Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,207,241 T→C 6.6% V297A (GTG→GCG)  PP_2812 → transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,207,2410TC 6.6% 170.6 / 2.7 60V297A (GTG→GCG) PP_2812transporter
Reads supporting (aligned to +/- strand):  ref base T (28/28);  new base C (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

TTGCTTCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATACCGGGAACACTGGCCTTGGT  >  NC_002947/3207093‑3207388
                                                                                                                                                    |                                                                                                                                                   
ttGCTTCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGGTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGt                                                                                                                                                     >  5:101012/1‑149 (MQ=255)
  gCTTCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTg                                                                                                                                                    <  5:191505/148‑1 (MQ=255)
    ttCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGccc                                                                                                                                                 >  1:95308/1‑149 (MQ=255)
    ttCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGccc                                                                                                                                                 >  2:2981/1‑149 (MQ=255)
        gACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTcc                                                                                                                                             >  6:329538/1‑149 (MQ=255)
         aCCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCt                                                                                                                                            <  1:2981/149‑1 (MQ=255)
              gggggCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGgtgt                                                                                                                                       >  4:308364/1‑149 (MQ=255)
                 ggCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCg                                                                                                                                    <  8:193299/149‑1 (MQ=255)
                      tgtACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATc                                                                                                                               >  1:182834/1‑149 (MQ=255)
                      tgtACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATc                                                                                                                               >  7:166615/1‑149 (MQ=255)
                        tACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCgg                                                                                                                             <  6:148162/149‑1 (MQ=255)
                             gtgttGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTgg                                                                                                                                          >  8:161695/4‑131 (MQ=255)
                             gtgttGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTgg                                                                                                                                          <  7:161695/128‑1 (MQ=255)
                                  gCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCAt                                                                                                                   >  2:293055/1‑149 (MQ=255)
                                   ccACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATg                                                                                                                  <  2:182834/149‑1 (MQ=255)
                                   ccACTCACTGCGCTTCACGCTGCTGGCGCTGGTCCGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCAt                                                                                                                   <  3:216218/148‑1 (MQ=255)
                                     actcactGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGATGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGc                                                                                                                >  1:118958/1‑149 (MQ=255)
                                      ctcactGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGATCGACCCGTTGGCGGTGCTGGTGACCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCg                                                                                                               >  8:38410/1‑149 (MQ=255)
                                          ctGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTgca                                                                                                           <  5:329538/149‑1 (MQ=255)
                                           tGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCGGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTgcag                                                                                                          >  3:11/1‑149 (MQ=255)
                                               cTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCaaa                                                                                                      <  2:277385/149‑1 (MQ=255)
                                                ttCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAAt                                                                                                     >  3:122323/1‑149 (MQ=255)
                                                    cGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAAc                                                                                                 <  3:308364/149‑1 (MQ=255)
                                                    cGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAAc                                                                                                 <  2:95308/149‑1 (MQ=255)
                                                    cGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCCGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAAc                                                                                                 <  4:11/149‑1 (MQ=255)
                                                     gctgctGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGAGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACt                                                                                                >  1:354710/1‑149 (MQ=255)
                                                       tgctgGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGCGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACtt                                                                                               >  7:19065/1‑148 (MQ=255)
                                                       tgctgGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGCGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACtt                                                                                               >  7:13603/1‑148 (MQ=255)
                                                         ctgGCGCTGGTCTGTGCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTc                                                                                              <  5:257299/147‑1 (MQ=255)
                                                          tgGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGAGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATc                                                                                           <  2:354710/149‑1 (MQ=255)
                                                                tGGTCTGTTCGCTGTCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCGTGTTGGGGTACGGGGTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTGGCCATCTGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTgcgc                                                                                     <  7:62192/149‑1 (MQ=255)
                                                                tGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTg                                                                                                                                           >  1:72243/1‑95 (MQ=255)
                                                                tGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTg                                                                                                                                           <  2:72243/95‑1 (MQ=255)
                                                                  gTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCga                                                                                   <  8:166615/149‑1 (MQ=255)
                                                                  gTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCga                                                                                   <  2:118958/149‑1 (MQ=255)
                                                                       ttCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCg                                                                              >  4:198702/1‑149 (MQ=255)
                                                                          gCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTa                                                                           >  6:212592/1‑149 (MQ=255)
                                                                          gCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTGCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTa                                                                           >  2:252117/1‑149 (MQ=255)
                                                                               ccTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCgg                                                                       >  7:323107/1‑148 (MQ=255)
                                                                                      ggTGTGGCAGTTCAGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTg                                                               >  4:74888/1‑149 (MQ=255)
                                                                                            gCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCg                                                            >  7:186792/1‑146 (MQ=255)
                                                                                             cAGTTCGGCAGCCTGCGCCTGTGGGGGGACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGGTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAaga                                                        <  6:101012/149‑1 (MQ=255)
                                                                                              aGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAagag                                                       >  4:124748/1‑149 (MQ=255)
                                                                                               gTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAgg                                                      <  3:198702/149‑1 (MQ=255)
                                                                                                    gCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGgcggcg                                                 >  5:163976/1‑149 (MQ=255)
                                                                                                    gCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGgcggcg                                                 >  4:95726/1‑149 (MQ=255)
                                                                                                     cAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTg                                                                                                             >  8:146991/1‑88 (MQ=255)
                                                                                                     cAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTg                                                                                                             <  7:146991/88‑1 (MQ=255)
                                                                                                            cgcCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGa                                          <  3:74888/148‑1 (MQ=255)
                                                                                                               cTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCtt                                      >  6:239145/1‑149 (MQ=255)
                                                                                                                    ggggTACGGGCTCGACCCGTTGGCGGTGCTGGCGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGTGCTTCAcc                                  <  8:19065/148‑1 (MQ=255)
                                                                                                                    ggggTACGGGCTCGACCCGTTGGCGGTGCTGGCGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGTGCTTCAcc                                  <  8:13603/148‑1 (MQ=255)
                                                                                                                           gggCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCt                          <  6:318439/149‑1 (MQ=255)
                                                                                                                           gggCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCt                          >  5:318439/1‑149 (MQ=255)
                                                                                                                             gCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGa                        <  5:239145/149‑1 (MQ=255)
                                                                                                                               tCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATa                      >  4:112552/1‑149 (MQ=255)
                                                                                                                                         ggCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAAc                                                                                                 >  1:166133/1‑64 (MQ=255)
                                                                                                                                         ggCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAAc                                                                                                 <  2:166133/64‑1 (MQ=255)
                                                                                                                                          gCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATACCGGGAACACt           <  8:323107/149‑1 (MQ=255)
                                                                                                                                                   gTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATACCGGGAACACTGGCCTTGGt  <  2:101096/149‑1 (MQ=255)
                                                                                                                                                   gTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATACCGGGAACACTGGCCTTGGt  <  4:122323/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGCTTCTGACCGCGGGGGCGGTGTACTGGTATTGCCACTCACTGCGCTTCACGCTGCTGGCGCTGGTCTGTTCGCTGGCCTCGCTGGTGTGGCAGTTCGGCAGCCTGCGCCTGTTGGGGTACGGGCTCGACCCGTTGGCGGTGCTGGTGCCCTTCCTGGTGTTCGCCATCGGGGTTTCCCATGGCGTGCAGCAAATCAACTTCATCGTGCGCGAGATCGCTATCGGCAAGAGTGCCGAAGAGGCGGCGCGCTCGAGCTTCACCGGCTTGCTGATACCGGGAACACTGGCCTTGGT  >  NC_002947/3207093‑3207388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑