| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,092,131 | C→G | 6.6% | A31G (GCG→GGG) | PP_2701 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,092,131 | 0 | C | G | 6.6% | 195.4 / 3.9 | 61 | A31G (GCG→GGG) | PP_2701 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (30/27); new base G (2/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
GGGCTGCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCC > NC_002947/3091989‑3092271 | gggCTGCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAg > 2:23575/1‑149 (MQ=255) ggCTGCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGc > 6:14674/1‑149 (MQ=255) tGCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCGCGGGCTGAgtaat > 8:229985/1‑145 (MQ=255) gCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCt > 1:195763/1‑149 (MQ=255) ccAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCGCGGGCTGAgtaat < 7:229985/147‑5 (MQ=255) ccAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGc > 8:212726/1‑144 (MQ=255) ccAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGc < 7:212726/144‑1 (MQ=255) ccAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTc < 6:380160/149‑1 (MQ=255) ccAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTc > 8:3879/1‑149 (MQ=255) gAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAGCTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGAt > 6:126223/1‑149 (MQ=255) cacCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATc > 3:215463/1‑147 (MQ=255) ccTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAg > 7:186077/1‑148 (MQ=255) cTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAg > 5:447/1‑147 (MQ=255) cTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGa < 2:133925/149‑1 (MQ=255) tttCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAg > 2:187030/1‑149 (MQ=255) gAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTg > 1:195182/1‑148 (MQ=255) gAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTg > 5:148463/1‑148 (MQ=255) ggCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCgg < 6:174123/149‑1 (MQ=255) cGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAg < 6:447/149‑1 (MQ=255) gCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAg < 2:27836/136‑1 (MQ=255) gCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAg > 1:27836/1‑136 (MQ=255) gCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAgg > 1:5891/1‑149 (MQ=255) cTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGt < 6:148463/149‑1 (MQ=255) aacaacAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAg > 3:336430/1‑149 (MQ=255) ggTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTAcc < 8:186077/149‑1 (MQ=255) aGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGtt < 5:175539/149‑1 (MQ=255) aGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGtt < 5:14674/149‑1 (MQ=255) cGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCa < 2:195182/149‑1 (MQ=255) ggTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAg < 4:215463/149‑1 (MQ=255) ttGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCg > 7:209342/1‑149 (MQ=255) gAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGt > 3:354649/1‑149 (MQ=255) ttCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGca > 4:72634/1‑148 (MQ=255) tCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTAcc < 4:13688/130‑1 (MQ=255) tCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTAcc > 3:13688/1‑130 (MQ=255) aGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATg < 2:195763/147‑1 (MQ=255) gggCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGcc < 1:187030/149‑1 (MQ=255) ggCGCTGGTCTCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCATGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGccc < 5:126223/149‑1 (MQ=255) ggCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGccc < 4:354649/149‑1 (MQ=255) cTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTAc < 3:72634/149‑1 (MQ=255) gTCGGCATTGATAACGCAGGTAGCATCCCCGAAGCCTCCAAGCTCATGCACATTACTCAGGCCGCGTTGAGCCGGTCGATCAAGGAGCTGGAGCGGGAGGTATGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGa < 8:52311/149‑1 (MQ=255) cATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAg < 1:23575/149‑1 (MQ=255) cATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAg < 7:3879/149‑1 (MQ=255) aCGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCgt > 6:262582/1‑149 (MQ=255) cGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGGTTACCCGGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCgtg > 5:105291/1‑149 (MQ=255) aTCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCa > 1:252870/1‑149 (MQ=255) aTCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCa < 2:5891/149‑1 (MQ=255) tCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCAt < 5:129024/149‑1 (MQ=255) tCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGc > 5:250757/1‑148 (MQ=255) gAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGc > 7:144006/1‑91 (MQ=255) gAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGc < 8:144006/91‑1 (MQ=255) tCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGt > 1:13141/1‑149 (MQ=255) tCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGt > 3:366323/1‑149 (MQ=255) cAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGt < 4:336430/147‑1 (MQ=255) tCATGCACATTACTCAGCCCGCGCTGAGCCGATCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTa > 1:226958/1‑146 (MQ=255) tGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTagagag > 7:73475/1‑148 (MQ=255) caTTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATAc > 4:356982/1‑149 (MQ=255) aTTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATgg < 3:84080/88‑1 (MQ=255) aTTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATgg > 4:84080/1‑88 (MQ=255) gcTCAGCGCGGGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATAcgc > 7:246756/2‑147 (MQ=255) gcTCAGCGCGGGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATAcgc < 8:246756/146‑1 (MQ=255) aGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACgccgcc < 8:209342/147‑1 (MQ=255) | GGGCTGCCAGAACACCTTTCGAACCGGCCGCTAACAACAAGGTACAGACGGTTGAAACTTCACCAACTCAGGGCGCTGGTCGCCATTCATAACGCAGGTAGCATCCTCGAAGCCTCCAAGCTCATGCACATTACTCAGCCCGCGCTGAGCCGCTCGATCAAGGAGCTGGAGCGGGAGGTAGGGTTTACCCTGTTGCAGCGGTCGCACAAAGGCATGGCCCTTACCGAGGAAGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCC > NC_002947/3091989‑3092271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |