Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,338,476 C→A 7.0% D175Y (GAC→TAC)  PP_2935 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,338,4760CA 7.0% 178.8 / 3.2 57D175Y (GAC→TAC) PP_2935membrane protein
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base A (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

CCACACGGGGCAACCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGCCCTTGGGCGA  >  NC_002947/3338328‑3338622
                                                                                                                                                     |                                                                                                                                                  
ccACACGGGGCAACCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTc                                                                                                                                                    <  7:43977/149‑1 (MQ=255)
 cacaCGGGGCAACCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGCGGTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTc                                                                                                                                                    >  1:276388/1‑149 (MQ=255)
       gggCAACCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGc                                                                                                                                              >  4:61695/1‑148 (MQ=255)
            aCCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAAc                                                                                                                                        <  6:329026/149‑1 (MQ=255)
              ccGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTACACGGCCAGAACGc                                                                                                                                      >  8:186134/1‑149 (MQ=255)
              ccGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTACACGGCCAGAACGc                                                                                                                                      >  8:180881/1‑149 (MQ=255)
               cGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGcc                                                                                                                                     <  5:61865/149‑1 (MQ=255)
               cGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGc                                                                                                                                      >  6:186533/1‑148 (MQ=255)
                      aCGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCATAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGgc                                                                                                                              <  8:324668/149‑1 (MQ=255)
                      aCGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGgc                                                                                                                              >  2:293148/1‑149 (MQ=255)
                      aCGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGgc                                                                                                                              <  7:128895/149‑1 (MQ=255)
                               aGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATg                                                                                                                     >  1:275676/1‑149 (MQ=255)
                                gAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATgg                                                                                                                    <  1:104973/149‑1 (MQ=255)
                                gAAGGGCAGGCGCTCGCCGC‑GTAACACGCCCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCACAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCCGAACGCCAACTGGCGCACCGATgg                                                                                                                    >  5:115464/1‑149 (MQ=255)
                                   gggCAGGCGCTCGCCGC‑GTACCACGACCACCTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGa                                                                                                                 >  1:30476/1‑149 (MQ=255)
                                    ggCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCGAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGAt                                                                                                                >  3:302034/1‑149 (MQ=255)
                                     gCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCTATGGCgcga                                                                                                               >  6:111702/1‑146 (MQ=255)
                                       aGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATcgc                                                                                                             >  2:240768/1‑149 (MQ=255)
                                        ggCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGTCAACTGGCGCACCGATGGCGATcgcg                                                                                                            <  1:8721/149‑1 (MQ=255)
                                         gcgcTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGTCAGAACGCCAACTGGCGCACCGATGGCGATCgcgc                                                                                                           <  1:240768/149‑1 (MQ=255)
                                         gcgcTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGGCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCgcgc                                                                                                           <  7:119243/149‑1 (MQ=255)
                                         gcgcTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTGGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGgc                                                                                                                              <  7:113675/130‑1 (MQ=255)
                                             tCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCa                                                                                                         >  4:245075/1‑147 (MQ=255)
                                                ccgc‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATagga                                                                                                    <  4:177580/149‑1 (MQ=255)
                                                  cgagTACCACGACCACGTAGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCAAGCCCGTCCACGGCCAGAACGCCAAATGGCGCACCGATGGCGATCGCGCCATaggag                                                                                                   >  2:104232/4‑149 (MQ=255)
                                                   c‑gTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGc                                                                                                 <  5:186533/149‑1 (MQ=255)
                                                      tACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGa                                                                                               <  2:30476/149‑1 (MQ=255)
                                                         cacgaccacgTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATAc                                                                                            >  6:294269/1‑149 (MQ=255)
                                                            gaccacgTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGt                                                                                         >  1:127560/1‑149 (MQ=255)
                                                              ccacgTCGGGGCGCTTGCGAAGCACCCGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTc                                                                                       >  7:312179/1‑149 (MQ=255)
                                                               cacgTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCCCGCTGAAGTCCAGCCCGGCCCCGGCCAGCACGCCAACTGGCGCCCCGATGGCGATCGCGCGATAGGAGGCGATACCGTTcg                                                                                      >  6:19721/1‑148 (MQ=255)
                                                                acgTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCa                                                                                     <  3:40441/149‑1 (MQ=255)
                                                                     ggggCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCGAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACgaga                                                                                <  4:302034/149‑1 (MQ=255)
                                                                          gcTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGAcc                                                                           <  2:276388/149‑1 (MQ=255)
                                                                          gcTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGAcc                                                                           >  1:193588/1‑149 (MQ=255)
                                                                            ttGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGAccc                                                                          >  4:356046/1‑148 (MQ=255)
                                                                              gCGAAGCACGAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGAccc                                                                          <  3:356046/146‑1 (MQ=255)
                                                                              gCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTg                                                                        <  1:293148/148‑1 (MQ=255)
                                                                                gAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCAGAGGAGGCGATACCGTGCCACGCGCTGCCCCGGGcc                                                                     >  4:278209/1‑148 (MQ=255)
                                                                                  aGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAgt                                                                   >  4:153461/1‑149 (MQ=255)
                                                                                  aGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTACACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAgt                                                                   <  7:186134/149‑1 (MQ=255)
                                                                                  aGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTACACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAgt                                                                   <  7:180881/149‑1 (MQ=255)
                                                                                      ccAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGtgtg                                                                >  6:344554/1‑148 (MQ=255)
                                                                                             ggCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGc                                                        >  7:176086/1‑149 (MQ=255)
                                                                                                              ccagcaAAGCCGGCCCCCTCACGCTGAAGTCCAGCCCGGCCACGGCCAGAACGCCAACTGGCGCACCGCTGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGccc                                       <  3:278209/149‑1 (MQ=255)
                                                                                                                agcaAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGccc                                       >  3:134897/1‑147 (MQ=255)
                                                                                                                  caAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGc                                   >  7:229534/1‑149 (MQ=255)
                                                                                                                      gCCGGCCCCAGCTCGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGTGATGACCGTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCTGCTGaa                               <  2:193588/149‑1 (MQ=255)
                                                                                                                        cGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAgg                             <  8:176086/149‑1 (MQ=255)
                                                                                                                          gCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTg                           <  2:127560/149‑1 (MQ=255)
                                                                                                                            cccAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCTCCAGCTCAAGGTGGc                         <  8:312179/149‑1 (MQ=255)
                                                                                                                            cccAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGc                         <  5:294269/149‑1 (MQ=255)
                                                                                                                                 cACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGc                    <  5:344554/149‑1 (MQ=255)
                                                                                                                                         aGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGc            <  4:134897/149‑1 (MQ=255)
                                                                                                                                             cAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGCCCtt        >  6:371635/1‑149 (MQ=255)
                                                                                                                                             cAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGCCCtt        >  6:219965/1‑149 (MQ=255)
                                                                                                                                                cccGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGTGTCCGACCTGGCTGATGCCCCACCTCAAGGTGGCCACGCCAATCAGCCCTTggg     >  8:154033/1‑149 (MQ=255)
                                                                                                                                                    tCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGCCCTTgg      <  7:41198/144‑1 (MQ=255)
                                                                                                                                                    tCCACGGCCAGAACGCCAACTGGCGCACCGATCGCGATCGCGCGAAAGCAGGCGAAACCGTACCACGAGATGACCCTGGCACTGAGCACGGGGCCGACCTGGCTGATGACCCAGCTCAAGGACGCCACGCAGATCAGCCCTTGGgaga  >  8:41198/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCACACGGGGCAACCCGGCCGAACGCCGACCAGAAGGGCAGGCGCTCGCCGC‑GTACCACGACCACGTCGGGGCGCTTGCGAAGCACCAGCAGGGCAAGGGTTGCCAGCACCAGCAAAGCCGGCCCCAGCACGCTGAAGTCCAGCCCGTCCACGGCCAGAACGCCAACTGGCGCACCGATGGCGATCGCGCCATAGGAGGCGATACCGTTCCACGAGATGACCCTGGCAGTGTGCACGGGGCCGACCTGGCTGATGCCCCAGCTCAAGGTGGCCACGCCGATCAGCCCTTGGGCGA  >  NC_002947/3338328‑3338622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑