Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,388,807	C→T	8.3%	R291H (CGC→CAC) ‡	PP_2991 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,388,807	0	C	T	8.3%	207.1 / 5.7	72	R291H (CGC→CAC) ‡	PP_2991	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/34);  new base T (2/4);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

AATCAGGCCGGCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGAC  >  NC_002947/3388659‑3388952                                                                                                                                                     |                                                                                                                                                  aaTCAGGCCGGCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTcgc                                                                                                                                                   >  1:279579/1‑149 (MQ=255)      ggCCGGCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTc                                                                                                                                              >  4:114731/1‑149 (MQ=255)        ccGGCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGa                                                                                                                                            <  8:270595/149‑1 (MQ=255)           gCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGc                                                                                                                                          >  6:319729/1‑148 (MQ=255)             gAGTGGTTTGGGTGCTTAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAAATCGACAGAGCGCCGTCGCCTAGTGTGAGGNTGGCTGAATCTGCGTCGCGGCTCGAGCCAt                                                                                                                                       <  1:34970/149‑1 (MQ=255)                tGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGAc                                                                                                                                    <  2:367472/149‑1 (MQ=255)                  gTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCGCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACg                                                                                                                                   >  5:280545/1‑148 (MQ=255)                   tttGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTAGTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACg                                                                                                                                   >  1:259193/1‑147 (MQ=255)                   tttGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACg                                                                                                                                   >  3:154451/1‑147 (MQ=255)                   tctGGGGGCAGAGAGCGCACTCTGAACCGCTGGCACGTCTTCGTACTTCCTCATTTTCCCCAACTTCAGGGGGCGGCGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCGGAATCTGCGGCATGGCTCGAGCCGCGACGCa                                                                                                                                 <  4:298529/147‑1 (MQ=255)                      gggTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCCACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCAga                                                                                                                               <  1:14717/148‑2 (MQ=255)                         tGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGc                                                                                                                           <  4:167106/149‑1 (MQ=255)                         tGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGc                                                                                                                           <  7:40556/149‑1 (MQ=255)                            aTAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCa                                                                                                                                 <  7:16523/140‑1 (MQ=255)                            aTAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCa                                                                                                                                 >  8:16523/1‑140 (MQ=255)                             tAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCggg                                                                                                                        >  3:109147/1‑148 (MQ=255)                                agCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAAc                                                                                                                    >  7:144585/1‑149 (MQ=255)                                agCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAAc                                                                                                                    >  7:31389/1‑149 (MQ=255)                                  cgcACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCg                                                                                                                  <  4:109147/149‑1 (MQ=255)                                   gcACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGc                                                                                                                 <  8:31389/149‑1 (MQ=255)                                     aCTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCAt                                                                                                               <  8:144585/149‑1 (MQ=255)                                       tctGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATaa                                                                                                             >  3:332552/1‑149 (MQ=255)                                       tctGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCa                                                                                                                                 <  8:228380/129‑1 (MQ=255)                                       tctGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCa                                                                                                                                 >  7:228380/1‑129 (MQ=255)                                          gAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCg                                                                                                          >  4:176503/1‑149 (MQ=255)                                            aaCGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTa                                                                                                        <  8:238637/149‑1 (MQ=255)                                            aaCGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCACGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTa                                                                                                        <  6:280545/149‑1 (MQ=255)                                                                 ttCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCTTATGCCAACTGGCCCTGAGGATc                                                                                   >  8:248216/1‑149 (MQ=255)                                                                   catcatTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATc                                                                                   >  4:230736/1‑147 (MQ=255)                                                                    atcatTTTCCCGAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGATCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCga                                                                                 <  3:302139/148‑1 (MQ=255)                                                                    atcatTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGAGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGccc                                                                                           >  3:19512/1‑138 (MQ=255)                                                                    atcatTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGAGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGccc                                                                                           <  4:19512/138‑1 (MQ=255)                                                                        ttttCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGAt                                                                              <  7:340195/147‑1 (MQ=255)                                                                        ttttACCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGTGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAAATGGCCCTGATTATCgagaa                                                                              >  8:340195/1‑146 (MQ=255)                                                                        attaccccCACTTCAGGGTCGGGAGGTAAGTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCGATGACGCAGTTGGCGGGAAACCGCATAACCGTATCCCAACTGGCCCTGATGATCGAGATTg                                                                            <  2:315261/145‑1 (MQ=255)                                                                              ccAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAg                                                                      >  2:298593/1‑149 (MQ=255)                                                                                  cTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAgctgc                                                                  >  3:30638/1‑149 (MQ=255)                                                                                  cTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAgctgc                                                                  <  2:279579/149‑1 (MQ=255)                                                                                     cAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCt                                                                                          <  4:153351/122‑1 (MQ=255)                                                                                     cAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCt                                                                                          >  3:153351/1‑122 (MQ=255)                                                                                       gggTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGt                                                              >  5:333625/1‑148 (MQ=255)                                                                                        ggTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTTGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGa                                                            <  8:84256/149‑1 (MQ=255)                                                                                        ggTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCtg                                                                                         >  1:288293/1‑120 (MQ=255)                                                                                        ggTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCtg                                                                                         <  2:288293/120‑1 (MQ=255)                                                                                         gTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGAc                                                           >  3:318737/1‑149 (MQ=255)                                                                                            cGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGa                                                         >  5:345301/1‑148 (MQ=255)                                                                                             ggAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGata                                                       >  6:225316/1‑149 (MQ=255)                                                                                               aGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATc                                                     <  6:345301/149‑1 (MQ=255)                                                                                                 gTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGa                                                   <  2:259193/149‑1 (MQ=255)                                                                                                     ttCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTc                                               >  5:12223/1‑149 (MQ=255)                                                                                                     ttCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTc                                               <  3:176503/149‑1 (MQ=255)                                                                                                        gACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTcgc                                             >  1:269576/1‑148 (MQ=255)                                                                                                        gACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTcgc                                             <  3:230736/148‑1 (MQ=255)                                                                                                           agagCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCgaga                                         <  6:136985/149‑1 (MQ=255)                                                                                                           agagCGCCGGCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCgaga                                         <  4:71472/149‑1 (MQ=255)                                                                                                            gagCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCga                                           >  3:199936/1‑146 (MQ=255)                                                                                                                  cGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTg                                  <  6:12223/149‑1 (MQ=255)                                                                                                                        cTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGc                            >  7:259591/1‑149 (MQ=255)                                                                                                                         tAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGTGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGct                           >  4:180111/1‑149 (MQ=255)                                                                                                                           gtgtGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGct                           <  4:318737/147‑1 (MQ=255)                                                                                                                               gAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCg                     >  3:90610/1‑149 (MQ=255)                                                                                                                                 gggTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCt                   <  3:163180/149‑1 (MQ=255)                                                                                                                                  ggTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCtt                  <  5:225316/149‑1 (MQ=255)                                                                                                                                  ggTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGGCGCGCGTCTTCGCtt                  >  8:305287/1‑149 (MQ=255)                                                                                                                                    tGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCttt                 >  4:6262/1‑148 (MQ=255)                                                                                                                                      gCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGaa              <  3:114731/149‑1 (MQ=255)                                                                                                                                      gCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGaa              <  5:47091/149‑1 (MQ=255)                                                                                                                                       cTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAg             <  8:259591/149‑1 (MQ=255)                                                                                                                                          aaTCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGa           <  4:332552/148‑1 (MQ=255)                                                                                                                                               gCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTc     >  4:40317/1‑149 (MQ=255)                                                                                                                                                cGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGCTTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTc     >  7:265320/1‑148 (MQ=255)                                                                                                                                                cGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGacac        <  3:6262/145‑1 (MQ=255)                                                                                                                                                  tCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGAc  <  7:305287/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AATCAGGCCGGCGAGTGGTTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGAC  >  NC_002947/3388659‑3388952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑