGCTCGCGCTCCCCCACGGCGAGCTCGCTCCAAGATAGCacg                                                                                                              >  4:253329/1‑147 (MQ=255)                                        gCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTc                                                                                                           <  2:134837/147‑1 (MQ=255)                                        gCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGa                                                                                                         <  2:133159/149‑1 (MQ=255)                                         cTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAg                                                                                                        <  8:312247/149‑1 (MQ=255)                                            gTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGaa                                                                                                     <  3:107056/149‑1 (MQ=255)                                            gTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGaa                                                                                                     <  1:311206/149‑1 (MQ=255)                                                      gCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCt                                                                                            >  4:138037/1‑148 (MQ=255)                                                            ccAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAg                                                                                                    >  3:218406/1‑134 (MQ=255)                                                              aGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGgcc                                                                                   >  8:133881/1‑149 (MQ=255)                                                               gTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGgccg                                                                                  >  5:359812/1‑149 (MQ=255)                                                                  cACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGccgccg                                                                               >  4:48264/1‑149 (MQ=255)                                                                    cGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCcgccgc                                                                              >  4:16572/1‑148 (MQ=255)                                                                     gAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGa                                                                            <  3:183670/149‑1 (MQ=255)                                                                       gCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                          <  3:138037/149‑1 (MQ=255)                                                                          aGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGcc                                                                       >  3:292019/1‑149 (MQ=255)                                                                          aGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGcc                                                                       <  3:16572/149‑1 (MQ=255)                                                                                ggAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCt                                                                  >  5:208079/1‑148 (MQ=255)                                                                                 gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                                <  3:116003/149‑1 (MQ=255)                                                                                 gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                                <  3:41293/149‑1 (MQ=255)                                                                                 gAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCa                                                                <  5:301463/149‑1 (MQ=255)                                                                                    cAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCa                                                             >  5:250360/1‑149 (MQ=255)                                                                                    cAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCa                                                             >  6:330873/1‑149 (MQ=255)                                                                                            ccTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCa                                                     <  7:133881/149‑1 (MQ=255)                                                                                              tGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATc                                                   >  7:217033/1‑149 (MQ=255)                                                                                               gCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTGCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATcc                                                  >  6:270175/1‑149 (MQ=255)                                                                                                   gACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCcaca                                               <  6:208079/148‑1 (MQ=255)                                                                                                   gACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAg                                              >  6:230763/1‑149 (MQ=255)                                                                                                   gACATGGCGCTGGTTACCGGACAGTCGGCCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCcaca                                               <  5:330873/148‑1 (MQ=255)                                                                                                         gcgcTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAACACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGAt                                        <  6:11412/149‑1 (MQ=255)                                                                                                               tttACCGGACAGTAGGGCAAGCCTCGCGAGAAGCTCGCGATCCCGCACGGCGAGCACGTGCCAAGCTTGCAGGTTCGAGGAAGGCGAGGCTGAGTGGGCCGCCGCGATCGCGCAGCTGAGCATCTGCTCATCCACAGGAAGATCGCTg                                   <  3:199612/147‑1 (MQ=255)                                                                                                               gTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                                    <  6:359812/147‑1 (MQ=255)                                                                                                               gTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTg                                   >  6:13405/1‑148 (MQ=255)                                                                                                                      gACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACg                           >  1:185346/1‑149 (MQ=255)                                                                                                                         aGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                          <  4:203897/99‑1 (MQ=255)                                                                                                                         aGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATc                                                                          >  3:203897/1‑99 (MQ=255)                                                                                                                         aGTCGGGCAAGCCTCGAGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTAc                        >  4:151154/1‑149 (MQ=255)                                                                                                                             gggCAAGCCTCGCGAGTCGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCGATCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGAt                    <  3:253329/149‑1 (MQ=255)                                                                                                                             gggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGa                     <  5:13405/148‑1 (MQ=255)                                                                                                                              ggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCa                             >  8:206816/1‑139 (MQ=255)                                                                                                                              ggCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCa                             <  7:206816/139‑1 (MQ=255)                                                                                                                               gCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                                  >  3:99674/1‑37 (MQ=255)                                                                                                                               gCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGa                                                                                                                                  <  4:99674/37‑1 (MQ=255)                                                                                                                                 aaGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAt                >  6:75495/1‑149 (MQ=255)                                                                                                                                  gggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCg                  >  7:124643/5‑146 (MQ=255)                                                                                                                                  gggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCg                  <  8:124643/142‑1 (MQ=255)                                                                                                                                   ggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                                    <  5:297954/124‑1 (MQ=255)                                                                                                                                   ggatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCt                                    >  6:297954/4‑127 (MQ=255)                                                                                                                                    gatCGCGAGCGTCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGATCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgct             >  7:186660/3‑149 (MQ=255)                                                                                                                                    ccTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgct             >  8:275014/1‑149 (MQ=255)                                                                                                                                     cTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGAtgctg            <  5:75495/149‑1 (MQ=255)                                                                                                                                     cTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCAAAAGAAGATCGCTGTAGGCAc                            >  6:319792/1‑133 (MQ=255)                                                                                                                                               gCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGATGCTGCAACATCTgc  >  6:177070/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     AAATAGTCGATACCTGCAGTCGGTGCCTGAAGGGTTCTGGCTAGTCGGCGTAGGCGTGACCAGTCCACGAGCCAGACCAGGAACAGCGGTGCCTGCTCGACATGGCGCTGGTTACCGGACAGTCGGGCAAGCCTCGCGAGACGCTCGCGATCCCGCACGGCGAGCACGCTCCAAGCTTGCAGGTTCGAGGAAGTCGAGGCTGACTGGGCCGCCGCGATCGCCCAGCTCAGCATCTGCTCATCCACAGGAAGATCGCTGTAGGCACGTACTGATCGATGCTGCAACATCTGC  >  NC_002947/2839146‑2839436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑