Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,155,364 C→G 11.6% G300A (GGC→GCC)  PP_2771 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,155,3640CG11.6% 121.7 / 7.4 43G300A (GGC→GCC) PP_2771sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (24/14);  new base G (3/2);  total (27/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

CCAGTTCGCCGATGGCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACA  >  NC_002947/3155224‑3155511
                                                                                                                                             |                                                                                                                                                   
ccaGTTCGCCGATGGCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCAGCCGGCCTGc                                                                                                                                             >  1:208816/1‑149 (MQ=255)
      cGCCGATGGCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGctcctc                                                                                                                                        <  8:34579/148‑1 (MQ=255)
         cGATGGCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGttcgaggag                                                                                                                                     >  8:304020/1‑142 (MQ=255)
                    aGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGaa                                                                                                                          <  7:54659/148‑1 (MQ=255)
                            gcgGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCg                                                                                                                   >  2:101688/1‑147 (MQ=255)
                               ggTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCgca                                                                                                                 >  5:179631/1‑146 (MQ=255)
                               ggTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCgca                                                                                                                 <  6:179631/146‑1 (MQ=255)
                                   aTGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTg                                                                                                          >  4:259507/1‑149 (MQ=255)
                                           gCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAGCGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCg                                                                                                   <  1:101688/149‑1 (MQ=255)
                                             tCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGgcg                                                                                                >  2:369707/1‑149 (MQ=255)
                                                    cagcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcgg                                                                                         >  7:178077/1‑149 (MQ=255)
                                                    cagcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcgg                                                                                         <  5:289690/149‑1 (MQ=255)
                                                     agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggc                                                                                        >  7:262707/1‑149 (MQ=255)
                                                       cagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggcgg                                                                                      >  3:160930/1‑149 (MQ=255)
                                                        agGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGt                                                                                     >  6:209747/1‑149 (MQ=255)
                                                              gCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCAc                                                                               >  6:320147/1‑149 (MQ=255)
                                                                      ctccTGGTTGCAGTGGCAGAAACGATAGTCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGAc                                                                        <  1:46595/146‑1 (MQ=255)
                                                                               gCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTgg                                                               <  8:262707/148‑1 (MQ=255)
                                                                                             gATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCgca                                                                                                                 >  6:20585/1‑84 (MQ=255)
                                                                                             gATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCgca                                                                                                                 <  5:20585/84‑1 (MQ=255)
                                                                                             gATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAgc                                                >  1:117233/1‑148 (MQ=255)
                                                                                                  gCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGa                                           <  2:208816/149‑1 (MQ=255)
                                                                                                          tAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCa                                   <  8:178077/149‑1 (MQ=255)
                                                                                                            gCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGc                                             >  4:209118/1‑137 (MQ=255)
                                                                                                            gCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGc                                             <  3:209118/137‑1 (MQ=255)
                                                                                                               ggCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgtcg                              >  3:216636/1‑149 (MQ=255)
                                                                                                                    tCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgtcgtcgtc                         >  4:189618/1‑149 (MQ=255)
                                                                                                                      cTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCt                       >  3:174793/1‑149 (MQ=255)
                                                                                                                      cTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCt                       >  8:235496/1‑149 (MQ=255)
                                                                                                                       tCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTg                      >  3:314638/1‑149 (MQ=255)
                                                                                                                            cTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCAc                   >  4:342781/1‑147 (MQ=255)
                                                                                                                                 ggagCAG‑GCCGGCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGc                     >  2:72376/4‑140 (MQ=255)
                                                                                                                                 ggagCAG‑GCCGGCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGc                     <  1:72376/137‑1 (MQ=255)
                                                                                                                                  gttcAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGc              <  3:264129/143‑1 (MQ=255)
                                                                                                                                  gagCAG‑GCCGGCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACtt                                                                      >  2:339884/3‑90 (MQ=255)
                                                                                                                                  gTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAt           >  5:195091/1‑149 (MQ=255)
                                                                                                                                  gTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAt           >  1:269641/1‑149 (MQ=255)
                                                                                                                                  gTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAt           >  6:26475/1‑149 (MQ=255)
                                                                                                                                   tGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAtt          <  4:160930/149‑1 (MQ=255)
                                                                                                                                    gCAG‑GCCGGCGGCCTGCACCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCa                                   >  3:214134/1‑123 (MQ=255)
                                                                                                                                    gCAG‑GCCGGCGGCCTGCACCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCa                                   <  4:214134/123‑1 (MQ=255)
                                                                                                                                    gCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCa                    <  2:270992/138‑1 (MQ=255)
                                                                                                                                    gCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCa                    >  1:270992/1‑138 (MQ=255)
                                                                                                                                           cgccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGca    >  6:103558/1‑148 (MQ=255)
                                                                                                                                           cgccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGca    >  4:243526/1‑148 (MQ=255)
                                                                                                                                           cgccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTCCGGCGGTGCTCAACGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGca    >  7:297836/1‑148 (MQ=255)
                                                                                                                                            gccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGcaca  >  3:254499/1‑149 (MQ=255)
                                                                                                                                            gccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGcaca  <  3:259507/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCAGTTCGCCGATGGCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCTCGACCTTCTCGTGCAG‑GCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACA  >  NC_002947/3155224‑3155511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑