Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,453,480	A→G	8.5%	intergenic (‑34/+79)	PP_3069 ← / ← ppiC‑I	outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,453,480	0	A	G	8.5%	166.4 / 5.0	59	intergenic (‑34/+79)	PP_3069/ppiC‑I	outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base G (3/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ACCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTA  >  NC_002947/3453333‑3453620                                                                                                                                                     |                                                                                                                                              aCCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGca                                                                                                                                               >  5:305404/1‑148 (MQ=255)   ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCGGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAg                                                                                                                                            >  6:151040/1‑149 (MQ=255)   ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTG‑GTGCGCAg                                                                                                                                            <  7:14926/149‑1 (MQ=255)    cgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAgg                                                                                                                                           >  6:38614/1‑149 (MQ=255)    cgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGGCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAgg                                                                                                                                           <  3:310418/149‑1 (MQ=255)             tGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCggg                                                                                                                                  <  1:289672/149‑1 (MQ=255)             tGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCggg                                                                                                                                  <  1:113014/149‑1 (MQ=255)                gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGtt                                                                                                                               <  5:338570/149‑1 (MQ=255)                gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGtt                                                                                                                               <  8:284087/149‑1 (MQ=255)                    gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGtat                                                                                                                           <  3:88921/149‑1 (MQ=255)                    gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGta                                                                                                                            >  5:17063/1‑148 (MQ=255)                      aCAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGta                                                                                                                            >  2:276994/1‑146 (MQ=255)                       cAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATaga                                                                                                                        <  6:112088/149‑1 (MQ=255)                        aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                       <  5:343740/149‑1 (MQ=255)                                  tgCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCa                                                                                                            <  5:38614/149‑1 (MQ=255)                                    cAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACg                                                                                                          <  8:337938/149‑1 (MQ=255)                                      gCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGAc                                                                                                        >  6:237723/1‑149 (MQ=255)                                            gAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGATGAGTGC‑g                                                                                                               <  8:65245/136‑1 (MQ=255)                                            gAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑g                                                                                                               >  7:65245/1‑136 (MQ=255)                                             aGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGTGAAACCTGCGCACGACGCGTT‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACt                                                                                                 <  6:240135/149‑1 (MQ=255)                                                ccATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGCTTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTgg                                                                                               <  1:276994/149‑1 (MQ=255)                                                 cATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGc                                                                                              >  5:183683/1‑148 (MQ=255)                                                   tGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCaaa                                                                                           <  4:347204/149‑1 (MQ=255)                                                            gcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAgg                                                                                  >  8:217475/1‑149 (MQ=255)                                                              gggAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGAAAAAATGTCAGGTg                                                                                >  5:280498/1‑149 (MQ=255)                                                                        ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGGGGCAAAGGGc                                                                       >  3:206328/1‑148 (MQ=255)                                                                         cAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTa                                                                     <  7:217475/149‑1 (MQ=255)                                                                         cAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGC‑AAAATGTCAGGTGGCAAAGGGCTAg                                                                    >  3:85373/1‑149 (MQ=255)                                                                                  cATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTC‑GAGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCAAGACCACAACTGGCAAAAATGTCAGCTGGCAAAGGGCAAGAGCCTTAg                                                            >  8:29139/1‑149 (MQ=255)                                                                                   aTCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGt                                                           >  7:93528/1‑149 (MQ=255)                                                                                        gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                      >  6:19534/1‑149 (MQ=255)                                                                                        gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                      <  7:29139/149‑1 (MQ=255)                                                                                        gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                      <  7:331333/149‑1 (MQ=255)                                                                                          gTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCa                                                                                    <  2:346118/117‑1 (MQ=255)                                                                                          gTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCa                                                                                    >  1:346118/1‑117 (MQ=255)                                                                                              aCGCATCATGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAAc                                                <  6:305404/149‑1 (MQ=255)                                                                                              aCGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAAc                                                <  6:17063/149‑1 (MQ=255)                                                                                                 cATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGtt                                             <  5:237723/149‑1 (MQ=255)                                                                                                  aTCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGttt                                            <  6:183683/149‑1 (MQ=255)                                                                                                     aGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCa                                         >  5:354409/1‑149 (MQ=255)                                                                                                     aGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCa                                         >  5:349187/1‑149 (MQ=255)                                                                                                      ggAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCAc                                        >  3:322008/1‑149 (MQ=255)                                                                                                       gAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGGTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCAc                                        >  1:275962/1‑148 (MQ=255)                                                                                                             cTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAgc                                                                   <  5:243402/115‑1 (MQ=255)                                                                                                             cTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAgc                                                                   >  6:243402/1‑115 (MQ=255)                                                                                                             cTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtgg                                 >  4:94605/1‑149 (MQ=255)                                                                                                              tCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTg                                           <  6:211809/138‑1 (MQ=255)                                                                                                              tCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTg                                           >  5:211809/1‑138 (MQ=255)                                                                                                                cATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGCGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAg                              <  4:206328/149‑1 (MQ=255)                                                                                                                 aTTCACCAGGGAAACCTGCGCACAACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTTGCAAAAATGTCAGGTGGCTAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGc                             >  4:238193/1‑149 (MQ=255)                                                                                                                         gggAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTg                     >  5:25123/1‑149 (MQ=255)                                                                                                                          ggAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGC‑AAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGct                   <  4:85373/149‑1 (MQ=255)                                                                                                                              aCCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCtt                <  5:19534/149‑1 (MQ=255)                                                                                                                                 tgtgcACCACGCGTG‑GTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                               >  5:299668/4‑131 (MQ=255)                                                                                                                                 tgtgcACCACGCGTG‑GTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                               <  6:299668/128‑1 (MQ=255)                                                                                                                                  gtgcACCACGCGTG‑GTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATc            >  4:270088/3‑149 (MQ=255)                                                                                                                                          cgcgTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCTAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGACCGTTTGCAGCAGGTGGTAGCCGAATTTGCTCTTGATAGGCCCgtg    >  8:87243/1‑149 (MQ=255)                                                                                                                                           gcgTG‑GTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCgtg    >  1:323225/1‑148 (MQ=255)                                                                                                                                            cgTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTa  <  5:151040/149‑1 (MQ=255)                                                                                                                                            cgTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTa  <  2:275962/149‑1 (MQ=255)                                                                                                                                                    cacaGGGTTACCGGGGTTGTATAGAGGAGTGCTGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGcc        >  1:44645/1‑137 (MQ=255)                                                                                                                                                    cacaGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGcc        <  2:44645/136‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              ACCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTG‑GTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGC‑GTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTA  >  NC_002947/3453333‑3453620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑