Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,018,489 A→G 10.0% I43V (ATC→GTC) ‡ bcsZ → cellulose synthase endo‑1,4‑D‑glucanase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,018,4890AG10.0% 103.9 / 3.1 40I43V (ATC→GTC) ‡bcsZcellulose synthase endo‑1,4‑D‑glucanase subunit
Reads supporting (aligned to +/- strand):  ref base A (17/19);  new base G (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GTGATGATGCGCCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGG  >  NC_002947/3018357‑3018635
                                                                                                                                    |                                                                                                                                                  
gTGATGATGCGCCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGa                                                                                                                                    <  8:97751/149‑1 (MQ=255)
   atgatgCGCCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAgc                                                                                                                                  <  1:69978/148‑1 (MQ=255)
    tgatgCGCCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAgcgc                                                                                                                                >  4:118704/1‑149 (MQ=255)
         cgcCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCGGTTTTCGCCAATGACGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCTGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGAt                                                                                                                           <  5:34541/149‑1 (MQ=255)
           ccAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATca                                                                                                                         >  7:6880/1‑149 (MQ=255)
            cAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATcac                                                                                                                        <  5:276555/149‑1 (MQ=255)
              gATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATcacca                                                                                                                      >  4:3810/1‑149 (MQ=255)
                   tGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGGCTGATCACCGCCTCg                                                                                                                 >  4:286618/1‑149 (MQ=255)
                    ggTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAg                                                                                                                                          <  7:285852/123‑1 (MQ=255)
                    ggTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAg                                                                                                                                          >  8:285852/1‑123 (MQ=255)
                      tAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGaa                                                                                                              <  7:168997/149‑1 (MQ=255)
                                   cTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGt                                                                                                     <  3:336/145‑1 (MQ=255)
                                   cTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGt                                                                                                     >  4:336/1‑145 (MQ=255)
                                    ttAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGaa                                                                                                              >  6:350021/1‑135 (MQ=255)
                                    ttAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGaa                                                                                                              <  5:350021/135‑1 (MQ=255)
                                             cccGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGttctt                                                                                       >  2:269190/1‑149 (MQ=255)
                                             cccGTTTTCGCCAATGACGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGttctt                                                                                       >  6:88077/1‑149 (MQ=255)
                                              ccGTTTTCGCCTATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTGAAGGGCGAGCTGGTGAGGGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGttcttc                                                                                      <  2:77075/149‑1 (MQ=255)
                                              ccGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGttcttc                                                                                      >  3:42043/1‑149 (MQ=255)
                                                        cAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTggt                                                                              <  8:96664/147‑1 (MQ=255)
                                                             ccGGCTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTaa                                                                        <  8:6880/148‑1 (MQ=255)
                                                                     gTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCgg                                                                 <  5:224410/147‑1 (MQ=255)
                                                                     gTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCgg                                                                 >  6:224410/1‑147 (MQ=255)
                                                                       ttGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCGATGACCCgccc                                                                                                                                         >  7:149084/1‑70 (MQ=255)
                                                                       ttGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCGATGACCCgccc                                                                                                                                         <  2:177315/73‑4 (MQ=255)
                                                                       ttGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCGATGACCCgccc                                                                                                                                         <  8:149084/73‑4 (MQ=255)
                                                                       ttGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCGATGACCCgccc                                                                                                                                         >  1:177315/1‑70 (MQ=255)
                                                                        tGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCaaa                                                             <  3:118704/148‑1 (MQ=255)
                                                                         gCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAAc                                                            <  3:3810/148‑1 (MQ=255)
                                                                         gCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAAc                                                            <  7:272360/148‑1 (MQ=255)
                                                                          cccATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCt                                                          >  7:226247/1‑149 (MQ=255)
                                                                                       tgGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTtgct                                             >  5:94163/1‑149 (MQ=255)
                                                                                               gTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGa                                     >  5:144960/1‑149 (MQ=255)
                                                                                                ttCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGAGTGCAGTTACTCGCTCTTATTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGAc                                    >  1:304506/1‑149 (MQ=255)
                                                                                                    aGGCCGAGGTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGc                                <  6:94163/149‑1 (MQ=255)
                                                                                                      gCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCtt                                                         >  1:59700/1‑122 (MQ=255)
                                                                                                      gCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCtt                                                         <  2:59700/122‑1 (MQ=255)
                                                                                                      gCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGcaa                              <  6:144960/149‑1 (MQ=255)
                                                                                                               gTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGc                     <  1:269190/149‑1 (MQ=255)
                                                                                                                                  tCATCGACCCGAGGGACGCGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCCAACCTTCGCACAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCgg  >  6:292038/1‑149 (MQ=255)
                                                                                                                                    |                                                                                                                                                  
GTGATGATGCGCCAGATGCTGGTAGGGCTGGTCGCCTTAGGCCTGCCCGTTTTCGCCAATGCCGGGTCGGTTTGCCCATGGCCTGCCTGGGAGCGGTTCAAGGCCGAGCTGGTGAGTGTGGATGGGCGGGTCATCGACCCGAGTGACGAGCGCCTGATCACCACCTCGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGG  >  NC_002947/3018357‑3018635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑