Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,417,745	T→A	10.6%	E99V (GAA→GTA) ‡	PP_3030 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,417,745	0	T	A	10.6%	164.5 / 7.5	64	E99V (GAA→GTA) ‡	PP_3030	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (5/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATGGGGA  >  NC_002947/3417597‑3417889                                                                                                                                                     |                                                                                                                                                   aGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCtt                                                                                                                                                    <  1:344965/149‑1 (MQ=255)       gTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCgcag                                                                                                                                               >  5:123016/1‑148 (MQ=255)        tGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCgcagca                                                                                                                                             <  3:246461/149‑1 (MQ=255)        tGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGATGATGGGGGGAATAATGCCTGCTGCTTCgcag                                                                                                                                               >  6:127072/1‑147 (MQ=255)              taCCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACt                                                                                                                                       <  8:17623/149‑1 (MQ=255)                ccccACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACttt                                                                                                                                     >  4:182296/1‑149 (MQ=255)                 cccACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACtttt                                                                                                                                    >  8:11688/1‑149 (MQ=255)                   cACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTg                                                                                                                                  >  2:106568/1‑149 (MQ=255)                   cACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTg                                                                                                                                  >  7:319977/1‑149 (MQ=255)                         aGGCTGCGCGTGCGGGCGACTGTTAGCGATTAGCGTTGCAGCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGtttttt                                                                                                                            <  3:273363/149‑1 (MQ=255)                           gCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGATACTTTTTGTTTTTTgc                                                                                                                          >  7:167279/1‑149 (MQ=255)                              gcgcGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGATTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGCGGGAATAATGCATGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTgcgat                                                                                                                       >  6:209558/1‑147 (MQ=255)                                     cGGGCGACTTTTAGCGATTAGCGTAGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGCCTGCTGATGGGGGCAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGacaa                                                                                                                <  5:127072/149‑1 (MQ=255)                                      gggCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGacaac                                                                                                               <  8:319977/149‑1 (MQ=255)                                         cGACTTTTTGGGATTAGGGTTGCATCAGCGGGGGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACg                                                                                                            <  8:167279/149‑1 (MQ=255)                                           aCTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGa                                                                                                          <  7:11688/149‑1 (MQ=255)                                             ttttAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACt                                                                                                        >  4:57687/1‑149 (MQ=255)                                                tAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTcg                                                                                                     >  4:331494/1‑149 (MQ=255)                                                tAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTc                                                                                                      <  3:172785/148‑1 (MQ=255)                                                   cGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTcgcg                                                                                                   >  1:369485/1‑148 (MQ=255)                                                     aTTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAg                                                                                                >  8:306279/1‑149 (MQ=255)                                                        aGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCg                                                                                              <  2:369485/148‑1 (MQ=255)                                                         gCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCg                                                                                              >  7:120413/1‑147 (MQ=255)                                                           gTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGc                                                                                          >  2:62270/1‑149 (MQ=255)                                                              gCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCTCCGCGAAgtcc                                                                                        <  4:183236/148‑4 (MQ=255)                                                               cATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCgg                                                                                        >  8:146212/1‑147 (MQ=255)                                                                aTCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAg                                                                                     >  7:279163/1‑149 (MQ=255)                                                                 tCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGt                                                                                    >  8:91895/1‑149 (MQ=255)                                                                   aGCGGGCGGTGCCTCTCGCAGCTTGTGGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGTGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTgg                                                                                  <  4:38960/149‑1 (MQ=255)                                                                    gcgggcggTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGc                                                                                 <  2:241674/149‑1 (MQ=255)                                                                           gTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACtt                                                                        >  1:67585/1‑149 (MQ=255)                                                                           gTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACtt                                                                        <  7:306279/149‑1 (MQ=255)                                                                                       gCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCt                                                            <  8:279163/149‑1 (MQ=255)                                                                                           gttgtGGGGTATCAGTGCCTCGGAGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCCGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTcg                                                        >  6:365763/1‑149 (MQ=255)                                                                                                        aGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTc                                            <  3:57687/148‑1 (MQ=255)                                                                                                        aGTGCCTAGGCGGGGCTGCTGATGGGGGGAATAATGCATGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCttac                                           >  3:205447/1‑147 (MQ=255)                                                                                                        aGTCCCTGGGCGGGGCTGCTGATGGGGGGATTAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTc                                            <  5:209558/148‑1 (MQ=255)                                                                                                          tGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCt                                         >  5:207519/1‑149 (MQ=255)                                                                                                                ggcggGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCggg                                    <  3:182296/148‑1 (MQ=255)                                                                                                                 gcggGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGt                                  >  8:116416/1‑149 (MQ=255)                                                                                                                  cggGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTc                                 <  6:123016/149‑1 (MQ=255)                                                                                                                    gggCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAg                               <  3:331494/149‑1 (MQ=255)                                                                                                                       ctgctgATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGAc                            <  2:67585/149‑1 (MQ=255)                                                                                                                        tgctgATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACt                           >  1:199847/1‑149 (MQ=255)                                                                                                                          cggATGGCGGTAATACTGCCTGCTGCTTCGCATCAGAGACCTTTTGTTTATTGCGCTTCCACAACACGGACTTCTCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTTCCCGCTTCCCTCCGGGGTCAGGACtt                          <  8:313253/146‑1 (MQ=255)                                                                                                                           tgATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTtgt                        >  3:93969/1‑149 (MQ=255)                                                                                                                                gggggAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTgtggtg                    <  8:120413/148‑1 (MQ=255)                                                                                                                                gggggAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTgtggtg                    <  5:336483/148‑1 (MQ=255)                                                                                                                                gggggAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGtggtgg                   >  2:103344/1‑149 (MQ=255)                                                                                                                                 ggggAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGAGAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCTACTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTgtggtg                    <  1:106568/149‑1 (MQ=255)                                                                                                                                      ataatGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTcc             <  7:146212/149‑1 (MQ=255)                                                                                                                                      ataatGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGCTATTcc             >  4:212829/1‑149 (MQ=255)                                                                                                                                       taatGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGA‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTcc             >  4:264184/1‑148 (MQ=255)                                                                                                                                         gtctctgctgCGAAGCAGCAGGGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCat          >  5:14842/5‑149 (MQ=255)                                                                                                                                         gtctctgctgCGAAGCAGCAGGGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCag          >  5:20040/5‑148 (MQ=255)                                                                                                                                          tctctgctgCGAAGCAGCAGAGACTTTTTTTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCat          >  3:266074/4‑148 (MQ=255)                                                                                                                                          tctctgctgCGAAGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGATTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCggg                                    >  5:60187/4‑122 (MQ=255)                                                                                                                                          tctctgctgCGAAGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGATTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCggg                                    <  6:60187/119‑1 (MQ=255)                                                                                                                                            tctgctgCGAAGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTgtggtg                    <  5:15912/135‑1 (MQ=255)                                                                                                                                            tctgctgCGAAGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTgtggtg                    >  6:15912/2‑136 (MQ=255)                                                                                                                                            ccTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGCCTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCatgat       <  1:62270/149‑1 (MQ=255)                                                                                                                                            ccTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCatgat       <  7:91895/149‑1 (MQ=255)                                                                                                                                              tgctgcTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATgg     >  2:50120/1‑149 (MQ=255)                                                                                                                                                ctgctTCGCAGCAGAGACTTTTTGTTTTTTGCGCTACGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTAGCCTGACCGCTTCCCTACGGGGTCAGGACTAGTGGTGGTATTCCCATGATggg    >  3:111159/1‑148 (MQ=255)                                                                                                                                                   ctTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATGGGGa  <  2:224700/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCAGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGC‑‑CTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATGGGGA  >  NC_002947/3417597‑3417889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑