Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,884,556 C→T 7.2% G7G (GGC→GGT)  PP_2540 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,884,5560CT 7.2% 160.5 / 2.4 55G7G (GGC→GGT) PP_2540short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

AGCGGCGGTTATCGGTGGATCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCG  >  NC_002947/2884415‑2884703
                                                                                                                                              |                                                                                                                                                   
aGCGGCGGTTATCGGTGGATCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTgg                                                                                                                                               <  5:132841/148‑1 (MQ=255)
        ttATCGGTGGATCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTc                                                                                                                                      >  7:215782/1‑149 (MQ=255)
                  aTCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGACTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATATAGGCATCACCGGCCGCTGGGCCATCGTCTGcgccgc                                                                                                                              >  5:370387/1‑147 (MQ=255)
                   tCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAGTCAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGgcca                                                                                                                            <  1:96395/149‑1 (MQ=255)
                    cAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGgccagc                                                                                                                          >  6:274927/1‑149 (MQ=255)
                     aGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGgccagc                                                                                                                          >  2:366121/1‑148 (MQ=255)
                       ttCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGgccagcc                                                                                                                         >  2:48233/1‑147 (MQ=255)
                       ttCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGgccagcc                                                                                                                         >  7:130119/1‑147 (MQ=255)
                         cTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAg                                                                                                                      <  8:215782/148‑1 (MQ=255)
                         cTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAg                                                                                                                      <  6:168035/148‑1 (MQ=255)
                              cATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCtt                                                                                                                 <  5:220006/148‑1 (MQ=255)
                               aTTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTgg                                                                                                               >  4:318051/1‑149 (MQ=255)
                               aTTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTg                                                                                                                <  2:1032/148‑1 (MQ=255)
                                           ccTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGAAAAGGCTgcgc                                                                                                   >  7:252939/1‑149 (MQ=255)
                                               tCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGaa                                                                                               <  1:366121/149‑1 (MQ=255)
                                                        ctAGATTGCCCCGGCAGCGCCTGTCAGTCAGCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCCAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTggg                                                                                       >  5:54187/1‑148 (MQ=255)
                                                        ctAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTggg                                                                                       >  3:47389/1‑148 (MQ=255)
                                                            aTTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAaag                                                                                  <  5:196678/149‑1 (MQ=255)
                                                               gCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCt                                                                                                                  >  8:222758/1‑114 (MQ=255)
                                                               gCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCt                                                                                                                  <  7:222758/114‑1 (MQ=255)
                                                                ccccGGCAGCGCCTGTCAGGCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAaagaag                                                                               <  5:274927/148‑1 (MQ=255)
                                                                   cGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAaagaag                                                                               >  3:93487/1‑145 (MQ=255)
                                                                   cGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAaagaag                                                                               <  4:93487/145‑1 (MQ=255)
                                                                          gcCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTg                                                                    >  5:352657/1‑149 (MQ=255)
                                                                            cTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGTCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGt                                                                  >  1:348682/1‑149 (MQ=255)
                                                                            cTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGTCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAAAAGGCGTCAACCTGGt                                                                  >  3:328656/1‑149 (MQ=255)
                                                                               tcagtcaCCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGtcat                                                               <  1:48233/149‑1 (MQ=255)
                                                                                 agtcaCCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGAAGAAAAGGCCGCTGGGCCATCGTCTGAGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGtcatc                                                              >  5:257354/1‑148 (MQ=255)
                                                                                      ccccTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGcc                                                        >  4:167882/1‑149 (MQ=255)
                                                                                               cccTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTAccc                                                 >  4:347181/1‑147 (MQ=255)
                                                                                                ccTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAg                                              >  4:261034/1‑149 (MQ=255)
                                                                                                ccTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAg                                              <  3:318051/149‑1 (MQ=255)
                                                                                                 cTGATGGCAGCCAGGAG‑CACACCACTGGCTCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGc                                             <  8:252939/149‑1 (MQ=255)
                                                                                                        gaTCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGTGCCATCGTCTGCGCGGCCAGCCAAGTCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTg                                      <  6:370387/148‑1 (MQ=255)
                                                                                                        cATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCActct                                       <  8:130119/148‑1 (MQ=255)
                                                                                                         aTCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTg                                      >  5:266703/1‑148 (MQ=255)
                                                                                                         aTCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTg                                      >  5:45883/1‑148 (MQ=255)
                                                                                                         aTCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTg                                      >  2:181518/1‑148 (MQ=255)
                                                                                                          tCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGa                                    <  6:266703/149‑1 (MQ=255)
                                                                                                            aaGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGTCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAg                                   <  1:92503/148‑1 (MQ=255)
                                                                                                               gAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTAc                                                   <  5:320486/129‑1 (MQ=255)
                                                                                                               gAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTAc                                                   >  6:320486/1‑129 (MQ=255)
                                                                                                                     aaCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAgc                                                                                             <  3:340908/82‑1 (MQ=255)
                                                                                                                     aaCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAgc                                                                                             >  4:340908/1‑82 (MQ=255)
                                                                                                                     aaCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGcc                          <  6:352657/149‑1 (MQ=255)
                                                                                                                     aaCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGc                           >  7:123262/1‑148 (MQ=255)
                                                                                                                      aCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCa                                 <  2:243368/141‑1 (MQ=255)
                                                                                                                      aCCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCa                                 >  1:243368/1‑141 (MQ=255)
                                                                                                                       ccAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAg                        <  7:167982/149‑1 (MQ=255)
                                                                                                                             gATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGtt                  <  3:167882/149‑1 (MQ=255)
                                                                                                                                    gCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAgg           >  2:138022/1‑149 (MQ=255)
                                                                                                                                     cATCACCGGTCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAggg          <  2:348682/149‑1 (MQ=255)
                                                                                                                                     cATCACCGGTCGCTGGGCCATCGTCAGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAACAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAggg          <  4:328656/149‑1 (MQ=255)
                                                                                                                                          ccGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGc     >  1:61619/1‑149 (MQ=255)
                                                                                                                                             gCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCg  >  2:354128/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
AGCGGCGGTTATCGGTGGATCAGTTCTTTTCATTATCGGTGGCCCTGTCCCGTCCTCTAGATTGCCCCGGCAGCGCCTGTCAGTCACCCCTGTCACCCTGATTGCATCAAGGAG‑CAAACCAATGGATCTAGGCATCACCGGCCGCTGGGCCATCGTCTGCGCGGCCAGCCAAGGCCTTGGCAAAGGCTGCGCCGAAGCGCTGGGCAAAGAAGGCGTCAACCTGGTCATCAATGCCCGTACCCAAGCCACTCTGGAGCAGACCGCCAGCGAGTTGCGCAGGGCCTGCCCG  >  NC_002947/2884415‑2884703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑