Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,199,088 G→A 8.5% L143L (CTG→TTG)  PP_2806 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,199,0880GA 8.5% 134.0 / 2.8 47L143L (CTG→TTG) PP_2806TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base A (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCA  >  NC_002947/3198951‑3199235
                                                                                                                                         |                                                                                                                                                   
cAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCgg                                                                                                                                          <  2:103436/149‑1 (MQ=255)
           ccTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCAc                                                                                                                                >  3:233783/1‑148 (MQ=255)
                     ggtctcggtctcGGCCTCCAGGCGTGCACGGGCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGtt                                                                                                                     <  7:261715/149‑1 (MQ=255)
                         tcggtctcggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGc                                                                                                                 >  5:43456/1‑149 (MQ=255)
                               tcggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTc                                                                                                           >  7:92815/1‑149 (MQ=255)
                                 ggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGa                                                                                                         <  1:36239/149‑1 (MQ=255)
                                 ggCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGa                                                                                                         <  7:19618/149‑1 (MQ=255)
                                  gCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATGAGCAAGCGGGTGATAGTGGTCAGTTCTGCCTGGTGCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAg                                                                                                        <  2:85335/149‑1 (MQ=255)
                                   ccTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAgg                                                                                                       <  4:233783/149‑1 (MQ=255)
                                   ccTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAATGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAgg                                                                                                       >  7:80121/1‑149 (MQ=255)
                                   ccTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAATGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAgg                                                                                                       >  7:85100/1‑149 (MQ=255)
                                          gCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACgg                                                                                                >  6:372146/1‑149 (MQ=255)
                                             tGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCa                                                                                              >  7:101012/1‑148 (MQ=255)
                                              gCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATa                                                                                            <  8:113682/149‑1 (MQ=255)
                                               cACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATa                                                                                            <  5:372146/148‑1 (MQ=255)
                                                 cGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCa                                                                                              >  5:117414/1‑144 (MQ=255)
                                                     cGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTg                                                                                     <  5:372025/149‑1 (MQ=255)
                                                        cGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGtt                                                                                  <  7:219524/149‑1 (MQ=255)
                                                            aGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGcc                                                                               <  6:117414/148‑1 (MQ=255)
                                                                  ccAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCg                                                                        >  3:131961/1‑149 (MQ=255)
                                                                    aGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCggg                                                                      >  4:110180/1‑149 (MQ=255)
                                                                        gAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTg                                                                                     >  1:255412/1‑130 (MQ=255)
                                                                        gAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTg                                                                                     <  2:255412/130‑1 (MQ=255)
                                                                                ggCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGc                                                                                                                          <  3:143063/85‑1 (MQ=255)
                                                                                ggCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGc                                                                                                                          >  4:143063/1‑85 (MQ=255)
                                                                                       gAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGc                                                   <  6:43456/149‑1 (MQ=255)
                                                                                           aGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCaaaa                                               >  5:202215/1‑149 (MQ=255)
                                                                                            gATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCaaaa                                               >  6:46374/1‑148 (MQ=255)
                                                                                                 aTCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAATGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGtt                                          <  8:85100/148‑1 (MQ=255)
                                                                                                 aTCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAATGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGtt                                          <  8:80121/148‑1 (MQ=255)
                                                                                                  tCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTc                                         >  1:121962/1‑148 (MQ=255)
                                                                                                    aGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGaa                                      <  4:131961/149‑1 (MQ=255)
                                                                                                              tGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAg                                            >  3:232255/1‑133 (MQ=255)
                                                                                                              tGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAg                                            <  4:232255/133‑1 (MQ=255)
                                                                                                              tGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTc                            >  3:18146/1‑149 (MQ=255)
                                                                                                              tGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTc                            >  2:230906/1‑149 (MQ=255)
                                                                                                                aTAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTc                            <  6:202215/147‑1 (MQ=255)
                                                                                                                 tAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGcc                         <  8:101012/149‑1 (MQ=255)
                                                                                                                      gTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCAtct                     >  4:130823/1‑148 (MQ=255)
                                                                                                                      gTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCAtct                     >  3:325646/1‑148 (MQ=255)
                                                                                                                         aGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAgg                 <  3:110180/149‑1 (MQ=255)
                                                                                                                          gTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGa                >  7:79661/1‑149 (MQ=255)
                                                                                                                                  tGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGc                                                                                >  4:178031/1‑77 (MQ=255)
                                                                                                                                  tGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGc                                                                                <  3:178031/77‑1 (MQ=255)
                                                                                                                                      ccAGTGCTCCGTCGGTGACCTTAACGATGCCCTGTTGGGCCGCTTCGACGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTCCGTCGTCGCAATCACAGGATGACCGGTGAAc    >  4:326436/1‑149 (MQ=255)
                                                                                                                                      ccAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAAc    >  7:88192/1‑149 (MQ=255)
                                                                                                                                        aGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCa  <  2:121962/149‑1 (MQ=255)
                                                                                                                                         gTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGa             >  3:205587/1‑137 (MQ=255)
                                                                                                                                         gTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGa             <  4:205587/137‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAAGCGGCTGATAGTGGTCAGTGCTGCCTGGTCCAGTGCGCCGTCGGTGACCTTCACGATGGCCTGTTGGGCCGCTTCGAGGGAAACGGCATAGATCTTGGTTGCCATCTCCGGGTTGTTGCGCAGGTTCCAGCAAAACAGTTCGAACTGCGTGGTCGCCATCTCAGGATGAGCGGTGAACCA  >  NC_002947/3198951‑3199235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑