Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,050,734 C→G 11.8% A189G (GCA→GGA) ‡ PP_2663 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,050,7340CG11.8% 91.7 / 4.9 34A189G (GCA→GGA) ‡PP_2663hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (14/16);  new base G (2/2);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

CTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGAG  >  NC_002947/3050594‑3050882
                                                                                                                                            |                                                                                                                                                    
cTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtgg                                                                                                                                              >  5:346330/1‑149 (MQ=255)
 ttGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtggt                                                                                                                                             >  7:192483/1‑149 (MQ=255)
     ccAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCCGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGGGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCCGCCgtggtggtg                                                                                                                                         >  4:233585/1‑149 (MQ=255)
     ccAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCgtggtggtg                                                                                                                                         >  7:313692/1‑149 (MQ=255)
      cAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACCACGGCTGCGCCgttgtggaat                                                                                                                                        <  7:183171/149‑4 (MQ=255)
           gcgAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGTGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTc                                                                                                                                   <  7:88185/149‑1 (MQ=255)
           gcgAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTc                                                                                                                                   <  1:322197/149‑1 (MQ=255)
             gaggagATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCaa                                                                                                                                 >  6:163205/1‑149 (MQ=255)
                gagaTGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAacac                                                                                                                              <  2:222400/149‑1 (MQ=255)
                   aTGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGATCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCt                                                                                                                            <  4:275096/148‑1 (MQ=255)
                         cTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGa                                                                                                                     <  6:346330/149‑1 (MQ=255)
                          ttGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                    >  6:363237/1‑149 (MQ=255)
                             ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCTCCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                 <  2:320817/149‑1 (MQ=255)
                             ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                    >  1:223657/1‑146 (MQ=255)
                             ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGAc                                                                                                                    <  2:223657/146‑1 (MQ=255)
                             ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                 <  3:227438/149‑1 (MQ=255)
                             ccGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGAGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTc                                                                                                                 <  5:364643/149‑1 (MQ=255)
                                cACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGaa                                                                                                              <  5:363237/149‑1 (MQ=255)
                                  cTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAgt                                                                                                            >  8:189962/1‑149 (MQ=255)
                                       cgcAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTca                                                                                                       <  5:144546/149‑1 (MQ=255)
                                       cgcAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTca                                                                                                       >  6:144546/1‑149 (MQ=255)
                                           gcaCTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTcaccac                                                                                                   <  7:189962/149‑1 (MQ=255)
                                                                      ggcTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGaaaa                                                                        <  8:313692/149‑1 (MQ=255)
                                                                            gCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCtggt                                                                  <  1:229365/149‑1 (MQ=255)
                                                                                     gacAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCgg                                                         >  2:303236/1‑149 (MQ=255)
                                                                                      acAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGt                                                        >  4:183419/1‑149 (MQ=255)
                                                                                               tGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGAcagc                                               >  4:280619/1‑149 (MQ=255)
                                                                                                    cGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGAcagcgcc                                            >  8:199506/1‑147 (MQ=255)
                                                                                                           aTGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCgg                                   <  5:163205/149‑1 (MQ=255)
                                                                                                                      ggcggTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACt                        <  8:192483/149‑1 (MQ=255)
                                                                                                                           ccagcaccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGaa                          >  2:74440/7‑142 (MQ=255)
                                                                                                                           ccagcaccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGaa                          <  1:74440/136‑1 (MQ=255)
                                                                                                                                 ccaccaCGGCTGCGCCGTTGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGc             >  7:222058/1‑149 (MQ=255)
                                                                                                                                 ccACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGc             >  8:350709/1‑149 (MQ=255)
                                                                                                                                           gcAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGa   <  3:183419/149‑1 (MQ=255)
                                                                                                                                            cAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGAg  <  3:280619/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CTTGTCCAGCCGCGAGGAGATGGTGCTTGCCGCACTCAGCGCAGCACTGGGGGATATCCCGCACTTCGGCGGCTCGGCCGGTGACGACAATTACCTGAACCGCACCCATGTGTATTACGGCGGTGCATTCCACAACGGCGCAGCCGTGGTGGTGCTGGTCAACACCTGGCTCGACTTCGAAGTGTTCACCACGCATCACATCCTGCCGCGCCAGGAAAAGCTGGTGGTAACCGGTGCCGACAGCGCCCAGCGCCGGGTTTTCGAACTGAATGCCGAGCCGGCGGCCGAG  >  NC_002947/3050594‑3050882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑