Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 253,348 T→C 6.2% intergenic (‑133/+38) PP_0202 ← / ← PP_0203 CBS domain‑containing protein/dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947253,3480TC 6.2% 236.6 / 4.6 80intergenic (‑133/+38)PP_0202/PP_0203CBS domain‑containing protein/dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base C (3/2);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

TTCGACGGTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTAC  >  NC_002947/253201‑253494
                                                                                                                                                   |                                                                                                                                                  
ttCGACGGTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCttt                                                                                                                                                   >  2:345200/1‑149 (MQ=255)
   gACGGTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTcg                                                                                                                                                >  6:320307/1‑149 (MQ=255)
    aCGGTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTAGCTTGTAGCGGCCTTTTcgc                                                                                                                                               >  7:319537/1‑149 (MQ=255)
       gTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGc                                                                                                                                            <  4:217054/149‑1 (MQ=255)
            cATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTaa                                                                                                                                         <  7:170666/147‑1 (MQ=255)
                 gggCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACcgc                                                                                                                                   >  7:347582/1‑148 (MQ=255)
                     ctctGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGaa                                                                                                                                >  1:79187/1‑147 (MQ=255)
                     ctctGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAaga                                                                                                                              <  2:240074/149‑1 (MQ=255)
                       ctGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGaa                                                                                                                                <  5:168382/145‑1 (MQ=255)
                       ctGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGaa                                                                                                                                >  6:168382/1‑145 (MQ=255)
                        tGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTACCGGCCTCTTCGCGGTTTTAGCCGCGAaaag                                                                                                                             <  3:123201/147‑1 (MQ=255)
                         gCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGcc                                                                                                                          >  1:48746/1‑149 (MQ=255)
                          ctggTGAAAAAAGCACTGTCTCGGAGTAACAAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGAGTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGTTTTAGCCGCGAAAAGGcc                                                                                                                          <  6:264711/146‑1 (MQ=255)
                          cGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCg                                                                                                                         <  3:248322/149‑1 (MQ=255)
                                aaaaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTg                                                                                                                   >  6:129360/1‑149 (MQ=255)
                                 aaaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTg                                                                                                                   <  8:347582/148‑1 (MQ=255)
                                       cACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATc                                                                                                             >  2:72249/1‑148 (MQ=255)
                                         cTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACTGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaaa                                                                                                          <  8:26458/149‑1 (MQ=255)
                                         cTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaaa                                                                                                          <  7:183757/149‑1 (MQ=255)
                                         cTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCa                                                                                                            >  4:22105/1‑147 (MQ=255)
                                            tctcGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAg                                                                                                         <  5:129360/147‑1 (MQ=255)
                                               cGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCg                                                                                                    >  4:315340/1‑149 (MQ=255)
                                                ggAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaa                                                                                                           >  5:221403/1‑141 (MQ=255)
                                                ggAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaa                                                                                                           <  6:221403/141‑1 (MQ=255)
                                                ggAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTc                                                                                                     <  1:345200/147‑1 (MQ=255)
                                                ggAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTc                                                                                                     <  1:363146/147‑1 (MQ=255)
                                                 gAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGc                                                                                                  <  1:72249/149‑1 (MQ=255)
                                                      aCTAACGGATCCGGTACGTCGATCTTCACGGAAATGCATGAAAGGGCATTGTGGCGTTTGAAATACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTg                                                                                             <  7:71484/149‑1 (MQ=255)
                                                      aCTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCt                                                                                              <  4:299736/148‑1 (MQ=255)
                                                       cTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGc                                                                                            <  2:48746/149‑1 (MQ=255)
                                                            ccATCCGTTACGGCGCTCTGCACGGCAATGAAGTAAACGGCAGGGAGGCTATTTAAAAACGTCATGGCTGTGCGCTTGTAGCGGCCTTTTCGCGGCTAAACCCGCTAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGAt                                                                                       <  2:182598/148‑1 (MQ=255)
                                                             cATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATc                                                                                      >  5:293514/1‑149 (MQ=255)
                                                              aTCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATcc                                                                                     >  1:149067/1‑149 (MQ=255)
                                                                 cGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCcgc                                                                                   <  6:293514/148‑1 (MQ=255)
                                                                    tACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGcca                                                                               >  8:122053/1‑149 (MQ=255)
                                                                    tACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGcca                                                                               <  2:79187/149‑1 (MQ=255)
                                                                          gcTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCAttt                                                                         <  2:149067/149‑1 (MQ=255)
                                                                              tGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCg                                                                       <  5:224622/147‑1 (MQ=255)
                                                                                cACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCt                                                                     <  5:320307/147‑1 (MQ=255)
                                                                                cACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTca                                                                   >  3:66328/1‑149 (MQ=255)
                                                                                      aaTGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCt                                                             <  3:315340/149‑1 (MQ=255)
                                                                                         gCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGc                                                           >  8:92307/1‑148 (MQ=255)
                                                                                           aGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAg                                                        <  3:316794/149‑1 (MQ=255)
                                                                                             gAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTAGGCATCTGAGATATCCGCGCCACCATTTCGATCACCCGCTGCAAGTc                                                      >  6:127350/1‑149 (MQ=255)
                                                                                               aaCGGCATGGATGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCa                                                    <  7:122053/149‑1 (MQ=255)
                                                                                               aaCGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTc                                                      >  5:199787/1‑147 (MQ=255)
                                                                                                  ggCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTcc                                                     >  1:273323/1‑145 (MQ=255)
                                                                                                  ggCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTcc                                                     <  2:273323/145‑1 (MQ=255)
                                                                                                         aGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTg                                          >  1:57623/1‑149 (MQ=255)
                                                                                                             gATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTc                                       >  3:124666/1‑148 (MQ=255)
                                                                                                                  aaaaaCGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCCCCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGt                                 >  2:357201/1‑149 (MQ=255)
                                                                                                                  aaaaaCGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCg                                                                       <  6:67241/111‑1 (MQ=255)
                                                                                                                  aaaaaCGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCg                                                                       >  5:67241/1‑111 (MQ=255)
                                                                                                                     accggcATGGCTGTTCGGTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCtt                              <  8:319537/144‑1 (MQ=255)
                                                                                                                        gTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGaa                           <  3:22105/149‑1 (MQ=255)
                                                                                                                         tCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                          >  3:363814/1‑149 (MQ=255)
                                                                                                                           aTGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtc                        <  4:255570/149‑1 (MQ=255)
                                                                                                                           aTGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtc                        >  3:255570/1‑149 (MQ=255)
                                                                                                                            tGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcg                       >  3:325302/1‑149 (MQ=255)
                                                                                                                             ggCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgt                      >  3:364277/1‑149 (MQ=255)
                                                                                                                             ggCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgt                      >  7:156213/1‑149 (MQ=255)
                                                                                                                              gCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgtt                     <  8:156213/149‑1 (MQ=255)
                                                                                                                                    cGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                          <  2:320391/138‑1 (MQ=255)
                                                                                                                                    cGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                          <  2:325707/138‑1 (MQ=255)
                                                                                                                                    cGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                          >  1:320391/1‑138 (MQ=255)
                                                                                                                                    cGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCAACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                          >  1:325707/1‑138 (MQ=255)
                                                                                                                                     gCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgttgtcgtt               <  4:66328/148‑1 (MQ=255)
                                                                                                                                      cTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCCCCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTcgttgtcgttgt             >  3:132419/1‑149 (MQ=255)
                                                                                                                                       ttGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTtgtg            >  7:171474/1‑149 (MQ=255)
                                                                                                                                         gTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTtgtg            <  4:325302/147‑1 (MQ=255)
                                                                                                                                           aGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCACGATCCGCGCAACCATTTCGCTAACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTATTGAACTCGTTTGCGTTGTGCCCg        >  6:339312/1‑149 (MQ=255)
                                                                                                                                           aGCGGCCTTTTCGCGGCTAAAACAGCGAAGAGGCCGGTAATGCGCATCAAAGCATCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCAAGTTCTTGAACTCGTGGTGGTTGTGcgcg        >  2:355710/1‑149 (MQ=255)
                                                                                                                                             cGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGgt      <  4:364277/149‑1 (MQ=255)
                                                                                                                                              ggCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGACATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTcgttgtcgttgt             >  7:333129/1‑141 (MQ=255)
                                                                                                                                              ggCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGACATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTcgttgtcgttgt             <  8:333129/141‑1 (MQ=255)
                                                                                                                                              ggCCTCTTCGCGGTTTTAGCCGCGAAAAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAAc                                            <  3:256218/110‑1 (MQ=38)
                                                                                                                                              ggCCTCTTCGCGGTTTTAGCCGCGAAAAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAAc                                            >  4:256218/1‑110 (MQ=38)
                                                                                                                                              ggCCTCTTCGCGGTTTTAGCCGCGAAAAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGgtg     >  5:185550/1‑149 (MQ=255)
                                                                                                                                                ccTCTTCGCGGTTTTAGCCGCGAAAAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTa   >  4:142380/1‑149 (MQ=255)
                                                                                                                                                 cTTTTCGTGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTAc  >  8:47783/1‑149 (MQ=255)
                                                                                                                                                 cTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTAc  >  3:303756/1‑149 (MQ=255)
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TTCGACGGTCTTCATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCGGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTAC  >  NC_002947/253201‑253494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑