Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,050,733 G→T 11.7% A189S (GCA→TCA) ‡ PP_2663 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,050,7330GT11.7% 93.9 / 3.1 34A189S (GCA→TCA) ‡PP_2663hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (14/16);  new base T (2/2);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GATGGCTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGA  >  NC_002947/3050589‑3050881
                                                                                                                                                |                                                                                                                                                    
gATGGCTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGgcg                                                                                                                                                      <  7:154574/145‑1 (MQ=255)
gATGGCTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGgcg                                                                                                                                                      >  8:154574/1‑145 (MQ=255)
     cTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtgg                                                                                                                                             >  5:346330/1‑149 (MQ=255)
      ttGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtggt                                                                                                                                            >  7:192483/1‑149 (MQ=255)
          ccAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCCGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGGGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCCGCCgtggtggtg                                                                                                                                        >  4:233585/1‑149 (MQ=255)
          ccAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtggtggtg                                                                                                                                        >  7:313692/1‑149 (MQ=255)
           cAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACCACGGCTGCGCCgttgtggaat                                                                                                                                       <  7:183171/149‑4 (MQ=255)
                gcgAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGTGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTc                                                                                                                                  <  7:88185/149‑1 (MQ=255)
                gcgAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTc                                                                                                                                  <  1:322197/149‑1 (MQ=255)
                  gaggagATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCaa                                                                                                                                >  6:163205/1‑149 (MQ=255)
                     gagaTGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAacac                                                                                                                             <  2:222400/149‑1 (MQ=255)
                        aTGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGATCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCt                                                                                                                           <  4:275096/148‑1 (MQ=255)
                              cTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGa                                                                                                                    <  6:346330/149‑1 (MQ=255)
                               ttGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                   >  6:363237/1‑149 (MQ=255)
                                  ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCTCCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                <  2:320817/149‑1 (MQ=255)
                                  ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                   <  2:223657/146‑1 (MQ=255)
                                  ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                   >  1:223657/1‑146 (MQ=255)
                                  ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                <  3:227438/149‑1 (MQ=255)
                                  ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGAGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                <  5:364643/149‑1 (MQ=255)
                                     cACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGaa                                                                                                             <  5:363237/149‑1 (MQ=255)
                                       cTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAgt                                                                                                           >  8:189962/1‑149 (MQ=255)
                                            cgcAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTca                                                                                                      <  5:144546/149‑1 (MQ=255)
                                            cgcAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTca                                                                                                      >  6:144546/1‑149 (MQ=255)
                                                gcaCTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTcaccac                                                                                                  <  7:189962/149‑1 (MQ=255)
                                                                           ggcTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGaaaa                                                                       <  8:313692/149‑1 (MQ=255)
                                                                                 gCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCtggt                                                                 <  1:229365/149‑1 (MQ=255)
                                                                                          gacAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCgg                                                        >  2:303236/1‑149 (MQ=255)
                                                                                           acAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGt                                                       >  4:183419/1‑149 (MQ=255)
                                                                                                    tGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGAcagc                                              >  4:280619/1‑149 (MQ=255)
                                                                                                         cGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGAcagcgcc                                           >  8:199506/1‑147 (MQ=255)
                                                                                                                aTGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCgg                                  <  5:163205/149‑1 (MQ=255)
                                                                                                                           ggcggTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACt                       <  8:192483/149‑1 (MQ=255)
                                                                                                                                ccagcaccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGaa                         >  2:74440/7‑142 (MQ=255)
                                                                                                                                ccagcaccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGaa                         <  1:74440/136‑1 (MQ=255)
                                                                                                                                      ccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGc            >  7:222058/1‑149 (MQ=255)
                                                                                                                                      ccACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGc            >  8:350709/1‑149 (MQ=255)
                                                                                                                                                gcAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGa  <  3:183419/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GATGGCTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGA  >  NC_002947/3050589‑3050881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑