Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,385,250 T→C 8.1% E56G (GAG→GGG)  PP_2986 ← oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,385,2500TC 8.1% 138.0 / 2.6 49E56G (GAG→GGG) PP_2986oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (26/19);  new base C (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGACCCAACTTG  >  NC_002947/3385112‑3385398
                                                                                                                                          |                                                                                                                                                    
tttATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCgg                                                                                                                                            >  8:189204/1‑149 (MQ=255)
       gTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGACAGACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCGTGTCCCCAACGCCCAACTCCGCCCCCGGCAGgttg                                                                                                                                     >  4:351306/1‑149 (MQ=255)
          cGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGgttgtt                                                                                                                                   <  4:259488/148‑1 (MQ=255)
          cGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGgttgtt                                                                                                                                   <  7:284181/148‑1 (MQ=255)
           gAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGttgttg                                                                                                                                  >  7:18496/1‑148 (MQ=255)
              ttCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACCCCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGCCCAACTCCACACCCGGCAGGTTGTTGAAct                                                                                                                              >  6:303280/1‑149 (MQ=255)
              ttCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAAct                                                                                                                              >  5:286934/1‑149 (MQ=255)
               tCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAAct                                                                                                                              <  3:363011/148‑1 (MQ=255)
                  ttCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAActgctg                                                                                                                          >  5:45326/1‑149 (MQ=255)
                                 cccTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCttt                                                                                                           >  5:25783/1‑149 (MQ=255)
                                 cccTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCttt                                                                                                           <  4:92376/149‑1 (MQ=255)
                                  ccTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCtttt                                                                                                          >  2:328940/1‑149 (MQ=255)
                                      gTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCCCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGc                                                                                                      >  3:46966/1‑149 (MQ=255)
                                       tAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGCCCTCGGCCATGTCCCCATCGACGAGCTCCACACCCGGCAGGTGGATGAACTGCTGTTCGCCTTTGGCGTTTGcgcg                                                                                                     >  8:176578/1‑149 (MQ=255)
                                          ttATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGc                                                                                                      >  2:278351/1‑145 (MQ=255)
                                          ttATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGc                                                                                                      <  1:278351/145‑1 (MQ=255)
                                                 gAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACCCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCt                                                                                           >  2:245628/1‑149 (MQ=255)
                                                 gAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACCCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCt                                                                                           >  2:240470/1‑149 (MQ=255)
                                                    aGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTg                                                                                        <  6:286934/149‑1 (MQ=255)
                                                     gAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGa                                                                                       <  7:189204/149‑1 (MQ=255)
                                                         gacgCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCgt                                                                                   >  2:195994/1‑149 (MQ=255)
                                                          acgCTGTGTGAAACACCGTATCCCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCgtg                                                                                  <  4:46966/149‑1 (MQ=255)
                                                           cgCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCgtgt                                                                                 <  1:328940/149‑1 (MQ=255)
                                                            gCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTa                                                                                <  8:18496/149‑1 (MQ=255)
                                                             cTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGAcc                                                                                     <  5:377939/143‑1 (MQ=255)
                                                             cTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGAcc                                                                                     >  6:377939/1‑143 (MQ=255)
                                                                   gAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCcgc                                                                           >  8:275077/1‑147 (MQ=255)
                                                                    aaaCACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCgcga                                                                         <  1:195994/148‑1 (MQ=255)
                                                                        acCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGAc                                                                    >  5:343089/1‑149 (MQ=255)
                                                                         ccGTATAGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGAc                                                                    >  2:8341/1‑148 (MQ=255)
                                                                                tcAACCTTGCAGCGATTCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCTGGCCTTTGACCgtg                                                                                  <  3:115804/126‑1 (MQ=255)
                                                                                gCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCAc                                                            <  6:45326/149‑1 (MQ=255)
                                                                                gCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGCACCAGGCCTTTGACAgtg                                                                                  >  4:115804/1‑127 (MQ=255)
                                                                                  aaCCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACg                                                           >  5:349310/1‑148 (MQ=255)
                                                                                        gCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACCCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAg                                                     <  1:245628/148‑1 (MQ=255)
                                                                                        gCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACCCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAg                                                     <  1:240470/148‑1 (MQ=255)
                                                                                          aGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCtt                                                                                          >  7:318360/1‑109 (MQ=255)
                                                                                          aGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCtt                                                                                          <  8:318360/109‑1 (MQ=255)
                                                                                                           gTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCgg                                 >  2:261536/1‑149 (MQ=255)
                                                                                                            tCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGt                                <  6:349310/149‑1 (MQ=255)
                                                                                                                    ggCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCg                         <  3:351306/148‑1 (MQ=255)
                                                                                                                     gCCATGTCCCCACCGCCCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTAACCTTTGGCTTTAGAGCGTACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAACCCTCTTGCTCCGGt                       >  6:329226/1‑149 (MQ=255)
                                                                                                                     gCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGt                       <  6:25783/149‑1 (MQ=255)
                                                                                                                              ccAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACgccgcc              >  1:324610/1‑149 (MQ=255)
                                                                                                                               cAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCt             <  6:343089/149‑1 (MQ=255)
                                                                                                                                 aCGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGa           >  5:55102/1‑149 (MQ=255)
                                                                                                                                     ccAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGAccc        >  7:162459/1‑148 (MQ=255)
                                                                                                                                     ccAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGAccc        >  1:200526/1‑148 (MQ=255)
                                                                                                                                      cAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCCCGCCGCCTGACCCaa      >  2:311830/1‑149 (MQ=255)
                                                                                                                                          tCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGACCCAACTTg  >  2:16733/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
TTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATGTCCCCAACGACCAACTCCACACCCGGCAGGTTGTTGAACTGCTGTTCACCTTTGGCTTTTGAGCGGACCAGGCCTTTGACCGTGTAGCCGCGAGCGACCAATTCACGCACCAGGTTGTTGCCCAGCAAACCGGTTGCTCCGGTCACGCCGCCTGACCCAACTTG  >  NC_002947/3385112‑3385398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑