Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,006,474 A→C 6.2% K1019Q (AAG→CAG)  PP_2627 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,006,4740AC 6.2% 255.8 / 5.7 80K1019Q (AAG→CAG) PP_2627membrane protein
Reads supporting (aligned to +/- strand):  ref base A (38/37);  new base C (3/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGC  >  NC_002947/3006326‑3006621
                                                                                                                                                    |                                                                                                                                                   
cGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGa                                                                                                                                                     <  3:218584/149‑1 (MQ=255)
  tgtACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAg                                                                                                                                                   <  5:236598/149‑1 (MQ=255)
      cAGTTTTTTCCAGCAACGCCTAGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                                >  7:49984/1‑148 (MQ=255)
       aGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACaa                                                                                                                                              >  5:4923/1‑149 (MQ=255)
         ttttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAcc                                                                                                                                            >  4:92191/1‑149 (MQ=255)
         ttttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATAATCGGTATCTCAAGGTTTTCCTGAAGGACAAcc                                                                                                                                            >  8:143709/1‑149 (MQ=255)
            tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCtt                                                                                                                                          >  6:327825/1‑148 (MQ=255)
                 aGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCTTGAga                                                                                                                                      <  1:201999/147‑2 (MQ=255)
                 aGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCTTGAga                                                                                                                                      >  2:201999/1‑146 (MQ=255)
                  gCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCt                                                                                                                                   >  2:72824/1‑149 (MQ=255)
                    aaCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                 <  3:104480/149‑1 (MQ=255)
                    aaCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                 <  3:92191/149‑1 (MQ=255)
                    aaCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                 <  2:178737/149‑1 (MQ=255)
                     aCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                 >  1:155514/1‑148 (MQ=255)
                            gCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTcc                                                                                                                           <  7:326045/147‑1 (MQ=255)
                                  cGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCa                                                                                                                   >  1:197799/1‑149 (MQ=255)
                                   gCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATAATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCAc                                                                                                                  <  7:143709/149‑1 (MQ=255)
                                    gctacccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACg                                                                                                                 >  6:303345/5‑149 (MQ=255)
                                    cTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACg                                                                                                                 <  6:4923/149‑1 (MQ=255)
                                       ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACggg                                                                                                               <  3:264841/148‑1 (MQ=255)
                                       ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACgg                                                                                                                <  2:331640/147‑1 (MQ=255)
                                          cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAAt                                                                                                           <  7:279966/149‑1 (MQ=255)
                                               tggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCt                                                                                                       <  5:134538/148‑1 (MQ=255)
                                               tggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCt                                                                                                       >  3:144424/1‑148 (MQ=255)
                                                   gAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTc                                                                                                  <  8:184056/149‑1 (MQ=255)
                                                      ggCACCCGATGCTTGTTTGGATGACATCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTg                                                                                               >  8:10405/1‑149 (MQ=255)
                                                      ggCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTg                                                                                               >  1:259170/1‑149 (MQ=255)
                                                       gCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGc                                                                                              <  2:197799/149‑1 (MQ=255)
                                                        cACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCGTGAAGGACAACGTTCGGGCCTTGCGGTCCGCTCAGCCCGGGCATGCCGGGATTCGTGcc                                                                                             >  6:30448/1‑149 (MQ=255)
                                                         aCCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGcc                                                                                             >  6:304316/1‑148 (MQ=255)
                                                           ccGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGAc                                                                                          >  2:132241/1‑149 (MQ=255)
                                                              aTGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGt                                                                                        >  8:305425/1‑148 (MQ=255)
                                                                  ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATc                                                                                    >  5:260416/1‑148 (MQ=255)
                                                                     tttGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                 >  6:2521/1‑148 (MQ=255)
                                                                       ttgATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGagca                                                                               <  4:273371/146‑1 (MQ=255)
                                                                       tGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCTGTATCTCATGGTTTTCCTGAAGGTCTACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGagca                                                                               <  7:106384/148‑1 (MQ=255)
                                                                       tGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                 <  7:305425/146‑1 (MQ=255)
                                                                       tGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                 <  1:72824/146‑1 (MQ=255)
                                                                         gATGACTTCGAGGCGTTCTTCGGGCAGAAAGGGAGCCTGCAGCTGTTTAATGATCGGTATCTCAAGGTTTTCCAGAAGGAGAACCTAGAGGCCTTGCAGTACGCTATCAACTGGCAATACATGATTCGTGCCGCCGTGATCGag                                                                                 >  7:308644/1‑144 (MQ=255)
                                                                            gACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTc                                                                           >  8:239556/1‑147 (MQ=255)
                                                                              cTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGc                                                                       <  8:49984/149‑1 (MQ=255)
                                                                                   aGGCGTTATTCGGGCCTAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCg                                                                  <  6:260416/149‑1 (MQ=255)
                                                                                       gTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                              <  7:216432/149‑1 (MQ=255)
                                                                                           ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGc                                                                   >  5:374604/1‑140 (MQ=255)
                                                                                           ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGc                                                                   <  6:374604/140‑1 (MQ=255)
                                                                                           ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCAt                                                          >  7:260404/1‑149 (MQ=255)
                                                                                             cGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCAt                                                          <  8:260404/147‑1 (MQ=255)
                                                                                             cGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATcc                                                        >  5:315131/1‑149 (MQ=255)
                                                                                              gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATcc                                                        <  5:2521/148‑1 (MQ=255)
                                                                                               ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                      <  4:144424/149‑1 (MQ=255)
                                                                                                  cGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGa                                                    >  6:359919/1‑148 (MQ=255)
                                                                                                   gAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGaaa                                                  <  5:30448/149‑1 (MQ=255)
                                                                                                          cgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                           <  2:259170/149‑1 (MQ=255)
                                                                                                          cgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                           <  2:155514/149‑1 (MQ=255)
                                                                                                          cgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                           <  5:327825/149‑1 (MQ=255)
                                                                                                                    tgTTTACTGATCGGTATCTCCAGGTCTTAGTGAATGACAACATTGAGGACGTGAAGTCCGCTCAGCAAGGGCAATCCCTTATTCTTGCCGACGTGATCGAGCAGCTCGATCTTGCGGATCGCATCCGCGAAACCTTCTTCGATCAGCGt                                 <  8:308644/148‑1 (MQ=255)
                                                                                                                      tttAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTgg                               >  7:261530/1‑149 (MQ=255)
                                                                                                                        tAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGc                              >  5:124124/1‑148 (MQ=255)
                                                                                                                        tAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGc                              >  2:153692/1‑148 (MQ=255)
                                                                                                                            gATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGAc                                                                                          >  3:194961/1‑84 (MQ=255)
                                                                                                                            gATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGAc                                                                                          <  4:194961/84‑1 (MQ=255)
                                                                                                                             aTCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCt                         <  6:315131/148‑1 (MQ=255)
                                                                                                                              tCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTaa                       <  5:303345/149‑1 (MQ=255)
                                                                                                                                ggTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgt                     <  5:304316/149‑1 (MQ=255)
                                                                                                                                ggTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgt                     <  8:296222/149‑1 (MQ=255)
                                                                                                                                 gTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgtg                    >  1:338760/1‑149 (MQ=255)
                                                                                                                                 gTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgtg                    >  3:356710/1‑149 (MQ=255)
                                                                                                                                 gTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgtg                    >  6:156392/1‑149 (MQ=255)
                                                                                                                                    tctcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCGGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGtgtg                  <  5:359919/148‑1 (MQ=255)
                                                                                                                                    cctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTTGCAAGCTAAGtgtg                  >  1:311268/2‑148 (MQ=255)
                                                                                                                                     ctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGaaa                                                  >  2:6455/1‑115 (MQ=39)
                                                                                                                                     ctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGaaa                                                  <  1:6455/115‑1 (MQ=39)
                                                                                                                                      tcAAGGTTTTGCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGGAAGCTAAGTGTGCAg               <  7:10405/149‑1 (MQ=255)
                                                                                                                                      tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg               >  7:324948/1‑149 (MQ=255)
                                                                                                                                      tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg               >  2:50496/1‑149 (MQ=255)
                                                                                                                                        aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt             >  8:105415/1‑149 (MQ=255)
                                                                                                                                        aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTAATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt             >  4:19278/1‑149 (MQ=255)
                                                                                                                                          ggTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt             >  5:28165/1‑147 (MQ=255)
                                                                                                                                          ggTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCa           <  1:132241/149‑1 (MQ=255)
                                                                                                                                                  tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGATCAGCATCGAg   >  6:66271/1‑149 (MQ=255)
                                                                                                                                                   gAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGc  >  4:195198/1‑149 (MQ=255)
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CGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGC  >  NC_002947/3006326‑3006621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑