Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,921,460 G→A 100% G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA100.0% 160.0 / NA 48G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/19);  total (29/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT  >  NC_002947/2921316‑2921604
                                                                                                                                                |                                                                                                                                                  
acGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGGGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggg                                                                                                                                                <  8:135079/149‑1 (MQ=255)
      cTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCg                                                                                                                                           <  6:175275/148‑1 (MQ=255)
       cgggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                          >  1:155298/2‑148 (MQ=255)
          cggccgCCGGTAACGCCCACCTGCACCGGGGCGCTGGCGATGACCGCCTGGATGGCGGAAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAAc                                                                                                                                      <  6:64612/144‑1 (MQ=255)
           cgccgCCGGGAACGACACCCTGACCGGGGGCGCTGGCGAGGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACg                                                                                                                                     <  4:359200/145‑1 (MQ=255)
            gAGGCCGGAAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATCACACCCTGCAGGCCGGCGATGCTAACGACACATTGTTCGGCGACGAGGGTACCGAAACCATGATCAGGGGGGGCGGCacagc                                                                                                                                    >  6:223414/1‑145 (MQ=255)
                 cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                          >  1:64573/1‑138 (MQ=255)
                 cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                          <  2:64573/138‑1 (MQ=255)
                 cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                                >  5:289906/1‑148 (MQ=255)
                     aaCGTCATCCTGAACGCGGGCGCTGGCGTTGACAGCCTGGATGGCGGCAAGGGCAATGACTCCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCaa                                                                                                                           <  5:223414/149‑1 (MQ=255)
                           aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                     <  4:151222/149‑1 (MQ=255)
                              cTGCACGGCCGCGCTGGGGATGACAGCCTGGAGGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGCGCGG‑‑c                                                                                                                <  7:14714/149‑1 (MQ=255)
                              cTGAACGGGGGCGTTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCAGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                   >  2:107113/1‑148 (MQ=255)
                                    gggggCGCTGGCGATGACAGCCTGGATGGCGACAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTaa                                                                                                          >  8:55200/1‑149 (MQ=255)
                                            tGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGCCGATCGTAAAGACACATTGTTCGGCGACGTGGGTAGCGCCACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACgg                                                                                                  >  7:194819/1‑149 (MQ=255)
                                              gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACgg                                                                                                  <  6:289906/147‑1 (MQ=255)
                                                gATGAGAGCCTGGATGGCGGCAACGGGAATGTCACCGTGCAGGGCGGCGATGGTAACGACACTTTGTTCGGCGACTTTGGTACCGACACCGTGATCAGTGGGGCCGGCAACTACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACggtggt                                                                                              <  8:194819/149‑1 (MQ=255)
                                                gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACggtggt                                                                                              >  1:142951/1‑149 (MQ=255)
                                                      aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGgtgggtgg                                                                                        <  2:155298/149‑1 (MQ=255)
                                                              tGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGt                                                                                 >  3:123831/1‑148 (MQ=255)
                                                                   gcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa                                                                            >  8:117267/1‑148 (MQ=255)
                                                                     aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGTTACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                         <  1:107113/149‑1 (MQ=255)
                                                                        ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                      >  5:28846/1‑149 (MQ=255)
                                                                                  cccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                            >  1:139280/1‑149 (MQ=255)
                                                                                              gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                                <  7:117267/149‑1 (MQ=255)
                                                                                                     tAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                          >  7:254130/1‑148 (MQ=255)
                                                                                                          acacATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTc                                    >  4:367980/1‑149 (MQ=255)
                                                                                                          acacATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTc                                    >  4:337900/1‑149 (MQ=255)
                                                                                                            acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                  >  3:350686/1‑149 (MQ=255)
                                                                                                              aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGGACAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                  >  1:115238/1‑149 (MQ=255)
                                                                                                              aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                                >  8:4033/1‑149 (MQ=255)
                                                                                                              aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCAGGCACAGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGCCACCATGATCGCCACCGATGGAAACGATGTGTTCAAGTTCGCTg                                >  5:262297/1‑149 (MQ=255)
                                                                                                                  ttCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                             <  4:350686/148‑1 (MQ=255)
                                                                                                                       cGACGTGGGTACCGACACCCTTATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATTATCGCCACCGATGGAAACTTTGTGTTCCAGTTCGCTGCAGGCTTTg                       <  7:55200/149‑1 (MQ=255)
                                                                                                                       cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                       <  2:139280/149‑1 (MQ=255)
                                                                                                                               gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                          >  6:88217/1‑122 (MQ=255)
                                                                                                                               gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                          <  5:88217/122‑1 (MQ=255)
                                                                                                                                   cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa           >  6:263297/1‑149 (MQ=255)
                                                                                                                                    gACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat          >  5:129456/1‑149 (MQ=255)
                                                                                                                                     acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat          >  2:50520/1‑148 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACgg                                                                                                  >  3:302047/1‑56 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACgg                                                                                                  <  4:302047/56‑1 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTggtgggtg                                                                                         >  5:140849/1‑65 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTggtgggtg                                                                                         <  6:140849/65‑1 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc      >  5:240349/1‑148 (MQ=255)
                                                                                                                                         ccTGAGCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCACCGACCTGAtcatca     >  6:264681/1‑149 (MQ=255)
                                                                                                                                          cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCaa    <  4:123831/149‑1 (MQ=255)
                                                                                                                                          cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGACGGTAACGACAACAAGAACGCCAACAATGGAAACCATCACTACCAGATCGATGCAGGATTTAGAACCGACCTGaacatgca    >  4:229787/1‑146 (MQ=255)
                                                                                                                                            gATCAGGGGGGCCGGCAACGACGTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAAGACACCATGATCGCCACCGATGGAAACGATTTGTTCCCGTTCGCTGCAGGCTTTGGCAACGCCCTGATCATCAAtt  >  7:231658/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGG‑‑CAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT  >  NC_002947/2921316‑2921604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑